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Here we have characterized fourteen Netrin-1 commercial antibodies for western blot and immunoprecipitation using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.<\/ns3:p>","DOI":"10.12688\/f1000research.184099.1","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T09:24:09Z","timestamp":1783675449000},"page":"1129","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A guide to selecting high-performing antibodies for Netrin-1 (UniProt ID: O95631) for use in western blot and immunoprecipitation"],"prefix":"10.12688","volume":"15","author":[{"given":"Riham","family":"Ayoubi","sequence":"first","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4299-8281","authenticated-orcid":false,"given":"Sara Gonz\u00e1lez","family":"Bol\u00edvar","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A","family":"Kahn","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7535-8718","authenticated-orcid":false,"given":"Vincent","family":"Francis","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter S.","family":"McPherson","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5906-025X","authenticated-orcid":false,"given":"Carl","family":"Laflamme","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"NeuroSGC\/YCharOS\/EDDU collaborative group and ABIF 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disease features.","volume":"40","author":"M Jasmin","year":"2021","journal-title":"EMBO J."},{"key":"ref8","article-title":"A consensus platform for antibody characterization.","author":"R Ayoubi","year":"2024","journal-title":"Nat Protoc."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"1344","DOI":"10.12688\/f1000research.141719.1","article-title":"YCharOS open antibody characterisation data: Lessons learned and progress made.","volume":"12","author":"M Biddle","year":"2023","journal-title":"F1000Res."},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.48363","article-title":"Implementation of an antibody characterization procedure and application to the major ALS\/FTD disease gene C9ORF72.","volume":"8","author":"C Laflamme","year":"2019","journal-title":"Elife."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"977","DOI":"10.12688\/f1000research.124632.1","article-title":"Identification of highly specific antibodies for 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status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.203211.r512302, Karen Bowman, University of Leicester, Leicester, England, 11 Sep 2026, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/15-1129\/v1#referee-response-512302","order":0,"name":"referee-response-512302","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.203211.r512301, Jieya Shao, Washington University in St Louis, St. Louis, 15 Sep 2026, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/15-1129\/v1#referee-response-512301","order":1,"name":"referee-response-512301","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported by a grant from the CQDM (by a grant from the Minist\u00e8re de l\u2019\u00c9conomie, de l\u2019Innovation et de l\u2019\u00c9nergie du Qu\u00e9bec).The Government of Canada through Genome Canada, Genome Quebec, and Ontario Genomics (grant no. OGI-210). RA is supported by a Mitacs fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}