{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:11:08Z","timestamp":1772169068846,"version":"3.50.1"},"reference-count":41,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This work was supported with a stipend to Mona Ali Mahmoud Assar by the Egyptian cultural affairs and missions\u2019 sector and ministry of higher education"},{"name":"European Union and NRW government","award":["(NRW-EU.Ziel2, (EFRE), 005-0908-0112)"],"award-info":[{"award-number":["(NRW-EU.Ziel2, (EFRE), 005-0908-0112)"]}]}],"content-domain":{"domain":["f1000research.com"],"crossmark-restriction":false},"short-container-title":["F1000Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Abstract<\/ns4:bold>\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Background:<\/ns4:bold>\n                    Asparaginases are common chemotherapeutic agents used for the treatment of acute lymphoblastic leukemia as a single or combinational therapy. Accompanying hepatotoxicity makes its use in elderly patients with pre-conditions, as obesity or other hepatopathies, difficult. Various hepatoprotective compounds like, L-carnitine, are discussed to ameliorate the induced hepatotoxicity.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods:<\/ns4:bold>\n                    Here we aimed to establish a mouse model to study the effect of asparaginases (L-asparaginase and Oncaspar) and L-carnitine on Western-diet-induced hepatosteatosis in mice. Dose-escalation studies were performed to analyze asparaginases induced hepatotoxicity in C57BL\/6 mice with normal or fatty livers. Subsequently, the effect of L-carnitine to improve the induced toxicity was tested.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Results:<\/ns4:bold>\n                    Our results showed mild-to-moderate hepatotoxic effects while the Western-diet induced a higher degree of vacuolization and hepatocyte damage in liver tissue. Testing of L-carnitine in the established models did not show any protective effect on the toxicity or impairment of the efficacy of asparaginases.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Conclusion:<\/ns4:bold>\n                    The here established models were able to demonstrate the asparaginase-induced hepatotoxic effects which were enhanced by the Western-diet. However, to test potential ameliorating drugs, the models might need some improvements.\n                  <\/ns4:p>","DOI":"10.12688\/f1000research.75870.1","type":"journal-article","created":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T08:15:08Z","timestamp":1643703308000},"page":"128","update-policy":"https:\/\/doi.org\/10.12688\/f1000research.crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of asparaginases and L-carnitine on Western-diet-induced hepatosteatosis in mice"],"prefix":"10.12688","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7632-6064","authenticated-orcid":false,"given":"Mona","family":"Ali Mahmoud Assar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"H\u00fcffel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mamdouh","family":"Afify","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3888-0931","authenticated-orcid":false,"given":"Ralf","family":"Weiskirchen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albrecht","family":"Eisert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rene","family":"Tolba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7088-1972","authenticated-orcid":false,"given":"Julia","family":"Steitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2022,2,1]]},"reference":[{"key":"ref1","first-page":"241-254","article-title":"Asparaginase (native ASNase or pegylasted ASNase) in the treatment of acute lymphoblastic leukemia.","volume":"1","author":"V Avramis","year":"2006","journal-title":"Int. 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Avila, Hepatology Program, CIMA, University of Navarra, Pamplona, 07 Feb 2022, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/11-128\/v1#referee-response-121811","order":0,"name":"referee-response-121811","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.79799.r161777, Ruchi Bansal, Translational Liver Research, Department of Medical Cell Biophysics, Technical Medical Center, Faculty of Science and Technology, University of Twente, Enschede, 05 Jul 2023, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/11-128\/v1#referee-response-161777","order":1,"name":"referee-response-161777","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.5256\/f1000research.79799.r182977, Olamide B Adelusi, Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, KS, 24 Jul 2023, version 1, indexed","URL":"https:\/\/f1000research.com\/articles\/11-128\/v1#referee-response-182977","order":2,"name":"referee-response-182977","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported with a stipend to Mona Ali Mahmoud Assar by the Egyptian cultural affairs and missions\u2019 sector and ministry of higher education and by the European Union and NRW government (NRW-EU.Ziel2, (EFRE), 005-0908-0112). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":3,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}