{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T20:01:13Z","timestamp":1793908873537,"version":"4.8.0"},"reference-count":39,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2014,2,19]],"date-time":"2014-02-19T00:00:00Z","timestamp":1392768000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>\n                    Fluorination has been widely used in chemical synthesis, but is rare in nature. The only known biological fluorination scope is represented by the\n                    <ns4:italic>fl<\/ns4:italic>\n                    pathway from\n                    <ns4:italic>Streptomyces cattleya<\/ns4:italic>\n                    that produces fluoroacetate (FAc) and 4-fluorothreonine (4-FT). Here we report the identification of a novel pathway for FAc and 4-FT biosynthesis from the actinomycetoma-causing pathogen\n                    <ns4:italic>Nocardia brasiliensis<\/ns4:italic>\n                    ATCC 700358. The new pathway shares overall conservation with the\n                    <ns4:italic>fl<\/ns4:italic>\n                    pathway in\n                    <ns4:italic>S. cattleya<\/ns4:italic>\n                    . Biochemical characterization of the conserved domains revealed a novel fluorinase NobA that can biosynthesize 5\u2019-fluoro-5\u2019-deoxyadenosine (5\u2019-FDA) from inorganic fluoride and\n                    <ns4:italic>S<\/ns4:italic>\n                    -adenosyl-l-methionine (SAM). The NobA shows similar halide specificity and characteristics to the fluorination enzyme FlA of the\n                    <ns4:italic>fl<\/ns4:italic>\n                    pathway. Kinetic parameters for fluoride (\n                    <ns4:italic>\n                      K\n                      <ns4:sub>m<\/ns4:sub>\n                    <\/ns4:italic>\n                    4153 \u03bcM,\n                    <ns4:italic>\n                      k\n                      <ns4:sub>cat<\/ns4:sub>\n                    <\/ns4:italic>\n                    0.073 min\n                    <ns4:sup>-1<\/ns4:sup>\n                    ) and SAM (\n                    <ns4:italic>\n                      K\n                      <ns4:sub>m<\/ns4:sub>\n                    <\/ns4:italic>\n                    416 \u03bcM,\n                    <ns4:italic>\n                      k\n                      <ns4:sub>cat<\/ns4:sub>\n                    <\/ns4:italic>\n                    0.139 min\n                    <ns4:sup>-1<\/ns4:sup>\n                    ) have been determined, revealing that NobA is slightly (2.3 fold) slower than FlA. Upon sequence comparison, we finally identified a distinct loop region in the fluorinases that probably accounts for the disparity of fluorination activity.\n                  <\/ns4:p>","DOI":"10.12688\/f1000research.3-61.v1","type":"journal-article","created":{"date-parts":[[2014,2,19]],"date-time":"2014-02-19T07:38:51Z","timestamp":1392795531000},"page":"61","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":26,"title":["Characterization of a SAM-dependent fluorinase from a latent biosynthetic pathway for fluoroacetate and 4-fluorothreonine formation in Nocardia brasiliensis"],"prefix":"10.12688","volume":"3","author":[{"given":"Yaya","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8590-0574","authenticated-orcid":false,"given":"Zixin","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xudong","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2014,2,19]]},"reference":[{"key":"ref-1","doi-asserted-by":"publisher","first-page":"3496-3508","DOI":"10.1039\/c0cs00221f","article-title":"Organic fluorine compounds: A great opportunity for enhanced materials properties.","volume":"40","author":"R Berger","year":"2011","journal-title":"Chem Soc Rev."},{"key":"ref-2","doi-asserted-by":"publisher","DOI":"10.1021\/cr4002879","article-title":"Fluorine in pharmaceutical industry: Fluorine-containing drugs introduced to the market in the last decade (2001\u20132011).","author":"J Wang","year":"2013","journal-title":"Chem Rev."},{"key":"ref-3","doi-asserted-by":"publisher","first-page":"1881-1886","DOI":"10.1126\/science.1131943","article-title":"Fluorine in pharmaceuticals: Looking beyond intuition.","volume":"317","author":"K M\u00fcller","year":"2007","journal-title":"Science."},{"key":"ref-4","doi-asserted-by":"publisher","first-page":"308-319","DOI":"10.1039\/b711844a","article-title":"Understanding organofluorine chemistry. 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status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.3671.r3786, Changsheng Zhang, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China, 05 Mar 2014, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/3-61\/v1#referee-response-3786","order":0,"name":"referee-response-3786","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.3671.r4207, Cormac Murphy, Center for Synthesis and Chemical Biology, University College Dublin, Dublin, Ireland, 24 Mar 2014, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/3-61\/v1#referee-response-4207","order":1,"name":"referee-response-4207","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported in part by the National Natural Science Foundation of China (31270119, 31322002) and the program for New Century Excellent Talents in University (NCET-12-0423).","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Data associated with the article are available under the terms of the Creative Commons Zero \"No rights reserved\" data waiver (CC0 1.0 Public domain dedication).","order":0,"name":"copyright-info","label":"Copyright"}]}}