{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:15:57Z","timestamp":1777421757547,"version":"3.51.4"},"reference-count":38,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2019,11,11]],"date-time":"2019-11-11T00:00:00Z","timestamp":1573430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Background:<\/ns4:bold>\n                    Recent efforts have described an immunogenic component to the pathobiology of Alzheimer\u2019s disease (AD) and Parkinson\u2019s disease (PD). However, current methods of studying fluid autoantibodies, such as enzyme-linked immunosorbent assays and immunohistochemistry, are hypothesis-driven and not optimal for discovering new autoantibody biomarkers by proteome-wide screening. Recently, we developed a general mass spectrometry-based approach to identify tissue-specific autoantibodies in serum, at a proteome-wide level. In this study, we adapted the method to explore novel autoantibody biomarkers in the cerebrospinal fluid (CSF) of AD and PD patients.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods:<\/ns4:bold>\n                    CSF samples were obtained from 10 headache control individuals, 10 AD patients and 10 PD patients. Antibodies present in the CSF were isolated by immobilization to protein-G magnetic beads. These antibodies were incubated with a brain tissue extract, prepared from frontal cortex, pons, cerebellum and brain stem. Protein antigens captured by the protein-G magnetic bead-bound antibodies were digested with trypsin and analyzed using mass spectrometry. Autoantibody candidates were selected by 1) detection in one or less individuals of the control group and 2) identification in at least half of the patient groups.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Results:<\/ns4:bold>\n                    There were 16 putative autoantibody biomarkers selected from the AD group. Glia-derived nexin autoantibody was detected in eight of ten AD patients and was absent in the control group. Other AD pathology-related targets were also identified, such as actin-interaction protein, quinone oxidoreductase, sushi repeat-containing protein, metalloproteinase inhibitor 2, IP3 receptor 1 and sarcoplasmic\/endoplasmic reticulum calcium ATPase 2. An additional eleven autoantibody targets were also identified in the present experiment, although their link to AD is not clear. No autoantibodies in the PD group satisfied our selection criteria.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Conclusion:<\/ns4:bold>\n                    Our unbiased mass spectrometry method was able to detect new putative CSF autoantibody biomarkers of AD. Further investigation into the involvement of humoral autoimmunity in AD and PD pathobiology may be warranted.\n                  <\/ns4:p>","DOI":"10.12688\/f1000research.21140.1","type":"journal-article","created":{"date-parts":[[2019,11,11]],"date-time":"2019-11-11T07:10:08Z","timestamp":1573456208000},"page":"1900","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Putative autoantibodies in the cerebrospinal fluid of Alzheimer\u2019s disease patients"],"prefix":"10.12688","volume":"8","author":[{"given":"Bryant","family":"Lim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magda","family":"Tsolaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ihor","family":"Batruch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Anastasiou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonis","family":"Frontistis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7907-4576","authenticated-orcid":false,"given":"Ioannis","family":"Prassas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1589-820X","authenticated-orcid":false,"given":"Eleftherios P.","family":"Diamandis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2019,11,11]]},"reference":[{"key":"ref-1","doi-asserted-by":"publisher","first-page":"836-847","DOI":"10.1002\/mds.26656","article-title":"Cerebrospinal fluid biomarkers in Alzheimer's and Parkinson's diseases-From pathophysiology to clinical practice.","volume":"31","author":"K Blennow","year":"2016","journal-title":"Mov Disord."},{"key":"ref-2","doi-asserted-by":"publisher","first-page":"405-411","DOI":"10.1097\/WCO.0000000000000346","article-title":"Cognitive interventions in Alzheimer's and Parkinson's diseases: emerging mechanisms and role of imaging.","volume":"29","author":"P Vemuri","year":"2016","journal-title":"Curr Opin Neurol."},{"key":"ref-3","doi-asserted-by":"publisher","first-page":"115-120","DOI":"10.1016\/j.coi.2019.07.004","article-title":"Roles for the adaptive immune system in Parkinson's and 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Booth, University of Ottawa, Ottawa, ON, Canada, 05 Feb 2020, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/8-1900\/v1#referee-response-58124","order":1,"name":"referee-response-58124","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.23271.r58125, Stefan Holdenrieder, German Heart Centre, Technical University of Munich (TUM), Munich, Germany, 07 Feb 2020, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/8-1900\/v1#referee-response-58125","order":2,"name":"referee-response-58125","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"The author(s) declared that no grants were involved in supporting this work","order":3,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}