{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:19:48Z","timestamp":1790835588229,"version":"4.1.0"},"reference-count":18,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T00:00:00Z","timestamp":1515456000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["107475"],"award-info":[{"award-number":["107475"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["wellcomeopenresearch.org"],"crossmark-restriction":false},"short-container-title":["Wellcome Open Res"],"abstract":"<ns4:p>\n                    Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different\n                    <ns4:italic>Schistosoma<\/ns4:italic>\n                    species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of\n                    <ns4:italic>ex vivo<\/ns4:italic>\n                    modifications, such as those used in functional genomics studies.\n                  <\/ns4:p>","DOI":"10.12688\/wellcomeopenres.13488.1","type":"journal-article","created":{"date-parts":[[2018,1,9]],"date-time":"2018-01-09T12:25:45Z","timestamp":1515500745000},"page":"3","update-policy":"https:\/\/doi.org\/10.12688\/wellcomeopenres.crossmark-policy","source":"Crossref","is-referenced-by-count":20,"title":["Transplantation of schistosome sporocysts between host snails: A video guide"],"prefix":"10.12688","volume":"3","author":[{"given":"Gabriel","family":"Mouahid","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Rognon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1147-0043","authenticated-orcid":false,"given":"Ronaldo","family":"de Carvalho Augusto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Driguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy","family":"Geyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2079-0973","authenticated-orcid":false,"given":"Shannon","family":"Karinshak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7929-902X","authenticated-orcid":false,"given":"Nelia","family":"Luviano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Mann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Quack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate","family":"Rawlinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Wendt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2161-412X","authenticated-orcid":false,"given":"Christoph","family":"Grunau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Mon\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2018,1,9]]},"reference":[{"key":"ref-1","doi-asserted-by":"publisher","first-page":"785-793","DOI":"10.1590\/S0074-02762011000700002","article-title":"Progress with schistosome transgenesis.","volume":"106","author":"Y Alrefaei","year":"2011","journal-title":"Mem Inst Oswaldo Cruz."},{"key":"ref-2","doi-asserted-by":"publisher","first-page":"473-482","DOI":"10.2307\/3276313","article-title":"Transplantation of larval Schistosoma mansoni from infected to uninfected snails.","volume":"52","author":"E Chernin","year":"1966","journal-title":"J Parasitol."},{"key":"ref-3","first-page":"27-30","article-title":"Mise en \u00e9vidence de l\u2019intervention de sporocystes r\u00e9plicateurs dans le cycle biologique de Schistosoma bovis (Sonsino, 1876) Blanchard, 1895.","volume":"1","author":"C Combes","year":"1983","journal-title":"Bulletin de la Soci\u00e9t\u00e9 Fran\u00e7aise de Parasitologie."},{"key":"ref-4","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2014.19.22.20821","article-title":"Schistosoma haematobium infections acquired in Corsica, France, August 2013.","volume":"19","author":"M Holtfreter","year":"2014","journal-title":"Euro Surveill."},{"key":"ref-5","doi-asserted-by":"publisher","first-page":"816-821","DOI":"10.1126\/science.1225829","article-title":"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.","volume":"337","author":"M Jinek","year":"2012","journal-title":"Science."},{"key":"ref-6","first-page":"155-157","article-title":"Apports des techniques de transplantation \u00e0 la connaissance de la productivit\u00e9 de Schistosoma mansoni chez le mollusque vecteur Biomphalaria glabrata.","volume":"8","author":"J Jourdane","year":"1977","journal-title":"Haliotis."},{"key":"ref-7","doi-asserted-by":"publisher","first-page":"217-223","DOI":"10.1051\/parasite\/1989643217","article-title":"Le clonage des schistosomes: ses applications aux recherches modernes sur la biologie des Schistosomes.","volume":"64","author":"J Jourdane","year":"1989","journal-title":"Ann Parasitol Hum Comp."},{"key":"ref-8","doi-asserted-by":"publisher","first-page":"349-357","DOI":"10.1016\/0014-4894(80)90038-7","article-title":"Schistosoma mansoni: Cloning by microsurgical transplantation of sporocysts.","volume":"50","author":"J Jourdane","year":"1980","journal-title":"Exp Parasitol."},{"key":"ref-9","doi-asserted-by":"publisher","first-page":"459-466","DOI":"10.1051\/parasite\/1984595459","article-title":"Evolution des sporocystes de Schistosoma bovis apr\u00e8s transplantation microchirurgicale chez Bulinus truncatus.","volume":"59","author":"J Jourdane","year":"1984","journal-title":"Ann Parasitol Hum Comp."},{"key":"ref-10","first-page":"505-508","article-title":"D\u00e9veloppement du tr\u00e9matode Schistosoma haematobium apr\u00e8s transplantation microchirurgicale chez le gast\u00e9ropode Planorbarius metidjensis.","author":"N K\u00e9chemir","year":"1982","journal-title":"Comptes Rendus de l\u2019Acad\u00e9mie des Sciences."},{"key":"ref-11","doi-asserted-by":"publisher","first-page":"451-462","DOI":"10.1017\/S0031182009991211","article-title":"Culture for genetic manipulation of developmental stages of Schistosoma mansoni.","volume":"137","author":"V Mann","year":"2010","journal-title":"Parasitology."},{"key":"ref-12","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.1116997","article-title":"Technique de transplantation microchirurgicale de sporocystes de schistosomes. (Version 1).","author":"G Mouahid","year":"2017a","journal-title":"Zenodo."},{"key":"ref-13","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.1117007","article-title":"Technique of microsurgical transplantation of schistosomes sporocysts (Version 1).","author":"G Mouahid","year":"2017b","journal-title":"Zenodo."},{"key":"ref-14","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.1117009","article-title":"T\u00e9cnica de trasplantaci\u00f3n microquir\u00fargica de esporocistos de Schistosoma (Version 1).","author":"G Mouahid","year":"2017c","journal-title":"Zenodo."},{"key":"ref-15","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.1117011","article-title":"T\u00e9cnica microcir\u00fargica de transplante de esporocistos de esquistossomas. (Version 1).","author":"G Mouahid","year":"2017d","journal-title":"Zenodo."},{"key":"ref-16","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.1117017","article-title":"Manufacture of tools for microsurgical transplantation of schistosoma sporocysts (Version 1).","author":"G Mouahid","year":"2017e","journal-title":"Zenodo."},{"key":"ref-17","doi-asserted-by":"publisher","first-page":"547-554","DOI":"10.1080\/00034983.1981.11687479","article-title":"Dynamics of larval populations of Schistosoma mansoni in Biomphalaria glabrata II. Chronobiology of the intramolluscal larval development during the shedding period.","volume":"75","author":"A Th\u00e9ron","year":"1981","journal-title":"Ann Trop Med Parasitol."},{"key":"ref-18","article-title":"Schistosomiasis"}],"container-title":["Wellcome Open Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/wellcomeopenresearch.org\/articles\/3-3\/v1\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/wellcomeopenresearch.org\/articles\/3-3\/v1\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/wellcomeopenresearch.org\/articles\/3-3\/v1\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T14:14:33Z","timestamp":1570716873000},"score":1,"resource":{"primary":{"URL":"https:\/\/wellcomeopenresearch.org\/articles\/3-3\/v1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,9]]},"references-count":18,"URL":"https:\/\/doi.org\/10.12688\/wellcomeopenres.13488.1","relation":{"has-review":[{"id-type":"doi","id":"10.21956\/wellcomeopenres.14646.r29641","asserted-by":"subject"},{"id-type":"doi","id":"10.21956\/wellcomeopenres.14646.r29642","asserted-by":"subject"},{"id-type":"doi","id":"10.21956\/wellcomeopenres.14646.r29642","asserted-by":"object"},{"id-type":"doi","id":"10.21956\/wellcomeopenres.14646.r29641","asserted-by":"object"}]},"ISSN":["2398-502X"],"issn-type":[{"value":"2398-502X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,9]]},"assertion":[{"value":"Indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/3-3\/v1#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.21956\/wellcomeopenres.14646.r29642, Patrick C. Hanington, School of Public Health, University of Alberta, Edmonton, AB, Canada, 24 Jan 2018, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/3-3\/v1#referee-response-29642","order":0,"name":"referee-response-29642","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/wellcomeopenres.14646.r29641, Christopher J. Bayne, Department of Integrative Biology, College of Science, Oregon State University, Corvallis, OR, USA, 07 Feb 2018, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/3-3\/v1#referee-response-29641","order":1,"name":"referee-response-29641","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported by the Wellcome Trust [107475], the University of Perpignan Via Domitia, the University of Montpellier and the CNRS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}