{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:52:40Z","timestamp":1791895960524,"version":"4.3.4"},"reference-count":11,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>ARID1B encodes a core subunit of the SWI\/SNF (BAF) ATP-dependent chromatin-remodeling complex and plays a critical role in transcriptional regulation, cell differentiation, and development. Here we have characterized seven ARID1B commercial antibodies for western blot and immunoprecipitation by using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.<\/ns3:p>","DOI":"10.12688\/f1000research.179398.1","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:39:12Z","timestamp":1777379952000},"page":"631","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A guide to selecting high-performing antibodies for ARID1B (UniProt ID: Q8NFD5) for use in western blot and immunoprecipitation"],"prefix":"10.12688","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3728-3158","authenticated-orcid":false,"given":"Vera Ru\u00edz","family":"Mole\u00f3n","sequence":"first","affiliation":[{"name":"Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute Montreal Qu\u00e9bec McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4299-8281","authenticated-orcid":false,"given":"Sara Gonz\u00e1lez","family":"Bol\u00edvar","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute Montreal Qu\u00e9bec McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riham","family":"Ayoubi","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute Montreal Qu\u00e9bec McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7535-8718","authenticated-orcid":false,"given":"Vincent","family":"Francis","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute Montreal Qu\u00e9bec McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter S.","family":"McPherson","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute Montreal Qu\u00e9bec McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5906-025X","authenticated-orcid":false,"given":"Carl","family":"Laflamme","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute Montreal Qu\u00e9bec McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"ref1","first-page":"319-325","article-title":"Two related ARID family proteins are alternative subunits of human SWI\/SNF complexes.","volume":"383","author":"X Wang","year":"2004","journal-title":"Biochem. J."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"376-378","DOI":"10.1038\/ng.2219","article-title":"Mutations affecting components of the SWI\/SNF complex cause Coffin-Siris syndrome.","volume":"44","author":"Y Tsurusaki","year":"2012","journal-title":"Nat. Genet."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1721-1733","DOI":"10.1093\/hmg\/ddab134","article-title":"Downregulation of ARID1B, a tumor suppressor in the WNT subgroup medulloblastoma, activates multiple oncogenic signaling pathways.","volume":"30","author":"A Deogharkar","year":"2021","journal-title":"Hum. Mol. Genet."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1509-1545","DOI":"10.1038\/s41596-024-01095-8","article-title":"A consensus platform for antibody characterization.","volume":"20","author":"R Ayoubi","year":"2024","journal-title":"Nat. Protoc."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.12688\/f1000research.141719.1","article-title":"YCharOS open antibody characterisation data: Lessons learned and progress made F1000Research 2023.","volume":"12","author":"M Biddle","year":"2023","journal-title":"F1000Res."},{"key":"ref6","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.48363","article-title":"Implementation of an antibody characterization procedure and application to the major ALS\/FTD disease gene C9ORF72.","volume":"8","author":"C Laflamme","year":"2019","journal-title":"elife."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"977","DOI":"10.12688\/f1000research.124632.1","article-title":"Identification of highly specific antibodies for Serine\/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence.","volume":"11","author":"W Alshafie","year":"2022","journal-title":"F1000Res."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"12","DOI":"10.7554\/eLife.91645","article-title":"Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications.","volume":"12","author":"R Ayoubi","year":"2023","journal-title":"elife."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"D358-D367","DOI":"10.1093\/nar\/gkac927","article-title":"The Antibody Registry: ten years of registering antibodies.","volume":"51","author":"A Bandrowski","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"25-38","DOI":"10.7171\/jbt.18-2902-002","article-title":"The Cellosaurus, a Cell-Line Knowledge Resource.","volume":"29","author":"A Bairoch","year":"2018","journal-title":"J. Biomol. Tech."},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.19069583","volume":"2026","author":"V Francis","year":"2026","journal-title":"Zenodo."}],"container-title":["F1000Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/f1000research.com\/articles\/15-631\/v1\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/15-631\/v1\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/15-631\/v1\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:29:38Z","timestamp":1791894578000},"score":1,"resource":{"primary":{"URL":"https:\/\/f1000research.com\/articles\/15-631\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,28]]},"references-count":11,"URL":"https:\/\/doi.org\/10.12688\/f1000research.179398.1","relation":{"has-review":[{"id-type":"doi","id":"10.5256\/f1000research.197904.r518765","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.197904.r512463","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.197904.r518765","asserted-by":"object"},{"id-type":"doi","id":"10.5256\/f1000research.197904.r512463","asserted-by":"object"}]},"ISSN":["2046-1402"],"issn-type":[{"value":"2046-1402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,28]]},"assertion":[{"value":"Indexed","URL":"https:\/\/f1000research.com\/articles\/15-631\/v1#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.197904.r512463, Steve Jean, University of Sherbrooke, Sherbrooke, Qu\u00e9bec, 08 Sep 2026, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/15-631\/v1#referee-response-512463","order":0,"name":"referee-response-512463","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.197904.r518765, Deborah Moshinsky, Institute for Protein Innovation, Boston, Massachusetts, 26 Sep 2026, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/15-631\/v1#referee-response-518765","order":1,"name":"referee-response-518765","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported by the Terry Fox Foundation for Cancer Research (grant no. 1190-09). This work was also supported by a grant from the Quebec Consortium for Drug Discovery (CQDM), a grant from the Minist\u00e8re de l\u2019\u00c9conomie, de l\u2019Innovation et de l\u2019\u00c9nergie du Qu\u00e9bec. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}