{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:09:45Z","timestamp":1772168985863,"version":"3.50.1"},"update-to":[{"DOI":"10.12688\/f1000research.21211.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T00:00:00Z","timestamp":1591228800000}}],"reference-count":29,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T00:00:00Z","timestamp":1591228800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009509","name":"Kementerian Riset Teknologi Dan Pendidikan Tinggi Republik Indonesia","doi-asserted-by":"publisher","award":["2853\/UN1.DITLIT\/DIT-LIT\/LT\/2019"],"award-info":[{"award-number":["2853\/UN1.DITLIT\/DIT-LIT\/LT\/2019"]}],"id":[{"id":"10.13039\/501100009509","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Background:<\/ns4:bold>\n                    Orthodontic treatment to improve aesthetics and for health reasons is performed in children and adults. Elderly individuals have low levels of estrogen, this results in alveolar bone resorption being greater than alveolar bone apposition. Isoflavones present in soybeans may be able to improve the remodeling process through the induction of osteoblastogenesis by increasing transforming growth factor-\u03b21 (TGF-\u03b21) levels. This study aimed to assess the comparative effect of soybean genistein isoflavone to TGF-\u03b21 during orthodontic tooth movement among juvenile and adult rabbits.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Method<\/ns4:bold>\n                    <ns4:bold>s<\/ns4:bold>\n                    : In this study, 12 healthy female rabbits were used. Subjects were divided into four groups (n=3); YG group (young rabbits), YGI group (young rabbits + isoflavones genistein), OG group (old rabbits), and OGI group (old rabbits + isoflavones genistein). Two lower incisors of the rabbit were moved distally using an orthodontic force (50 grams force) delivered by an open coil spring, which was inserted between two brackets. During active movements, the genistein isoflavones were given from the initial installation of the device until days 21, at a dose of 1.2 mg\/kg BW once a day. Measurement of TGF-\u03b2 levels were performed on days 1, 7, 14, 21 after appliance installation. TGF-\u03b21 expression was analyzed using enzyme-linked immunosorbent assay (ELISA) and the optical density (OD) of the sample quantifed using a standard curve. The data obtained were analyzed using one-way Anova followed by Tukey HSD test.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Results:<\/ns4:bold>\n                    The TGF-\u03b21 levels were found to highest in the YGI group, and the TGF-\u03b2 levels were significantly lower in the OG group (\n                    <ns4:italic>p<\/ns4:italic>\n                    &lt;0.05). ELISA analysis also revealed that TGF-\u03b21 levels of the OGI group were significantly higher when compared with the OG group (\n                    <ns4:italic>p<\/ns4:italic>\n                    &lt;0.05).\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Conclusion:<\/ns4:bold>\n                    The administration of soybean genistein isoflavones could improve TGF-\u03b21 levels in old rabbit\u2019s during active orthodontic tooth movement.\n                  <\/ns4:p>","DOI":"10.12688\/f1000research.21211.2","type":"journal-article","created":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T10:05:08Z","timestamp":1591265108000},"page":"2074","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Different effects of soybean isoflavone genistein on transforming growth factor levels during orthodontic tooth movement among young and old rabbits"],"prefix":"10.12688","volume":"8","author":[{"given":"Verastuti","family":"Indriasari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7497-0849","authenticated-orcid":false,"given":"Sri","family":"Suparwitri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christnawati","family":"Christnawati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1402-4543","authenticated-orcid":false,"given":"Ananto Ali","family":"Alhasyimi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2020,6,4]]},"reference":[{"key":"ref-1","doi-asserted-by":"publisher","first-page":"58-64","DOI":"10.1590\/2177-6709.23.5.058-064.oar","article-title":"Effect of mangosteen peel extract as an 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Indriasari","year":"2019","journal-title":"figshare."}],"container-title":["F1000Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/f1000research.com\/articles\/8-2074\/v2\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/8-2074\/v2\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/8-2074\/v2\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T04:40:26Z","timestamp":1591936826000},"score":1,"resource":{"primary":{"URL":"https:\/\/f1000research.com\/articles\/8-2074\/v2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,4]]},"references-count":29,"URL":"https:\/\/doi.org\/10.12688\/f1000research.21211.2","relation":{"has-review":[{"id-type":"doi","id":"10.5256\/f1000research.27030.r64307","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.27030.r64307","asserted-by":"object"}]},"ISSN":["2046-1402"],"issn-type":[{"value":"2046-1402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,4]]},"assertion":[{"value":"Indexed","URL":"https:\/\/f1000research.com\/articles\/8-2074\/v2#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.23351.r58276, Ida Bagus Narmada, Alexander Patera Nugraha, Department of Orthodontics, Faculty of Dental Medicine, Airlangga\u00a0University, Surabaya, Indonesia, 20 Jan 2020, version 1, 1 approved, 1 approved with reservations","URL":"https:\/\/f1000research.com\/articles\/8-2074\/v1#referee-response-58276","order":0,"name":"referee-response-58276","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Ananto Ali Alhasyimi<\/b>; \n<i>Posted: 29 May 2020<\/i>; Dear Prof \n<b>Narmada and Dr Nugraha<\/b> Thank you for your kind assistance in reviewing our manuscript and providing us with valuable advice for the manuscript and our future work. We have been working throughout the review. We have added this information in the present manuscript.&nbsp;We thank you again for the valuable insight.","URL":"https:\/\/f1000research.com\/articles\/8-2074\/v2#referee-comment-5559","order":1,"name":"referee-comment-5559","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.27030.r64307, Lidia Audrey Rocha Valadas, Postgraduate Program in Development and Technological Innovation in Drugs, School of Pharmacy, Federal University of Cear\u00e1, Fortaleza, Brazil, 12 Jun 2020, version 2, indexed","URL":"https:\/\/f1000research.com\/articles\/8-2074\/v2#referee-response-64307","order":2,"name":"referee-response-64307","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This study project is entirely financed by the Ministry of Research, Technology and Higher Education,of the Indonesian Government [2853\/UN1.DITLIT\/DIT-LIT\/LT\/2019]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":3,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}