{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T12:20:59Z","timestamp":1779106859878,"version":"3.51.4"},"reference-count":37,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2014,6,2]],"date-time":"2014-06-02T00:00:00Z","timestamp":1401667200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>Genetic interaction networks that underlie most human diseases are highly complex and poorly defined. Better-defined networks will allow identification of a greater number of therapeutic targets.<\/ns4:p>\n                  <ns4:p>\n                    Here we introduce our\n                    <ns4:underline>Y<\/ns4:underline>\n                    east\n                    <ns4:underline>A<\/ns4:underline>\n                    ugmented\n                    <ns4:underline>N<\/ns4:underline>\n                    etwork\n                    <ns4:underline>A<\/ns4:underline>\n                    nalysis (YANA) approach and test it with the X-linked spinal muscular atrophy (SMA) disease gene\n                    <ns4:italic>UBA1.\u00a0<\/ns4:italic>\n                    First, we express\n                    <ns4:italic>UBA1<\/ns4:italic>\n                    and a mutant variant in fission yeast and use high-throughput methods to identify fission yeast genetic modifiers of\n                    <ns4:italic>UBA1<\/ns4:italic>\n                    . Second, we analyze available protein-protein interaction network databases in both fission yeast and human to construct\n                    <ns4:italic>UBA1<\/ns4:italic>\n                    genetic networks. Third, from these networks we identified potential therapeutic targets for SMA. Finally, we validate one of these targets in a vertebrate (zebrafish) SMA model. This study demonstrates the power of combining synthetic and chemical genetics with a simple model system to identify human disease gene networks that can be exploited for treating human diseases.\n                  <\/ns4:p>","DOI":"10.12688\/f1000research.4188.1","type":"journal-article","created":{"date-parts":[[2014,6,17]],"date-time":"2014-06-17T17:25:20Z","timestamp":1403025920000},"page":"121","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Yeast Augmented Network Analysis (YANA): a new systems approach to identify therapeutic targets for human genetic diseases"],"prefix":"10.12688","volume":"3","author":[{"given":"David 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Wiley","journal-title":"F1000Research."}],"container-title":["F1000Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/f1000research.com\/articles\/3-121\/v1\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/3-121\/v1\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/f1000research.com\/articles\/3-121\/v1\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T12:18:31Z","timestamp":1649420311000},"score":1,"resource":{"primary":{"URL":"https:\/\/f1000research.com\/articles\/3-121\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,2]]},"references-count":37,"URL":"https:\/\/doi.org\/10.12688\/f1000research.4188.1","relation":{"has-review":[{"id-type":"doi","id":"10.5256\/f1000research.4484.r5211","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.4484.r4970","asserted-by":"subject"},{"id-type":"doi","id":"10.5256\/f1000research.4484.r4970","asserted-by":"object"},{"id-type":"doi","id":"10.5256\/f1000research.4484.r5211","asserted-by":"object"}]},"ISSN":["2046-1402"],"issn-type":[{"value":"2046-1402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,2]]},"assertion":[{"value":"Indexed","URL":"https:\/\/f1000research.com\/articles\/3-121\/v1#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.4484.r4970, Corey Nislow, Department of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, Canada, 05 Jun 2014, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/3-121\/v1#referee-response-4970","order":0,"name":"referee-response-4970","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.4484.r5211, Jasper Rine, Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA, 23 Jun 2014, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/3-121\/v1#referee-response-5211","order":1,"name":"referee-response-5211","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported by a grant from the Muscular Dystrophy Association (MDA186435). (Christine Beattie grant support RO1NS050414 (C.E.B.) with additional support from P30NS045758). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Data associated with the article are available under the terms of the Creative Commons Zero \"No rights reserved\" data waiver (CC0 1.0 Public domain dedication).","order":0,"name":"copyright-info","label":"Copyright"}]}}