{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T22:55:04Z","timestamp":1790463304570,"version":"4.1.0"},"update-to":[{"DOI":"10.12688\/wellcomeopenres.11558.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2018,5,11]],"date-time":"2018-05-11T00:00:00Z","timestamp":1525996800000}}],"reference-count":27,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2018,5,11]],"date-time":"2018-05-11T00:00:00Z","timestamp":1525996800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wellcome Trust","award":["101104"],"award-info":[{"award-number":["101104"]}]},{"name":"Wellcome Trust","award":["204904"],"award-info":[{"award-number":["204904"]}]},{"name":"Wellcome Trust","award":["093724"],"award-info":[{"award-number":["093724"]}]},{"name":"Wellcome Trust","award":["106680"],"award-info":[{"award-number":["106680"]}]}],"content-domain":{"domain":["wellcomeopenresearch.org"],"crossmark-restriction":false},"short-container-title":["Wellcome Open Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Background:<\/ns4:bold>\n                    Since 1962, enterovirus D68 (EV-D68) has been implicated in multiple outbreaks and sporadic cases of respiratory infection worldwide, especially in the USA and Europe with an increasing frequency between 2010 and 2014. We describe the detection, associated clinical features and molecular characterization of EV-D68 in central and southern Viet Nam between 2009 and 2015.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods:<\/ns4:bold>\n                    Enterovirus\/rhinovirus PCR positive respiratory or CSF samples taken from children and adults with respiratory\/central nervous system infections in Viet Nam were tested by an EV-D68 specific PCR. The included samples were derived from 3 different observational studies conducted at referral hospitals across central and southern Viet Nam 2009 \u00a02015. Whole-genome sequencing was carried out using a MiSeq based approach. Phylogenetic reconstruction and estimation of evolutionary rate and recombination were carried out in BEAST and Recombination Detection Program, respectively.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Results:<\/ns4:bold>\n                    EV-D68 was detected in 21\/625 (3.4%) enterovirus\/rhinovirus PCR positive respiratory samples but in none of the 15 CSF. All the EV-D68 patients were young children (age range: 11.8 \u2013 24.5 months) and had moderate respiratory infections. Phylogenetic analysis suggested that the Vietnamese sequences clustered with those from Asian countries, of which 9 fell in the B1 clade, and the remaining sequence was identified within the A2 clade. One intra sub-clade recombination event was detected, representing the second reported recombination within EV-D68. The evolutionary rate of EV-D68 was estimated to be 5.12E\n                    <ns4:sup>-3<\/ns4:sup>\n                    substitutions\/site\/year. Phylogenetic analysis indicated that the virus was imported into Viet Nam in 2008.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Conclusions:<\/ns4:bold>\n                    We have demonstrated for the first time EV-D68 has been circulating at low levels in Viet Nam since 2008, associated with moderate acute respiratory infection in children. EV-D68 in Viet Nam is most closely related to Asian viruses, and clusters separately from recent US and European viruses that were suggested to be associated with acute flaccid paralysis.\n                  <\/ns4:p>","DOI":"10.12688\/wellcomeopenres.11558.2","type":"journal-article","created":{"date-parts":[[2018,5,11]],"date-time":"2018-05-11T05:00:08Z","timestamp":1526014808000},"page":"41","update-policy":"https:\/\/doi.org\/10.12688\/wellcomeopenres.crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Enterovirus D68 in Viet Nam (2009-2015)"],"prefix":"10.12688","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9833-2921","authenticated-orcid":false,"given":"Nguyen Thi Han","family":"Ny","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8814-4436","authenticated-orcid":false,"given":"Nguyen To","family":"Anh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1085-4491","authenticated-orcid":false,"given":"Vu Thi Ty","family":"Hang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lam Anh","family":"Nguyet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8096-7750","authenticated-orcid":false,"given":"Tran Tan","family":"Thanh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do Quang","family":"Ha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ngo Ngoc Quang","family":"Minh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Do Lien Anh","family":"Ha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"McBride","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3299-7584","authenticated-orcid":false,"given":"Ha Manh","family":"Tuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Baker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pham Thi Thanh","family":"Tam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tran My","family":"Phuc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2744-781X","authenticated-orcid":false,"given":"Dang Thao","family":"Huong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tran Quoc","family":"Loi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Tran Anh","family":"Vu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Van","family":"Hung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tran Thi Thuy","family":"Minh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Van","family":"Xang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho Dang Trung","family":"Nghia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Van Vinh","family":"Chau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2858-2087","authenticated-orcid":false,"given":"Guy","family":"Thwaites","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9807-1821","authenticated-orcid":false,"given":"H. Rogier","family":"van Doorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Anscombe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1791-3901","authenticated-orcid":false,"given":"Tan","family":"Le Van","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"VIZIONS Consortium","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2018,5,11]]},"reference":[{"key":"ref-1","doi-asserted-by":"publisher","first-page":"297-310","DOI":"10.1093\/oxfordjournals.aje.a120693","article-title":"A probable new human picornavirus associated with respiratory diseases.","volume":"85","author":"J Schieble","year":"1967","journal-title":"Am J Epidemiol."},{"key":"ref-2","doi-asserted-by":"publisher","first-page":"4218-23","DOI":"10.1128\/JCM.40.11.4218-4223.2002","article-title":"Human rhinovirus 87 and enterovirus 68 represent a unique serotype with rhinovirus and enterovirus features.","volume":"40","author":"S Blomqvist","year":"2002","journal-title":"J Clin Microbiol."},{"key":"ref-3","doi-asserted-by":"publisher","first-page":"2577-84","DOI":"10.1099\/vir.0.79925-0","article-title":"Enterovirus 68 is associated with respiratory illness and shares biological features with both the enteroviruses and the rhinoviruses.","volume":"85","author":"M Oberste","year":"2004","journal-title":"J Gen Virol."},{"key":"ref-4","doi-asserted-by":"publisher","first-page":"671-82","DOI":"10.1016\/S1473-3099(15)70093-9","article-title":"A novel outbreak enterovirus D68 strain associated with acute flaccid myelitis cases in the USA (2012\u201314): a retrospective cohort study.","volume":"15","author":"A Greninger","year":"2015","journal-title":"Lancet Infect Dis."},{"key":"ref-5","doi-asserted-by":"publisher","first-page":"737-745","DOI":"10.1093\/cid\/ciw372","article-title":"Acute Flaccid Myelitis in the United States, August-December 2014: Results of Nationwide Surveillance.","volume":"63","author":"J Sejvar","year":"2016","journal-title":"Clin Infect Dis."},{"key":"ref-6","doi-asserted-by":"publisher","first-page":"e61-2","DOI":"10.1016\/j.cmi.2015.05.008","article-title":"Enterovirus D68 nosocomial outbreak in elderly people, France, 2014.","volume":"21","author":"A Bal","year":"2015","journal-title":"Clin Microbiol Infect."},{"key":"ref-7","doi-asserted-by":"publisher","first-page":"1015-8","DOI":"10.1128\/JCM.03362-14","article-title":"Molecular epidemiology of enterovirus D68 from 2013 to 2014 in Philippines.","volume":"53","author":"Y Furuse","year":"2015","journal-title":"J Clin Microbiol."},{"key":"ref-8","doi-asserted-by":"publisher","first-page":"117-21","DOI":"10.1016\/j.jcv.2015.06.079","article-title":"Enterovirus D68 disease and molecular epidemiology in Australia.","volume":"69","author":"A Levy","year":"2015","journal-title":"J Clin Virol."},{"key":"ref-9","doi-asserted-by":"publisher","first-page":"384","DOI":"10.3201\/eid2102.141742","article-title":"Genome sequence of enterovirus D68 and clinical disease, Thailand.","volume":"21","author":"S Vongpunsawad","year":"2015","journal-title":"Emerg Infect Dis."},{"key":"ref-10","doi-asserted-by":"publisher","first-page":"916-8","DOI":"10.3201\/eid2105.150036","article-title":"Enterovirus D68-associated severe pneumonia, China, 2014.","volume":"21","author":"T Zhang","year":"2015","journal-title":"Emerg Infect Dis."},{"key":"ref-11","doi-asserted-by":"publisher","first-page":"e0160606","DOI":"10.1371\/journal.pone.0160606","article-title":"Respiratory Syncytial Virus and Other Viral Infections among Children under Two Years Old in Southern Vietnam 2009\u20132010: Clinical Characteristics and Disease Severity","volume":"11","author":"L Do","year":"2016","journal-title":"PLoS One."},{"key":"ref-12","article-title":"Outpatient Antibiotic Use in Acute Respiratory Infections","author":"N Ngoc","year":"2014"},{"key":"ref-13","doi-asserted-by":"publisher","first-page":"726-35","DOI":"10.1007\/s10393-015-1061-0","article-title":"The Vietnam Initiative on Zoonotic Infections (VIZIONS): A Strategic Approach to Studying Emerging Zoonotic Infectious Diseases.","volume":"12","author":"M Rabaa","year":"2015","journal-title":"EcoHealth."},{"key":"ref-14","doi-asserted-by":"publisher","first-page":"179-85","DOI":"10.1016\/j.jcv.2011.04.010","article-title":"Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts.","volume":"51","author":"R Jansen","year":"2011","journal-title":"J Clin Virol."},{"key":"ref-15","doi-asserted-by":"publisher","first-page":"1915-20","DOI":"10.1128\/JCM.00358-15","article-title":"Evaluation of a Real-Time Reverse Transcription-PCR Assay for Detection of Enterovirus D68 in Clinical Samples from an Outbreak in New York State in 2014.","volume":"53","author":"J Zhuge","year":"2015","journal-title":"J Clin Microbiol."},{"key":"ref-16","doi-asserted-by":"publisher","first-page":"e16118","DOI":"10.1371\/journal.pone.0016118","article-title":"A sensitive assay for virus discovery in respiratory clinical samples.","volume":"6","author":"M de Vries","year":"2011","journal-title":"PLoS One."},{"key":"ref-17","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1186\/s12985-016-0580-9","article-title":"Development and evaluation of a non-ribosomal random PCR and next-generation sequencing based assay for detection and sequencing of hand, foot and mouth disease pathogens.","volume":"13","author":"A Nguyen","year":"2016","journal-title":"Virol J."},{"key":"ref-18","doi-asserted-by":"publisher","first-page":"1647-9","DOI":"10.1093\/bioinformatics\/bts199","article-title":"Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data.","volume":"28","author":"M Kearse","year":"2012","journal-title":"Bioinformatics."},{"key":"ref-19","doi-asserted-by":"publisher","first-page":"vev003","DOI":"10.1093\/ve\/vev003","article-title":"RDP4: Detection and analysis of recombination patterns in virus genomes.","volume":"1","author":"D Martin","year":"2015","journal-title":"Virus Evol."},{"key":"ref-20","doi-asserted-by":"publisher","first-page":"1969-73","DOI":"10.1093\/molbev\/mss075","article-title":"Bayesian phylogenetics with BEAUti and the BEAST 1.7.","volume":"29","author":"A Drummond","year":"2012","journal-title":"Mol Biol Evol."},{"key":"ref-21","doi-asserted-by":"publisher","first-page":"126-32","DOI":"10.1016\/j.jcv.2016.07.014","article-title":"Epidemiology and etiology of influenza-like-illness in households in Vietnam; it\u2019s not all about the kids!","volume":"82","author":"D Nguyen","year":"2016","journal-title":"J Clin Virol."},{"key":"ref-22","doi-asserted-by":"publisher","DOI":"10.1038\/srep27800","article-title":"Genetic divergence of enterovirus D68 in China and the United States.","volume":"6","author":"Z Xiang","year":"2016","journal-title":"Sci Rep."},{"key":"ref-23","doi-asserted-by":"publisher","first-page":"e35190","DOI":"10.1371\/journal.pone.0035190","article-title":"Molecular epidemiology and evolution of human enterovirus serotype 68 in Thailand, 2006\u20132011.","volume":"7","author":"P Linsuwanon","year":"2012","journal-title":"PLoS One."},{"key":"ref-24","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1186\/s12985-014-0224-x","article-title":"Multiplex PCR analysis of clusters of unexplained viral respiratory tract infection in Cambodia.","volume":"11","author":"N Ly","year":"2014","journal-title":"Virol J."},{"key":"ref-25","doi-asserted-by":"publisher","first-page":"140-3","DOI":"10.3201\/eid2301.161313","article-title":"Upsurge of Enterovirus D68, the Netherlands, 2016.","volume":"23","author":"M Knoester","year":"2017","journal-title":"Emerg Infect Dis."},{"key":"ref-26","doi-asserted-by":"publisher","first-page":"1997-2007","DOI":"10.1128\/JVI.02418-15","article-title":"Molecular Evolution and Intraclade Recombination of Enterovirus D68 during the 2014 Outbreak in the United States.","volume":"90","author":"Y Tan","year":"2015","journal-title":"J Virol."},{"key":"ref-27","doi-asserted-by":"publisher","first-page":"e0144208","DOI":"10.1371\/journal.pone.0144208","article-title":"Analysis of Enterovirus 68 Strains from the 2014 North American Outbreak Reveals a New Clade, Indicating Viral Evolution.","volume":"10","author":"J Du","year":"2015","journal-title":"PLoS One."}],"container-title":["Wellcome Open Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/wellcomeopenresearch.org\/articles\/2-41\/v2\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/wellcomeopenresearch.org\/articles\/2-41\/v2\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/wellcomeopenresearch.org\/articles\/2-41\/v2\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T08:59:48Z","timestamp":1589878788000},"score":1,"resource":{"primary":{"URL":"https:\/\/wellcomeopenresearch.org\/articles\/2-41\/v2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,11]]},"references-count":27,"URL":"https:\/\/doi.org\/10.12688\/wellcomeopenres.11558.2","relation":{"has-review":[{"id-type":"doi","id":"10.21956\/wellcomeopenres.15891.r33134","asserted-by":"subject"},{"id-type":"doi","id":"10.21956\/wellcomeopenres.15891.r33134","asserted-by":"object"}]},"ISSN":["2398-502X"],"issn-type":[{"value":"2398-502X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,11]]},"assertion":[{"value":"Indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/2-41\/v2#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.21956\/wellcomeopenres.15891.r33134, Weihua Huang, Department of Pathology, New York Medical College, Vahalla, NY, USA, 11 May 2018, version 2, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/2-41\/v2#referee-response-33134","order":0,"name":"referee-response-33134","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/wellcomeopenres.12486.r24363, Kristine M. Wylie, Department of Pediatrics and The Genome Institute, Washington University School of Medicine, St. Louis, MO, USA, 31 Jul 2017, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/2-41\/v1#referee-response-24363","order":1,"name":"referee-response-24363","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Nguyen Thi Han Ny<\/b>; \n<i>Posted: 03 May 2018<\/i>; This is a nicely written report describing Enterovirus D68 prevalence and genome sequences in Vietnam. I have only minor comments.  The authors cite a real-time PCR assay that is specific for Enterovirus D68. I believe this paper cites the assay developed by the CDC. If so, the primary CDC reference should be cited. Does this assay detect viruses from all of the clades? If not or if it is unclear, this should be described as a limitation of the study.  \n<b>Response<\/b>: We would like to thank you for your comments and suggestions.&nbsp; In regards to the real-time method for EV-D68 detection we have added a reference to the original paper describing and evaluating the CDC method. As originally designed the assay should be able to detect viruses from Asia, the US and Europe. However the diagnostic utility of the assay has only been conducted in the US, and therefore some SE-Asian clades may have been missed. We added some sentences in the discussion to elaborate this limitation. &nbsp;  Some additional details regarding the PCR assay and sequencing assays would be useful for reproducibility. Was there absolutely no deviation from the cited papers? What enzymes were used? RT-PCR instrument? How much material was used as input for the sequencing assays? What were the specific library construction methods?  \n<b>Response<\/b>: Additional information about the PCR assay and whole genome sequencing approach has been added to the methods section. &nbsp;  In Figures 2 and 3, it is difficult to distinguish the dark and light blue colors. A color with more contrast should be selected for the Vietnam samples.  \n<b>Response<\/b>: Colours on the phylogenetic trees have been changed to show the Vietnamese isolates clearer. &nbsp;  There seems to be a formatting issue. Sporadically lowercase &quot;f&quot; is replaced with an odd symbol in my copy of the paper. There are several examples in the second paragraph of the introduction, but this occurs elsewhere in the paper, too.  \n<b>Response<\/b>: Please forgive our ignorance. But we have thoroughly checked the document on more than one computer and could not found any formatting issues.","URL":"https:\/\/wellcomeopenresearch.org\/articles\/2-41\/v2#referee-comment-3607","order":2,"name":"referee-comment-3607","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/wellcomeopenres.12486.r24362, Hitoshi Oshitani, Department of Virology, Tohoku University Graduate School of Medicine, Sendai, Japan, 07 Aug 2017, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/2-41\/v1#referee-response-24362","order":3,"name":"referee-response-24362","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Nguyen Thi Han Ny<\/b>; \n<i>Posted: 03 May 2018<\/i>; The manuscript is well written and technically sound. But I have a few minor comments which might be helpful to improve the manuscript.&nbsp;  In the introduction, the authors mentioned that EV-D68 is a genotype. It is true that typing of enteroviruses including EV-D68 is currently done mainly by genetic analysis. But many of enteroviruses including EV-D68 were originally classified as serotypes by using serological tests. I don\u2019t think a genotype is appropriate.  \n<b>Response<\/b>: Thank you for your comment. We have updated the paper to use serotype to describe EV-D68 throughout.&nbsp;  In line 7 of introduction, I am not sure what the authors would like to explain about 5\u2019UTR.  \n<b>Response: <\/b>A sentence describing the 5\u2019 UTR has been updated to make our point clearer.  They mentioned that all EV-D68 cases had \u2018moderate respiratory illness\u2019. But the definition of moderate respiratory illness is unclear.  \n<b>Response<\/b>: We have revised this phrase to avoid confusion. &nbsp;Accordingly the sentence now reads \u201cAll of 21 EV-D68 positive patients were aged less than 2 years, presented with a respiratory illness of short duration and none needed admission to intensive care units.\u201d&nbsp;  They collected samples from 2009 and 2015. But it is not mentioned in which year EV-D68 were detected.  \n<b>Response: <\/b>We have added details of the earliest detection of EV-D68 in our study.&nbsp;","URL":"https:\/\/wellcomeopenresearch.org\/articles\/2-41\/v2#referee-comment-3606","order":4,"name":"referee-comment-3606","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported by the Wellcome Trust, UK [101104\/Z\/13\/Z, 106680\/B\/14\/Z, 093724\/Z\/10\/Z and 204904\/Z\/16\/Z]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":5,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}