{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:05:52Z","timestamp":1773360352292,"version":"3.50.1"},"reference-count":23,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T00:00:00Z","timestamp":1626307200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Cartagena","award":["078 and 095\/2019"],"award-info":[{"award-number":["078 and 095\/2019"]}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns3:p>\n                    <ns3:bold>Background:<\/ns3:bold>\n                    Worldwide, chicken meat is widely consumed due to its low cost, high nutritional value and non-interference with religious or cultural beliefs. However, during animal husbandry chickens are exposed to many chemical substances, including tetracyclines and \u03b2-lactams, which are used to prevent and cure several infections. Some residues of these compounds may bioaccumulate and be present in chicken meat after slaughtering, promoting oxidative reactions.\n                  <\/ns3:p>\n                  <ns3:p>\n                    <ns3:bold>Methods:<\/ns3:bold>\n                    In order to evaluate\n                    <ns3:italic>in vitro<\/ns3:italic>\n                    carbonylation induced by tetracyclines and \u03b2-lactams residues, a proteomic approach was used. For this, chicken muscle was individually contaminated with tetracyclines (tetracycline, chlortetracycline, oxytetracycline, and doxycycline) and \u03b2-lactams (ampicillin, benzathine penicillin, dicloxacillin and oxacillin) at 0.5, 1.0 and 1.5 times their maximum residue level (MRL). Then, sarcoplasmic, myofibrillar and insoluble proteins were extracted and their content were measured using the Bradford method. Protein carbonylation was measured using the 2,4-Dinitrophenylhydrazine alkaline method.\n                  <\/ns3:p>\n                  <ns3:p>\n                    <ns3:bold>Results:<\/ns3:bold>\n                    Residues of tetracyclines and \u03b2-lactams induced\n                    <ns3:italic>in vitro<\/ns3:italic>\n                    carbonylation on sarcoplasmic, myofibrillar and insoluble proteins even at 0.5MRL concentrations (\n                    <ns3:italic>p&lt;0.05<\/ns3:italic>\n                    ). When comparing the carbonylation induced by both antibiotics no differences were found (\n                    <ns3:italic>p&gt;0.05<\/ns3:italic>\n                    ). Variables such as the partition coefficient (log P) and the concentration of these antibiotics showed a high correlation with the oxidative capacity of tetracyclines and \u03b2-lactams on chicken breast proteins.\n                  <\/ns3:p>\n                  <ns3:p>\n                    <ns3:bold>Conclusions<\/ns3:bold>\n                    : This study shows that the presence of tetracyclines and \u03b2-lactams residues at MRLs concentrations promotes\n                    <ns3:italic>in vitro<\/ns3:italic>\n                    carbonylation on chicken breast proteins. Our results provide important insights about the impact of antibiotics on the integrity of meat proteins intended for human consumption.\n                  <\/ns3:p>","DOI":"10.12688\/f1000research.53863.1","type":"journal-article","created":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T06:25:16Z","timestamp":1626330316000},"page":"575","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Residues of tetracyclines and \u03b2-lactams antibiotics induce carbonylation of chicken\u00a0breast"],"prefix":"10.12688","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8828-3735","authenticated-orcid":false,"given":"Johana","family":"Marquez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1843-4782","authenticated-orcid":false,"given":"Albeiro","family":"Marrugo Padilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dar\u00edo","family":"M\u00e9ndez Cuadro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erika","family":"Rodr\u00edguez Cavallo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2021,7,15]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"140-145","DOI":"10.14202\/vetworld.2020.61-67","article-title":"Screening of Antibiotic Residues in Chicken Meat in Bangladesh by Thin Layer Chromatography.","volume":"5","author":"Y Sarker","year":"2018","journal-title":"J Adv Vet Anim Res."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"1-11","DOI":"10.3390\/ani10061056","article-title":"Assessing Antibiotic Residues in Poultry Eggs from Backyard Production Systems in Chile, First Approach to a Non-Addressed Issue in Farm Animals.","volume":"10","author":"J Cornejo","year":"2020","journal-title":"Animals."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"126922","DOI":"10.1016\/j.foodchem.2020.126922","article-title":"In Vitro Oxidation Promoted by Chlorpyrifos Residues on Myosin and Chicken Breast Proteins.","volume":"326","author":"J M\u00e1rquez-L\u00e1zaro","year":"2020","journal-title":"Food Chem."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"104708","DOI":"10.1016\/j.idairyj.2020.104708","article-title":"Combined Tetracycline and Pyrethroid Residues Increases Protein Carbonylation in Bovine Milk.","author":"A Padilla","year":"2020","journal-title":"Int Dairy J."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"1-30","DOI":"10.3390\/antiox9121212","article-title":"Protein Carbonylation and Lipid Peroxidation in Hematological Malignancies.","volume":"9","author":"A Rodr\u00edguez-Garc\u00eda","year":"2020","journal-title":"Antioxidants"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"1293-1302","DOI":"10.1515\/chem-2015-0147","article-title":"Carbonyl and Sulfhydryl Groups of Chicken Meat Proteins after Dietary Modulation with Selenium.","volume":"13","author":"M Korzeniowska","year":"2015","journal-title":"Open Chem."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"1368-1378","DOI":"10.3382\/ps\/pev094","article-title":"Oxidative Damage to Poultry: From Farm to Fork.","volume":"94","author":"M Est\u00e9vez","year":"2015","journal-title":"Poult. 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status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.5256\/f1000research.57287.r102491, Ma\u0142gorzata Korzeniowska, Department of Functional Food Products Development, Faculty of Biotechnology and Food Sciences, Wroc\u0142aw University of Environmental and Life Sciences, Wroc\u0142aw, Poland, 18 Feb 2022, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/10-575\/v1#referee-response-102491","order":0,"name":"referee-response-102491","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.57287.r121739, Chenliang Li, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA-CSIC), Valencia, Spain, 11 Mar 2022, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/10-575\/v1#referee-response-121739","order":1,"name":"referee-response-121739","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"The authors express their gratitude to the University of Cartagena for grants 078 and 095\/2019 and Corporaci\u00f3n Universitaria Rafael N\u00fa\u00f1ez. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}