{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T11:09:19Z","timestamp":1790334559544,"version":"4.1.0"},"reference-count":12,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T00:00:00Z","timestamp":1783123200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>Prosaposin functions as a regulator of lysosomal enzymatic activity and as a neuroprotective player when secreted. Here we have characterized nine Prosaposin commercial antibodies for western blot and immunoprecipitation using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.<\/ns3:p>","DOI":"10.12688\/f1000research.177779.1","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T05:29:06Z","timestamp":1787290146000},"page":"1065","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A guide to selecting high-performing antibodies for Prosaposin (UniProt ID: P07602) for use in western blot and immunoprecipitation"],"prefix":"10.12688","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3728-3158","authenticated-orcid":false,"given":"Vera Ru\u00edz","family":"Mole\u00f3n","sequence":"first","affiliation":[{"name":"Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, Montreal Qu\u00e9bec H3A 2B4 McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4299-8281","authenticated-orcid":false,"given":"Sara Gonz\u00e1lez Bol\u00edvar","family":"Bol\u00edvar","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, Montreal Qu\u00e9bec H3A 2B4 McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riham","family":"Ayoubi","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, Montreal Qu\u00e9bec H3A 2B4 McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7535-8718","authenticated-orcid":false,"given":"Vincent","family":"Francis","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, Montreal Qu\u00e9bec H3A 2B4 McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter S.","family":"McPherson","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, Montreal Qu\u00e9bec H3A 2B4 McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5906-025X","authenticated-orcid":false,"given":"Carl","family":"Laflamme","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, Montreal Qu\u00e9bec H3A 2B4 McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2026,7,4]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1255-1267","DOI":"10.1016\/S0022-2275(20)40540-1","article-title":"Saposins: structure, function, distribution, and molecular genetics.","volume":"33","author":"Y Kishimoto","year":"1992","journal-title":"J. Lipid Res."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"668-674","DOI":"10.1016\/S0006-291X(05)80236-0","article-title":"Secretion of sphingolipid hydrolase activator precursor, prosaposin.","volume":"176","author":"T Hineno","year":"1991","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"117551","DOI":"10.1016\/j.biopha.2024.117551","article-title":"Dissecting the roles of prosaposin as an emerging therapeutic target for tumors and its underlying mechanisms.","volume":"180","author":"L Yan","year":"2024","journal-title":"Biomed. 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Protoc."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1344","DOI":"10.12688\/f1000research.141719.1","article-title":"YCharOS open antibody characterisation data: Lessons learned and progress made.","volume":"12","author":"M Biddle","year":"2023","journal-title":"F1000Res."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"8","DOI":"10.7554\/eLife.48363","article-title":"Implementation of an antibody characterization procedure and application to the major ALS\/FTD disease gene C9ORF72.","volume":"8","author":"C Laflamme","year":"2019","journal-title":"elife."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"977","DOI":"10.12688\/f1000research.124632.1","article-title":"Identification of highly specific antibodies for Serine\/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence.","volume":"11","author":"W Alshafie","year":"2022","journal-title":"F1000Res."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"12","DOI":"10.7554\/eLife.91645","article-title":"Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications.","volume":"12","author":"R Ayoubi","year":"2023","journal-title":"elife."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"D358-D367","DOI":"10.1093\/nar\/gkac927","article-title":"The Antibody Registry: ten years of registering antibodies.","volume":"51","author":"A Bandrowski","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"25-38","DOI":"10.7171\/jbt.18-2902-002","article-title":"The Cellosaurus, a Cell-Line Knowledge Resource.","volume":"29","author":"A Bairoch","year":"2018","journal-title":"J. Biomol. 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G-Can is supported by The Hilary & Galen Weston Foundation, Silverstein Foundation, and J. Sebastian van Berkom and Ghislaine Saucier. This work was also supported by the CQDM (by a grant from the Minist\u00e8re de l\u2019\u00c9conomie, de l\u2019Innovation et de l\u2019\u00c9nergie du Qu\u00e9bec). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}