{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T05:18:00Z","timestamp":1788499080080,"version":"build-2803163510"},"reference-count":12,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>Syntenin-1 is the Syndecan-binding protein 1 and a PDZ domain\u2013containing adaptor protein that regulates diverse cellular processes through its interactions with transmembrane receptors, cytoskeletal components, and signaling molecules. Here we have characterized twelve Syntenin-1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.<\/ns3:p>","DOI":"10.12688\/f1000research.183125.1","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T11:09:08Z","timestamp":1782472148000},"page":"1021","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A guide to selecting high-performing antibodies for Syntenin-1 (O00560) for use in western blot, immunoprecipitation, and immunofluorescence"],"prefix":"10.12688","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3728-3158","authenticated-orcid":false,"given":"Vera Ru\u00edz","family":"Mole\u00f3n","sequence":"first","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4611-6134","authenticated-orcid":false,"given":"Charles","family":"Alende","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Fothouhi","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4299-8281","authenticated-orcid":false,"given":"Sara Gonz\u00e1lez","family":"Bol\u00edvar","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riham","family":"Ayoubi","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7535-8718","authenticated-orcid":false,"given":"Vincent","family":"Francis","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter S","family":"McPherson","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5906-025X","authenticated-orcid":false,"given":"Carl","family":"Laflamme","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"769-781","DOI":"10.1007\/s10555-020-09886-7","article-title":"MDA-9\/Syntenin\/SDCBP: new insights into a unique multifunctional scaffold protein.","volume":"39","author":"A Pradhan","year":"2020","journal-title":"Cancer Metastasis Rev."},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24119418","article-title":"The Multifunctional Protein Syntenin-1: Regulator of Exosome Biogenesis, Cellular Function, and Tumor Progression.","volume":"24","author":"K Lee","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"659-663","DOI":"10.1093\/gerona\/glab322","article-title":"Expression of Exosome Biogenesis Genes Is Differentially Altered by Aging in the Mouse and in the Human Brain During Alzheimer's Disease.","volume":"77","author":"D Lark","year":"2022","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1509-1545","DOI":"10.1038\/s41596-024-01095-8","article-title":"A consensus platform for antibody characterization.","volume":"20","author":"R Ayoubi","year":"2024","journal-title":"Nat. Protoc."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.12688\/f1000research.141719.1","article-title":"YCharOS open antibody characterisation data: Lessons learned and progress made.","volume":"12","author":"M Biddle","year":"2023","journal-title":"F1000Res."},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.48363","article-title":"Implementation of an antibody characterization procedure and application to the major ALS\/FTD disease gene C9ORF72.","volume":"8","author":"C Laflamme","year":"2019","journal-title":"elife"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"806-811","DOI":"10.1038\/35888","article-title":"Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.","volume":"391","author":"A Fire","year":"1998","journal-title":"Nature."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"48-57","DOI":"10.59566\/ijbs.2017.13048","article-title":"Molecular Mechanisms and Biological Functions of siRNA.","volume":"13","author":"H Dana","year":"2017","journal-title":"Int. J. Biomed. Sci."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"12","DOI":"10.7554\/eLife.91645","article-title":"Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications.","volume":"12","author":"R Ayoubi","year":"2023","journal-title":"elife."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"D358-D367","DOI":"10.1093\/nar\/gkac927","article-title":"The Antibody Registry: ten years of registering antibodies.","volume":"51","author":"A Bandrowski","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"25-38","DOI":"10.7171\/jbt.18-2902-002","article-title":"The Cellosaurus, a Cell-Line Knowledge Resource.","volume":"29","author":"A Bairoch","year":"2018","journal-title":"J. Biomol. Tech."},{"key":"ref12","doi-asserted-by":"publisher","first-page":"100-106","DOI":"10.1038\/s41592-020-01018-x","article-title":"Cellpose: a generalist algorithm for cellular segmentation.","volume":"18","author":"C Stringer","year":"2021","journal-title":"Nat. Methods."}],"container-title":["F1000Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/f1000research.com\/articles\/15-1021\/v1\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/15-1021\/v1\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/15-1021\/v1\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T04:39:18Z","timestamp":1788496758000},"score":1,"resource":{"primary":{"URL":"https:\/\/f1000research.com\/articles\/15-1021\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,26]]},"references-count":12,"URL":"https:\/\/doi.org\/10.12688\/f1000research.183125.1","relation":{"has-review":[{"id-type":"doi","id":"10.5256\/f1000research.202133.r508245","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.202133.r498830","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.202133.r498830","asserted-by":"object"},{"id-type":"doi","id":"10.5256\/f1000research.202133.r508245","asserted-by":"object"}]},"ISSN":["2046-1402"],"issn-type":[{"value":"2046-1402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,26]]},"assertion":[{"value":"Indexed","URL":"https:\/\/f1000research.com\/articles\/15-1021\/v1#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.202133.r498830, Jieya Shao, Washington University in St Louis, St. Louis, 22 Jul 2026, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/15-1021\/v1#referee-response-498830","order":0,"name":"referee-response-498830","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.202133.r508245, Karen Bowman, University of Leicester, Leicester, England, 04 Sep 2026, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/15-1021\/v1#referee-response-508245","order":1,"name":"referee-response-508245","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported in part by the Emory-Sage-SGC TaRget Enablement to Accelerate Therapy Development for Alzheimer\u2019s Disease (TREAT-AD) center established by the National Institute on Aging (grant no. U54AG065187). This work was also supported by a grant from the Quebec Consortium for Drug Discovery (CQDM), a grant from the Minist\u00e8re de l\u2019\u00c9conomie, de l\u2019Innovation et de l\u2019\u00c9nergie du Qu\u00e9bec. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}