{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T19:18:46Z","timestamp":1792178326076,"version":"4.4.0"},"reference-count":27,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T00:00:00Z","timestamp":1761264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the CGIAR initiative on Plant Health, supported by the donors of the CGIAR Trust Fund"}],"content-domain":{"domain":["gatesopenresearch.org"],"crossmark-restriction":false},"short-container-title":["Gates Open Res"],"abstract":"<ns3:p>\n                    The sweetpotato (\n                    <ns3:italic>Ipomoea batatas<\/ns3:italic>\n                    ) is an important food crop in the tropical and subtropical regions of the world, but its yield and quality are heavily affected by viral diseases. Timely and precise detection of virus infections is essential for effective monitoring of seed health and disease management. We evaluated the feasibility of using a compact, ultra-portable LAMP-based diagnostic device\u2014initially designed for human health applications\u2014for detecting key sweetpotato viruses (SPCSV, SPFMV, and SPLCV). Field and greenhouse samples were tested, showing 100% agreement in virus detection with a larger commonly used LAMP device. Sensitivity tests confirmed consistent performance, and the use of portable power banks enabled reliable on-site use. The statistical analysis indicated high accuracy and strong correlation in time-to-positive values between methods (r &gt; 0.89, p &lt; 0.01). Furthermore, cost analysis demonstrated that the pocket LAMP device setup significantly reduced per-test costs\u2014by approximately 40%\u2014while maintaining diagnostics reliability. These findings support the potential of this tool on plant virus detection in locations with limited resources.\n                  <\/ns3:p>","DOI":"10.12688\/gatesopenres.16369.1","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:55:16Z","timestamp":1763686516000},"page":"91","update-policy":"https:\/\/doi.org\/10.12688\/gatesopenres.crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of an ultra-portable pocket-sized device for running Loop mediated isothermal amplification (LAMP) assays for rapid detection of sweetpotato viruses"],"prefix":"10.12688","volume":"9","author":[{"given":"Segundo","family":"Fuentes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwame","family":"Ogero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Perez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan Frederick","family":"Kreuze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2025,10,24]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"454-458","DOI":"10.2144\/000112149","article-title":"Alkaline polyethylene glycol-based method for direct PCR from bacteria, eukaryotic tissue samples, and whole blood.","volume":"40","author":"P Chomczynski","year":"2006","journal-title":"BioTechniques."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"168-185","DOI":"10.1094\/PDIS-07-11-0550","article-title":"Sweetpotato viruses: 15 years of progress on understanding and managing complex diseases.","volume":"96","author":"C Clark","year":"2012","journal-title":"Plant Dis."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"459-471","DOI":"10.1111\/mpp.12200","article-title":"Synergistic interactions of begomoviruses with Sweet potato chlorotic stunt virus (genus Crinivirus) in sweet potato (Ipomoea batatas L.).","volume":"16","author":"W Cuellar","year":"2015","journal-title":"Mol. Plant Pathol."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"284","DOI":"10.3390\/agriculture14020284","article-title":"Dissecting diagnostic and management strategies for plant viral diseases: What next?","volume":"14","author":"B Devi","year":"2024","journal-title":"Agriculture."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"1-8","DOI":"10.1016\/j.ijid.2022.07.042","article-title":"An isothermal lab-on-phone test for easy molecular diagnosis of SARS-CoV-2 near patients and in less than 1 hour.","volume":"123","author":"G Doria","year":"2022","journal-title":"Int. J. Infect. Dis."},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.6084\/m9.figshare.29109401","author":"S Fuentes","year":"2025","journal-title":"figshare."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"297-302","DOI":"10.1094\/PDIS.2003.87.3.297","article-title":"Sweetpotato virus disease (SPVD): Distribution, incidence, and effect on sweetpotato yield in Peru.","volume":"87","author":"D Guti\u00e9rrez","year":"2003","journal-title":"Plant Dis."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"105220","DOI":"10.1016\/J.JFCA.2023.105220","article-title":"Towards achieving online prediction of starch in postharvest sweet potato (Ipomoea batatas L.) by NIR combined with linear algorithm.","volume":"118","author":"H He","year":"2023","journal-title":"J. Food Compos. 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Plant Sci."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"63-76","DOI":"10.1007\/978-3-030-57899-2_5","volume":"10","author":"J Kreuze","year":"2021","journal-title":"Plant diseases and food security in the 21st century."},{"key":"ref10","doi-asserted-by":"publisher","first-page":"199276","DOI":"10.1016\/j.virusres.2023.199276","article-title":"High-throughput characterization and phenotyping of resistance and tolerance to virus infection in sweetpotato.","volume":"339","author":"J Kreuze","year":"2024","journal-title":"Virus Res."},{"key":"ref11","doi-asserted-by":"publisher","first-page":"97-125","DOI":"10.1007\/978-0-387-92765-7_3","volume":"7","author":"V Lebot","year":"2010","journal-title":"Root and tuber crops."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"1169-1178","DOI":"10.1099\/jmm.0.001238","article-title":"Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNA.","volume":"69","author":"J Lee","year":"2020","journal-title":"J. Med. Microbiol."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"33-45","DOI":"10.1016\/bs.aivir.2014.10.005","article-title":"Control of sweet potato virus diseases.","volume":"91","author":"G Loebenstein","year":"2015","journal-title":"Adv. Virus Res."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1186\/s13007-024-01175-6","article-title":"Assessing the effect of sample storage time on viral detection using a rapid and cost-effective CTAB-based extraction method.","volume":"20","author":"D Mark","year":"2024","journal-title":"Plant Methods."},{"key":"ref16","doi-asserted-by":"publisher","first-page":"961-968","DOI":"10.1007\/s42161-021-00830-4","article-title":"Incidence and distribution of sweetpotato viruses and their implication on sweetpotato seed system in Malawi.","volume":"103","author":"W Mbewe","year":"2021","journal-title":"J. Plant Pathol."},{"key":"ref27","doi-asserted-by":"publisher","first-page":"e63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA.","volume":"28","author":"T Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref17","doi-asserted-by":"publisher","first-page":"342-365","DOI":"10.1525\/aa.1972.74.3.02a00070","article-title":"The sweet potato: Its origin and dispersal.","volume":"74","author":"P O\u2019Brien","year":"1972","journal-title":"Am. Anthropol."},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-35512-8_29-1","author":"K Ogero","year":"2023","journal-title":"Handbook of vegetable and herb diseases."},{"key":"ref19","article-title":"mcr: Method comparison regression (Version 1.3.2) [R package].","author":"S Potapov","year":"2023"},{"key":"ref20","doi-asserted-by":"publisher","first-page":"e00561","DOI":"10.1016\/j.heliyon.2018.e00561","article-title":"Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood.","volume":"4","author":"N Quoc","year":"2018","journal-title":"Heliyon."},{"key":"ref21","doi-asserted-by":"publisher","first-page":"11931","DOI":"10.1038\/s41598-023-38860-w","article-title":"LAMP (loop-mediated isothermal amplification) assay for rapid identification of Varroa mites.","volume":"13","author":"L Rako","year":"2023","journal-title":"Sci. Rep."},{"key":"ref22","doi-asserted-by":"publisher","first-page":"1003-1017","DOI":"10.1111\/ppa.13190","article-title":"Characterization of virus species associated with sweetpotato virus diseases in Burkina Faso.","volume":"69","author":"E Tibiri","year":"2020","journal-title":"Plant Pathol."},{"key":"ref23","doi-asserted-by":"publisher","first-page":"669-676","DOI":"10.1094\/PDIS-91-6-0669","article-title":"Synergistic interaction of Sweet potato chlorotic stunt virus (Crinivirus) with Carla-, Cucumo-, Ipomo-, and Potyviruses infecting sweet potato.","volume":"91","author":"M Untiveros","year":"2007","journal-title":"Plant Dis."},{"key":"ref25","doi-asserted-by":"publisher","first-page":"1477-1486","DOI":"10.1094\/PDIS-06-19-1196-RE","article-title":"Storage root yield of sweetpotato as influenced by Sweet potato leaf curl virus and its interaction with Sweet potato feathery mottle virus and Sweet potato chlorotic stunt virus in Kenya.","volume":"104","author":"B Wanjala","year":"2020","journal-title":"Plant Dis."},{"key":"ref24","doi-asserted-by":"publisher","first-page":"1183-1192","DOI":"10.1002\/csc2.21203","article-title":"Loop-mediated isothermal amplification assay: A novel disease diagnostics tool in sweetpotato seed quality assurance.","volume":"64","author":"B Wanjala","year":"2024","journal-title":"Crop Sci."},{"key":"ref26","doi-asserted-by":"publisher","first-page":"191-204","DOI":"10.1007\/s12230-025-09986-6","article-title":"Development of an innovative loop-mediated isothermal amplification (LAMP) assay for the rapid on-site detection of Phytophthora infestans.","volume":"102","author":"P Wharton","year":"2025","journal-title":"Am. 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Potato Res."}],"container-title":["Gates Open Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/gatesopenresearch.org\/articles\/9-91\/v1\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/gatesopenresearch.org\/articles\/9-91\/v1\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/gatesopenresearch.org\/articles\/9-91\/v1\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:55:22Z","timestamp":1763686522000},"score":1,"resource":{"primary":{"URL":"https:\/\/gatesopenresearch.org\/articles\/9-91\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,24]]},"references-count":27,"URL":"https:\/\/doi.org\/10.12688\/gatesopenres.16369.1","relation":{"has-preprint":[{"id-type":"doi","id":"10.12688\/verixiv.1161.1","asserted-by":"subject"},{"id-type":"doi","id":"10.12688\/verixiv.1161.1","asserted-by":"object"}]},"ISSN":["2572-4754"],"issn-type":[{"value":"2572-4754","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,24]]},"assertion":[{"value":"Awaiting Peer Review","URL":"https:\/\/gatesopenresearch.org\/articles\/9-91\/v1#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"This research was funded by the Gates Foundation (BMGF), through its investment (INV-040803) awarded to the International Institute of Tropical Agriculture (PROSSIVA) and the CGIAR initiative on Plant Health, supported by the donors of the CGIAR Trust Fund (http:\/\/www.cgiar.org\/funders\/).","order":0,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}