{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T11:57:03Z","timestamp":1790164623944,"version":"4.1.0"},"reference-count":62,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T00:00:00Z","timestamp":1570579200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["101842"],"award-info":[{"award-number":["101842"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["050662.D10"],"award-info":[{"award-number":["050662.D10"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["090944"],"award-info":[{"award-number":["090944"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["097045"],"award-info":[{"award-number":["097045"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["wellcomeopenresearch.org"],"crossmark-restriction":false},"short-container-title":["Wellcome Open Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Background<\/ns4:bold>\n                    : Cellular proteins vary significantly in both abundance and turnover rates. These parameters depend upon their rates of synthesis and degradation and it is useful to have access to data on protein turnover rates when, for example, designing genetic knock-down experiments or assessing the potential usefulness of covalent enzyme inhibitors. Little is known about the nature and regulation of protein turnover in\n                    <ns4:italic>Trypanosoma brucei<\/ns4:italic>\n                    , the etiological agent of human and animal African trypanosomiasis.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods<\/ns4:bold>\n                    : To establish baseline data on\n                    <ns4:italic>T.<\/ns4:italic>\n                    <ns4:italic>brucei<\/ns4:italic>\n                    proteome turnover, a Stable Isotope Labelling with Amino acids in Cell culture (SILAC)-based mass spectrometry analysis was performed to reveal the synthesis and degradation profiles for thousands of proteins in the bloodstream and procyclic forms of this parasite.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Results<\/ns4:bold>\n                    : This analysis revealed a slower average turnover rate of the procyclic form proteome relative to the bloodstream proteome. As expected, many of the proteins with the fastest turnover rates have functions in the cell cycle and in the regulation of cytokinesis in both bloodstream and procyclic forms. Moreover, the cellular localization of\n                    <ns4:italic>T. brucei<\/ns4:italic>\n                    proteins correlates with their turnover, with mitochondrial and glycosomal proteins exhibiting slower than average turnover rates.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Conclusions<\/ns4:bold>\n                    : The intention of this study is to provide the trypanosome research community with a resource for protein turnover data for any protein or group of proteins. To this end, bioinformatic analyses of these data are made available via an open-access web resource with data visualization functions.\n                  <\/ns4:p>","DOI":"10.12688\/wellcomeopenres.15421.1","type":"journal-article","created":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T11:20:05Z","timestamp":1570620005000},"page":"152","update-policy":"https:\/\/doi.org\/10.12688\/wellcomeopenres.crossmark-policy","source":"Crossref","is-referenced-by-count":36,"title":["Proteome turnover in the bloodstream and procyclic forms of Trypanosoma brucei measured by quantitative proteomics"],"prefix":"10.12688","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0051-017X","authenticated-orcid":false,"given":"Michele","family":"Tinti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Lucia S.","family":"G\u00fcther","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0951-4588","authenticated-orcid":false,"given":"Thomas W. 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Matthews, Institute for Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK, 15 Oct 2019, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/4-152\/v1#referee-response-36719","order":0,"name":"referee-response-36719","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/wellcomeopenres.16864.r36844, Christine Clayton, Department of Microbiology, University of Heidelberg, Heidelberg, D-69120, Germany, 23 Oct 2019, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/4-152\/v1#referee-response-36844","order":1,"name":"referee-response-36844","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/wellcomeopenres.16864.r36717, Julius Lukes, Institute of Parasitology, Biology Centre, Czech Academy of Sciences and Faculty of Science, \u010cesk\u00e9 Bud\u011bjovice, Czech Republic, 28 Oct 2019, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/4-152\/v1#referee-response-36717","order":2,"name":"referee-response-36717","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Michele Tinti<\/b>; \n<i>Posted: 11 Nov 2019<\/i>; The authors would like to thank the reviewers for their thoughtful comments and efforts towards improving our manuscript. We are now working to address the raised points.","URL":"https:\/\/wellcomeopenresearch.org\/articles\/4-152\/v1#article-response-4000","order":3,"name":"article-response-4000","label":"Article Comment","group":{"name":"article-comments","label":"Article Comments"}},{"value":"The contributions of M.T, M.L.S.G and M.A.J.F. were supported by a Wellcome Trust Investigator Award to M.A.J.F. (101842). The contributions of T.W.M.C. were supported by a Wellcome Trust PhD studentship (050662.D10). The contributions of A.I.L. were supported by a Wellcome Trust grant (090944). The University of Dundee Proteomics Facility was supported by Wellcome Trust grant (097045). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":4,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}