{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T07:18:16Z","timestamp":1788160696565,"version":"build-2803163510"},"reference-count":8,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>Kinesin Family Member 5A (KIF5A) is an important protein for anterograde cellular transport. We characterized ten commercially available research antibodies against KIF5A for use in western blot, immunoprecipitation, and immunofluorescence using a standardized workflow. Antibody performance was assessed by comparing signal in a wild-type cell line and its corresponding knockout derivative. This work is part of a broader collaborative public-good initiative to improve biomedical research by systematically evaluating commercial antibodies against human proteins and openly sharing the data as a resource for the scientific community. We encourage readers to use this report as a guide for selecting antibodies best suited to their specific applications.<\/ns3:p>","DOI":"10.12688\/f1000research.184178.1","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T06:19:07Z","timestamp":1784701147000},"page":"1030","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["A guide to selecting high-performing renewable antibodies for KIF5A (Q12840) across western blot, immunoprecipitation, and immunofluorescence"],"prefix":"10.12688","volume":"15","author":[{"given":"Riham","family":"Ayoubi","sequence":"first","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4611-6134","authenticated-orcid":false,"given":"Charles","family":"Alende","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Fothouhi","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4299-8281","authenticated-orcid":false,"given":"Sara Gonz\u00e1lez","family":"Bol\u00edvar","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7535-8718","authenticated-orcid":false,"given":"Vincent","family":"Francis","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter S.","family":"McPherson","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5906-025X","authenticated-orcid":false,"given":"Carl","family":"Laflamme","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurosurgery Montreal Structural Genomics Consortium, The Montreal Neurological Institute, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"NeuroSGC\/YCharOS\/EDDU collaborative group","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1186\/s12964-025-02277-x","article-title":"One gene, many phenotypes: the role of KIF5A in neurodegenerative and neurodevelopmental diseases.","volume":"23","author":"M Cozzi","year":"2025","journal-title":"Cell Commun. 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Methods."}],"container-title":["F1000Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/f1000research.com\/articles\/15-1030\/v1\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/15-1030\/v1\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/15-1030\/v1\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T07:04:11Z","timestamp":1788159851000},"score":1,"resource":{"primary":{"URL":"https:\/\/f1000research.com\/articles\/15-1030\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,30]]},"references-count":8,"URL":"https:\/\/doi.org\/10.12688\/f1000research.184178.1","relation":{"has-review":[{"id-type":"doi","id":"10.5256\/f1000research.203297.r512728","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.203297.r501651","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.203297.r512728","asserted-by":"object"}]},"ISSN":["2046-1402"],"issn-type":[{"value":"2046-1402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,30]]},"assertion":[{"value":"Indexed","URL":"https:\/\/f1000research.com\/articles\/15-1030\/v1#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.203297.r501651, Karen Bowman, University of Leicester, Leicester, England, 22 Jul 2026, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/15-1030\/v1#referee-response-501651","order":0,"name":"referee-response-501651","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.203297.r512728, Deborah Moshinsky, Institute for Protein Innovation, Boston, Massachusetts, 31 Aug 2026, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/15-1030\/v1#referee-response-512728","order":1,"name":"referee-response-512728","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported in part by a grant from the ALS-Reproducible Antibody Platform (ALS-RAP). ALS-RAP was created as a public-private partnership by three leading ALS charities: the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada. This work was also supported by a grant from the Quebec Consortium for Drug Discovery (CQDM), a grant from the Minist\u00e8re de l\u2019\u00c9conomie, de l\u2019Innovation et de l\u2019\u00c9nergie du Qu\u00e9bec.\n\nThank you to the Structural Genomics Consortium, a registered charity (no. 1097737), for your support on this project. The Structural Genomics Consortium receives funding from Bayer AG, Boehringer Ingelheim, Bristol-Myers Squibb, Genentech, Genome Canada through Ontario Genomics Institute (grant no. OGI-196), the EU and EFPIA through the Innovative Medicines Initiative 2 Joint Undertaking (EUbOPEN grant no. 875510), Janssen, Merck KGaA (also known as EMD in Canada and the United States), Pfizer and Takeda. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}