{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T08:24:00Z","timestamp":1787646240274,"version":"build-2736575974"},"reference-count":11,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns5:p>\n                    DJ-1 is a multifunctional protein that plays a pivotal role in cellular protection against oxidative stress and neurodegeneration. Mutations in the\n                    <ns5:italic>PARK7<\/ns5:italic>\n                    gene are associated with early-onset familial Parkinson\u2019s disease. Here we have characterized sixteen DJ-1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.\n                  <\/ns5:p>","DOI":"10.12688\/f1000research.182633.1","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T04:24:08Z","timestamp":1782188648000},"page":"989","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A guide to selecting high-performing antibodies for DJ-1 (PARK7) (Q99497) for use in western blot, immunoprecipitation, and immunofluorescence"],"prefix":"10.12688","volume":"15","author":[{"given":"CongYao","family":"Zha","sequence":"first","affiliation":[{"name":"Montreal Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4611-6134","authenticated-orcid":false,"given":"Charles","family":"Alende","sequence":"additional","affiliation":[{"name":"Montreal Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Fotouhi","sequence":"additional","affiliation":[{"name":"Montreal Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4299-8281","authenticated-orcid":false,"given":"Sara Gonz\u00e1lez","family":"Bol\u00edvar","sequence":"additional","affiliation":[{"name":"Montreal Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riham","family":"Ayoubi","sequence":"additional","affiliation":[{"name":"Montreal Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7535-8718","authenticated-orcid":false,"given":"Vincent","family":"Francis","sequence":"additional","affiliation":[{"name":"Montreal Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter S.","family":"McPherson","sequence":"additional","affiliation":[{"name":"Montreal Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5906-025X","authenticated-orcid":false,"given":"Carl","family":"Laflamme","sequence":"additional","affiliation":[{"name":"Montreal Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"1-13","DOI":"10.1016\/j.neuroscience.2025.09.025","article-title":"DJ-1\/PARK7 in Parkinson's disease: mechanisms of pathogenesis and therapeutic potential.","volume":"587","author":"W Bao","year":"2025","journal-title":"Neuroscience."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"123-129","DOI":"10.1002\/jnr.21831","article-title":"Mitochondrial localization of DJ-1 leads to enhanced neuroprotection.","volume":"87","author":"E Junn","year":"2009","journal-title":"J. Neurosci. Res."},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.3390\/cells13040296","article-title":"Pathogenesis of DJ-1\/PARK7-Mediated Parkinson's Disease.","volume":"13","author":"L Skou","year":"2024","journal-title":"Cells."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1509-1545","DOI":"10.1038\/s41596-024-01095-8","article-title":"A consensus platform for antibody characterization.","volume":"20","author":"R Ayoubi","year":"2024","journal-title":"Nat. Protoc."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.12688\/f1000research.141719.1","article-title":"YCharOS open antibody characterisation data: Lessons learned and progress made.","volume":"12","author":"M Biddle","year":"2023","journal-title":"F1000Res."},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.48363","article-title":"Implementation of an antibody characterization procedure and application to the major ALS\/FTD disease gene C9ORF72.","volume":"8","author":"C Laflamme","year":"2019","journal-title":"elife."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"977","DOI":"10.12688\/f1000research.124632.1","article-title":"Identification of highly specific antibodies for Serine\/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence.","volume":"11","author":"W Alshafie","year":"2022","journal-title":"F1000Res."},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.91645","article-title":"Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications.","volume":"12","author":"R Ayoubi","year":"2023","journal-title":"elife."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"D358-D367","DOI":"10.1093\/nar\/gkac927","article-title":"The Antibody Registry: ten years of registering antibodies.","volume":"51","author":"A Bandrowski","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"25-38","DOI":"10.7171\/jbt.18-2902-002","article-title":"The Cellosaurus, a Cell-Line Knowledge Resource.","volume":"29","author":"A Bairoch","year":"2018","journal-title":"J. Biomol. Tech."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"100-106","DOI":"10.1038\/s41592-020-01018-x","article-title":"Cellpose: a generalist algorithm for cellular segmentation.","volume":"18","author":"C Stringer","year":"2021","journal-title":"Nat. Methods."}],"container-title":["F1000Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/f1000research.com\/articles\/15-989\/v1\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/15-989\/v1\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/15-989\/v1\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T07:19:22Z","timestamp":1787642362000},"score":1,"resource":{"primary":{"URL":"https:\/\/f1000research.com\/articles\/15-989\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,23]]},"references-count":11,"URL":"https:\/\/doi.org\/10.12688\/f1000research.182633.1","relation":{"has-review":[{"id-type":"doi","id":"10.5256\/f1000research.201594.r497512","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.201594.r508763","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.201594.r508763","asserted-by":"object"},{"id-type":"doi","id":"10.5256\/f1000research.201594.r497512","asserted-by":"object"}]},"ISSN":["2046-1402"],"issn-type":[{"value":"2046-1402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,23]]},"assertion":[{"value":"Indexed","URL":"https:\/\/f1000research.com\/articles\/15-989\/v1#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.201594.r497512, Jieya Shao, Washington University in St Louis, St. Louis, 15 Jul 2026, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/15-989\/v1#referee-response-497512","order":0,"name":"referee-response-497512","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.201594.r508763, Deborah Moshinsky, Institute for Protein Innovation, Boston, Massachusetts, 25 Aug 2026, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/15-989\/v1#referee-response-508763","order":1,"name":"referee-response-508763","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported by the Michael J. Fox Foundation for Parkinson\u2019s Research (MJFF) (grant no. 18331). This work was also supported by a grant from the Quebec Consortium for Drug Discovery (CQDM), a grant from the Minist\u00e8re de l\u2019\u00c9conomie, de l\u2019Innovation et de l\u2019\u00c9nergie du Qu\u00e9bec. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}