{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:11:07Z","timestamp":1772169067051,"version":"3.50.1"},"update-to":[{"DOI":"10.12688\/wellcomeopenres.15852.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T00:00:00Z","timestamp":1605830400000}}],"reference-count":38,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T00:00:00Z","timestamp":1605830400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["404044656"],"award-info":[{"award-number":["404044656"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["104111"],"award-info":[{"award-number":["104111"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["110166"],"award-info":[{"award-number":["110166"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["wellcomeopenresearch.org"],"crossmark-restriction":false},"short-container-title":["Wellcome Open Res"],"abstract":"<ns3:p>\n                    <ns3:bold>Background:<\/ns3:bold>\n                    <ns3:italic>Plasmodium<\/ns3:italic>\n                    parasites rely on various host factors to grow and replicate within red blood cells (RBC). While many host proteins are known that mediate parasite adhesion and invasion, few examples of host enzymes co-opted by the parasite during intracellular development have been described. Recent studies suggested that the host protein Argonaute 2 (Ago2), which is involved in RNA interference, can translocate into the parasite and affect its development. Here, we investigated this hypothesis.\n                  <\/ns3:p>\n                  <ns3:p>\n                    <ns3:bold>Methods<\/ns3:bold>\n                    : We used several different monoclonal antibodies to test for Ago2 localisation in the human malaria parasite,\n                    <ns3:italic>P. falciparum<\/ns3:italic>\n                    and rodent\n                    <ns3:italic>P. berghei<\/ns3:italic>\n                    parasites. In addition, we biochemically fractionated infected red blood cells to localize Ago2. We also quantified parasite growth and sexual commitment in the presence of the Ago2 inhibitor BCI-137.\n                  <\/ns3:p>\n                  <ns3:p>\n                    <ns3:bold>Results<\/ns3:bold>\n                    : Ago2 localization by fluorescence microscopy produced inconclusive results across the three different antibodies, suggesting cross-reactivity with parasite targets. Biochemical separation of parasite and RBC cytoplasm detected Ago2 only in the RBC cytoplasm and not in the parasite. Inhibition of Ago2 using BCl-137 did not result in altered parasite development.\n                  <\/ns3:p>\n                  <ns3:p>\n                    <ns3:bold>Conclusion<\/ns3:bold>\n                    : Ago2 localization in infected RBCs by microscopy is confounded by non-specific binding of antibodies. Complementary results using biochemical fractionation and Ago2 detection by western blot did not detect the protein in the parasite cytosol, and growth assays using a specific inhibitor demonstrated that its catalytical activity is not required for parasite development. We therefore conclude that previous data localising Ago2 to parasite ring stages are due to antibody cross reactivity, and that Ago2 is not required for intracellular\n                    <ns3:italic>Plasmodium<\/ns3:italic>\n                    development.\n                  <\/ns3:p>","DOI":"10.12688\/wellcomeopenres.15852.2","type":"journal-article","created":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T10:56:45Z","timestamp":1605869805000},"page":"92","update-policy":"https:\/\/doi.org\/10.12688\/wellcomeopenres.crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["No evidence for Ago2 translocation from the host erythrocyte into the Plasmodium parasite"],"prefix":"10.12688","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9812-0128","authenticated-orcid":false,"given":"Franziska","family":"Hentzschel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klara","family":"Obrova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1040-9566","authenticated-orcid":false,"given":"Matthias","family":"Marti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2020,11,20]]},"reference":[{"key":"ref-1","article-title":"World Malaria Report 2019","year":"2019"},{"key":"ref-2","doi-asserted-by":"publisher","first-page":"917-928","DOI":"10.1038\/nm.4381","article-title":"Antimalarial drug resistance: Linking Plasmodium falciparum parasite biology to the clinic.","volume":"23","author":"B 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status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.21956\/wellcomeopenres.18055.r41547, Julian Rayner, Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK, 04 Dec 2020, version 2, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/5-92\/v2#referee-response-41547","order":0,"name":"referee-response-41547","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/wellcomeopenres.18055.r41546, Tim W. Gilberger, Department of Biology, University of Hamburg, Hamburg, Germany, 13 Jan 2021, version 2, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/5-92\/v2#referee-response-41546","order":1,"name":"referee-response-41546","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was funded through Wellcome Trust Investigator award 110166 and Wellcome Trust Center award 104111. FH is funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation), Project number 404044656. MM is funded by a Wolfson Merit Royal Society Award. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}