{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:03:09Z","timestamp":1790787789726,"version":"4.1.0"},"update-to":[{"DOI":"10.12688\/gatesopenres.12903.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T00:00:00Z","timestamp":1555372800000}}],"reference-count":49,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T00:00:00Z","timestamp":1555372800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1140230"],"award-info":[{"award-number":["OPP1140230"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o Ezequiel Dias"},{"name":"Brazilian Ministry of Health - DECIT"},{"name":"INCT Dengue"}],"content-domain":{"domain":["gatesopenresearch.org"],"crossmark-restriction":false},"short-container-title":["Gates Open Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Background<\/ns4:bold>\n                    : Yellow fever outbreaks have re-emerged in Brazil during 2016-18, with mortality rates up to 30%. Although urban transmission has not been reported since 1942, the risk of re-urbanization of yellow fever is significant, as\n                    <ns4:italic>Aedes aegypti<\/ns4:italic>\n                    is present in most tropical and sub-tropical cities in the World and still remains the main vector of urban YFV. Although the YFV vaccine is safe and effective, it does not always reach populations at greatest risk of infection and there is an acknowledged global shortage of vaccine supply. The introgression of\n                    <ns4:italic>Wolbachia<\/ns4:italic>\n                    bacteria into\n                    <ns4:italic>Ae. aegypti<\/ns4:italic>\n                    mosquito populations is being trialed in several countries (\n                    <ns4:ext-link xmlns:ns3=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" ns3:href=\"http:\/\/www.worldmosquito.org\">www.worldmosquito.org<\/ns4:ext-link>\n                    ) as a biocontrol method against dengue, Zika and chikungunya. Here, we studied the ability of\n                    <ns4:italic>Wolbachia<\/ns4:italic>\n                    to reduce the transmission potential of\n                    <ns4:italic>Ae. aegypti<\/ns4:italic>\n                    mosquitoes for\n                    <ns4:italic>Yellow fever virus<\/ns4:italic>\n                    (YFV).\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods:<\/ns4:bold>\n                    Two recently isolated YFV (primate and human) were used to challenge field-derived wild-type and\n                    <ns4:italic>Wolbachia<\/ns4:italic>\n                    -infected (\n                    <ns4:italic>w<\/ns4:italic>\n                    Mel +)\n                    <ns4:italic>Ae. aegypti<\/ns4:italic>\n                    mosquitoes. The YFV infection status was followed for 7, 14 and 21 days post-oral feeding (dpf). The YFV transmission potential of mosquitoes was evaluated via nano-injection of saliva into uninfected mosquitoes or by inoculation in mice.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Results:<\/ns4:bold>\n                    We found that\n                    <ns4:italic>Wolbachia<\/ns4:italic>\n                    was able to significantly reduce the prevalence of mosquitoes with YFV infected heads and thoraces for both viral isolates. Furthermore, analyses of mosquito saliva, through indirect injection into na\u00efve mosquitoes or via interferon-deficient mouse model, indicated\n                    <ns4:italic>Wolbachia<\/ns4:italic>\n                    was associated with profound reduction in the YFV transmission potential of mosquitoes (14dpf).\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Conclusions:<\/ns4:bold>\n                    Our results suggest that\n                    <ns4:italic>Wolbachia<\/ns4:italic>\n                    introgression could be used as a complementary strategy for prevention of urban yellow fever transmission, along with the human vaccination program.\n                  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e","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago Nunes","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1471-0084","authenticated-orcid":false,"given":"Talita \u00c9mile Ribeiro","family":"Adelino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Giovanetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Carlos Junior","family":"Alcantara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franciele Martins","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor Rodrigues de Melo","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro Martins","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3629-8529","authenticated-orcid":false,"given":"Felipe Campos de Melo","family":"Iani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0175-642X","authenticated-orcid":false,"given":"Vivian Vasconcelos","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano 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status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.21956\/gatesopenres.14075.r27067, Andrew van den Hurk, School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, Queensland, Australia, 23 Apr 2019, version 2, indexed","URL":"https:\/\/gatesopenresearch.org\/articles\/3-161\/v2#referee-response-27067","order":0,"name":"referee-response-27067","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/gatesopenres.14075.r27068, Jayme A. Souza-Neto, Bianca C. Carlos, School of Agricultural Sciences,  Department of Bioprocesses and Biotechnology, Multiuser Central Laboratory, S\u00e3o Paulo State University (UNESP), Botucatu, Brazil, 17 Apr 2019, version 2, indexed","URL":"https:\/\/gatesopenresearch.org\/articles\/3-161\/v2#referee-response-27068","order":1,"name":"referee-response-27068","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"Bill Melinda Gates Foundation through Monash University and the Brazilian Ministry of Health (DECIT) [OPP1140230]. This work was partially supported by the National Institute of Science and Technology in Dengue and Host-microorganism Interaction (INCT Dengue), and the Minas Gerais Foundation for Science (FAPEMIG, Brazil). LAM and MMT are fellows from CNPq, Brazil. This work also received support from the Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES) and Funda\u00e7\u00e3o Ezequiel Dias (FUNED). LCJA, FCMI and MG have used sequencing primers and protocols from the ZIBRA2 project funded from CNPq and CAPES (440685\/2016-8 and 88887.130716\/2016-00). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}