{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:08:56Z","timestamp":1772168936159,"version":"3.50.1"},"update-to":[{"DOI":"10.12688\/f1000research.53221.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T00:00:00Z","timestamp":1636934400000}},{"DOI":"10.12688\/f1000research.53221.2","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T00:00:00Z","timestamp":1636934400000}}],"reference-count":20,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T00:00:00Z","timestamp":1636934400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>\n                    <ns3:bold>Background\u00a0<\/ns3:bold>\n                  <\/ns3:p>\n                  <ns3:p>\n                    The presence of Extended-spectrum \u03b2-lactamase\u00a0positive bacteria\u00a0in hospital setting is an aggravating influential factor for hospitalized patients, and its consequences may be hazardous. Therefore, there is a need for rapid detection methods for newly emerging drug-resistant bacteria.\u00a0This study\u00a0was\u00a0aimed\u00a0at\u00a0the molecular characterization of Extended-spectrum \u03b2-lactamase -positive\u00a0\n                    <ns3:italic>Klebsiella pneumoniae<\/ns3:italic>\n                    isolates recovered from the patients of a teaching hospital in Sindh, Pakistan.\u00a0\u00a0\n                  <\/ns3:p>\n                  <ns3:p>\n                    <ns3:bold>Methods\u00a0<\/ns3:bold>\n                  <\/ns3:p>\n                  <ns3:p>\n                    A total of 513\u00a0\n                    <ns3:italic>K. pneumoniae<\/ns3:italic>\n                    \u00a0isolates\u00a0were\u00a0obtained\u00a0from\u00a0various\u00a0clinical samples\u00a0during June 2019\u00a0to May 2020. The collected\u00a0isolates\u00a0were investigated for antimicrobial susceptibility\u00a0(antibiogram),\u00a0and\u00a0PCR\u00a0and DNA sequencing\u00a0were performed to analyse\u00a0the\u00a0ESBL genes.\u00a0\u00a0\n                  <\/ns3:p>\n                  <ns3:p>\n                    <ns3:bold>Results\u00a0<\/ns3:bold>\n                  <\/ns3:p>\n                  <ns3:p>Among\u00a0the\u00a0513\u00a0isolates,\u00a0as many as\u00a0359 (69.9%)\u00a0were\u00a0Extended-spectrum \u03b2-lactamase\u00a0producers and 87.5% were multi-drug resistant,\u00a0while\u00a0none had\u00a0resistance to imipenem. PCR scored\u00a03%\u00a0blaTEM, 3%\u00a0blaSHV, and 60%\u00a0blaCTX-M-15 genes for the tested isolates.\u00a0\u00a0<\/ns3:p>\n                  <ns3:p>\n                    <ns3:bold>Conclusion\u00a0<\/ns3:bold>\n                  <\/ns3:p>\n                  <ns3:p>The study showed that CTX-M-15\u00a0was\u00a0the major prevalent Extended-spectrum \u03b2-lactamase type\u00a0among the isolates.\u00a0Additionally, all\u00a0the\u00a0isolates were susceptible to carbapenems. Screening and\u00a0detection\u00a0of Extended-spectrum \u03b2-lactamase tests are necessary among all isolates from the\u00a0enterobacteriaceae\u00a0family in routine microbiology laboratory to prevent associated nosocomial infections. A larger study is essential to understand molecular epidemiology of Extended-spectrum \u03b2-lactamase producing organisms to minimize morbidities\u00a0due to\u00a0these\u00a0multidrug resistant organisms.<\/ns3:p>","DOI":"10.12688\/f1000research.53221.3","type":"journal-article","created":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T06:01:32Z","timestamp":1636956092000},"page":"444","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular Epidemiological Surveillance of CTX-M-15-producing Klebsiella pneumoniae from the patients of a teaching hospital in Sindh, Pakistan."],"prefix":"10.12688","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2814-8205","authenticated-orcid":false,"given":"Muzaheed","family":"Muzaheed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naveed","family":"Sattar Shaikh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9367-2292","authenticated-orcid":false,"given":"Saeed","family":"Sattar Shaikh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadananda","family":"Acharya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shajiya","family":"Sarwar Moosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5348-1010","authenticated-orcid":false,"given":"Mohammad","family":"Habeeb Shaikh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faisal","family":"M. 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