{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T08:40:14Z","timestamp":1792226414112,"version":"4.4.0"},"reference-count":39,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:00:00Z","timestamp":1632960000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012041","name":"Versus Arthritis","doi-asserted-by":"publisher","award":["20200"],"award-info":[{"award-number":["20200"]}],"id":[{"id":"10.13039\/501100012041","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["200183"],"award-info":[{"award-number":["200183"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["wellcomeopenresearch.org"],"crossmark-restriction":false},"short-container-title":["Wellcome Open Res"],"abstract":"<ns3:p>\n                    <ns3:bold>Background:<\/ns3:bold>\n                    Somatosensation depends on primary sensory neurons of the trigeminal and dorsal root ganglia (DRG). Transcriptional profiling of mouse DRG sensory neurons has defined at least 18 distinct neuronal cell types. Using an advillin promoter, we have generated a transgenic mouse line that only expresses diphtheria toxin A (DTA) in sensory neurons in the presence of Cre recombinase. This has allowed us to ablate specific neuronal subsets within the DRG using a range of established and novel Cre lines that encompass all sets of sensory neurons. \u00a0\u00a0\n                  <\/ns3:p>\n                  <ns3:p>\n                    <ns3:bold>Methods:<\/ns3:bold>\n                    A floxed-tdTomato-stop-DTA bacterial artificial chromosome (BAC) transgenic reporter line (AdvDTA) under the control of the mouse advillin DRG promoter was generated. The line was first validated using a Na\n                    <ns3:sub>v<\/ns3:sub>\n                    1.8\n                    <ns3:sup>Cre<\/ns3:sup>\n                    and then crossed to CGRP\n                    <ns3:sup>CreER<\/ns3:sup>\n                    (Calca), Th\n                    <ns3:sup>CreERT2<\/ns3:sup>\n                    , Tmem45b\n                    <ns3:sup>Cre<\/ns3:sup>\n                    , Tmem233\n                    <ns3:sup>Cre<\/ns3:sup>\n                    , Ntng1\n                    <ns3:sup>Cre<\/ns3:sup>\n                    and TrkB\n                    <ns3:sup>CreER<\/ns3:sup>\n                    (Ntrk2) lines. Pain behavioural assays included Hargreaves\u2019, hot plate, Randall-Selitto, cold plantar, partial sciatic nerve ligation and formalin tests.\n                  <\/ns3:p>\n                  <ns3:p>\n                    <ns3:bold>Results:<\/ns3:bold>\n                    Motor activity, as assessed by the rotarod test, was normal for all lines tested. Noxious mechanosensation was significantly reduced when either Na\n                    <ns3:sub>v<\/ns3:sub>\n                    1.8 positive neurons or Tmem45b positive neurons were ablated whilst acute heat pain was unaffected. In contrast, noxious mechanosensation was normal following ablation of CGRP-positive neurons but acute heat pain thresholds were significantly elevated and a reduction in nocifensive responses was observed in the second phase of the formalin test. Ablation of TrkB-positive neurons led to significant deficits in mechanical hypersensitivity in the partial sciatic nerve ligation neuropathic pain model.\n                  <\/ns3:p>\n                  <ns3:p>\n                    <ns3:bold>Conclusions:<\/ns3:bold>\n                    Ablation of specific DRG neuronal subsets using the AdvDTA line will be a useful resource for further functional characterization of somatosensory processing, neuro-immune interactions and chronic pain disorders.\n                  <\/ns3:p>","DOI":"10.12688\/wellcomeopenres.17090.1","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T15:10:10Z","timestamp":1633014610000},"page":"250","update-policy":"https:\/\/doi.org\/10.12688\/wellcomeopenres.crossmark-policy","source":"Crossref","is-referenced-by-count":22,"title":["Tools for analysis and conditional deletion of subsets of sensory neurons"],"prefix":"10.12688","volume":"6","author":[{"given":"Sonia","family":"Santana-Varela","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yury D.","family":"Bogdanov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel J.","family":"Gossage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei L.","family":"Okorokov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengnan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"de Clauser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Alves-Simoes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane E.","family":"Sexton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Iseppon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana P.","family":"Luiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2587-1198","authenticated-orcid":false,"given":"Jing","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9166-8158","authenticated-orcid":false,"given":"John N.","family":"Wood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9129-8011","authenticated-orcid":false,"given":"James J.","family":"Cox","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2021,9,30]]},"reference":[{"key":"ref-1","first-page":"145-159","article-title":"The classification of nerve fibers.","volume":"41","author":"H Gasser","year":"1941","journal-title":"The Ohio Journal of Science."},{"key":"ref-2","doi-asserted-by":"publisher","first-page":"239-43","DOI":"10.1016\/0361-9230(93)90250-f","article-title":"Primary sensory neurones: neurofilament, neuropeptides, and conduction velocity.","volume":"30","author":"S Lawson","year":"1993","journal-title":"Brain Res Bull."},{"key":"ref-3","doi-asserted-by":"publisher","first-page":"145-53","DOI":"10.1038\/nn.3881","article-title":"Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.","volume":"18","author":"D Usoskin","year":"2015","journal-title":"Nat 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Nassar, Department of Biomedical Science, University of Sheffield, Sheffield, UK, 01 Nov 2021, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/6-250\/v1#referee-response-46341","order":0,"name":"referee-response-46341","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/wellcomeopenres.18877.r47926, Uhtaek Oh, Korea Institute of Science and Technology, Seoul, South Korea, 04 Feb 2022, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/6-250\/v1#referee-response-47926","order":1,"name":"referee-response-47926","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported by the Wellcome Trust [200183] and Versus Arthritis [20200]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":2,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}