{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T23:26:48Z","timestamp":1781306808858,"version":"3.54.1"},"update-to":[{"DOI":"10.12688\/wellcomeopenres.22706.1","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000}},{"DOI":"10.12688\/wellcomeopenres.22706.2","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000}}],"reference-count":19,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T00:00:00Z","timestamp":1732752000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["220211"],"award-info":[{"award-number":["220211"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["wellcomeopenresearch.org"],"crossmark-restriction":false},"short-container-title":["Wellcome Open Res"],"abstract":"<ns3:p>\n                    Background\n                    <ns3:italic>Burkholderia thailandensis<\/ns3:italic>\n                    is an environmental bacteria closely related to\n                    <ns3:italic>Burkholderia pseudomallei<\/ns3:italic>\n                    that rarely causes infection in humans. Some environmental isolates have shown to express a capsular polysaccharide known as\n                    <ns3:italic>B. thailandensis<\/ns3:italic>\n                    capsular variant (BTCV), but human infection has not previously been reported. Although\n                    <ns3:italic>B. thailandenisis<\/ns3:italic>\n                    has been identified in environmental samples in Laos before, there have not been any human cases reported. Case A 44-year-old man presented to a district hospital in Laos with a short history of fever and pain in his left foot. Physical examination identified a deep soft-tissue abscess in his left foot and an elevated white blood count. A deep pus sample was taken and melioidosis was suspected from preliminary laboratory tests. The patient was initially started on cloxacillin, ceftriaxone and metronidazole, and was then changed to ceftazidime treatment following local melioidosis treatment guidelines. Laboratory methods A deep pus sample was sent to Mahosot Hospital microbiology laboratory where a mixed infection was identified including\n                    <ns3:italic>Burkholderia<\/ns3:italic>\n                    sp. Conventional identification tests and API 20NE were inconclusive, and the\n                    <ns3:italic>B. pseudomallei<\/ns3:italic>\n                    -specific latex agglutination was positive. The isolate then underwent a\n                    <ns3:italic>Burkholderia<\/ns3:italic>\n                    species specific PCR which identified the isolate as\n                    <ns3:italic>B. thailandensis.<\/ns3:italic>\n                    The isolate was sent for sequencing on the Illumina NovaSeq 6000 system and multi-locus sequence typing analysis identified the isolate had the same sequence type (ST696) as\n                    <ns3:italic>B. thailandensis<\/ns3:italic>\n                    E555, a strain which expresses a\n                    <ns3:italic>B. pseudomallei<\/ns3:italic>\n                    -like capsular polysaccharide. Conclusion This is the first report of human infection with\n                    <ns3:italic>B. thailandensis<\/ns3:italic>\n                    in Laos, and the first report of any human infection with the\n                    <ns3:italic>B. thailandensis<\/ns3:italic>\n                    capsular variant. Due to the potential for laboratory tests to incorrectly identify this bacteria, staff in endemic areas for\n                    <ns3:italic>B. thailandensis<\/ns3:italic>\n                    and\n                    <ns3:italic>B. pseudomallei<\/ns3:italic>\n                    should be aware and ensure that appropriate confirmatory methods are used to differentiate between the species.\n                  <\/ns3:p>","DOI":"10.12688\/wellcomeopenres.22706.3","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T08:15:07Z","timestamp":1732781707000},"page":"421","update-policy":"https:\/\/doi.org\/10.12688\/wellcomeopenres.crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Case Report: Soft tissue infection with Burkholderia thailandensis capsular variant: case report from the Lao PDR"],"prefix":"10.12688","volume":"9","author":[{"given":"Souphaphone","family":"Vannachone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8801-5738","authenticated-orcid":false,"given":"Manophab","family":"Luangraj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9189-7244","authenticated-orcid":false,"given":"David","family":"Dance","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narisara","family":"Chantratita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natnaree","family":"Saiprom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rathanin","family":"Seng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0261-3233","authenticated-orcid":false,"given":"Sarunporn","family":"Tandhavanant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayaphet","family":"Rattanavong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1239-0782","authenticated-orcid":false,"given":"Andrew","family":"Simpson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8599-737X","authenticated-orcid":false,"given":"Tamalee","family":"Roberts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2024,11,28]]},"reference":[{"key":"ref-1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-8-r89","article-title":"Genomic 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Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/wellcomeopenresearch.org\/articles\/9-421\/v3\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/wellcomeopenresearch.org\/articles\/9-421\/v3\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/wellcomeopenresearch.org\/articles\/9-421\/v3\/iparadigms","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T08:15:12Z","timestamp":1732781712000},"score":1,"resource":{"primary":{"URL":"https:\/\/wellcomeopenresearch.org\/articles\/9-421\/v3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,28]]},"references-count":19,"URL":"https:\/\/doi.org\/10.12688\/wellcomeopenres.22706.3","relation":{"has-version":[{"id-type":"doi","id":"10.12688\/wellcomeopenres.22706.2","asserted-by":"subject"},{"id-type":"doi","id":"10.12688\/wellcomeopenres.22706.1","asserted-by":"subject"}],"has-review":[{"id-type":"doi","id":"10.21956\/wellcomeopenres.25902.r116835","asserted-by":"object"},{"id-type":"doi","id":"10.21956\/wellcomeopenres.25902.r113704","asserted-by":"object"},{"id-type":"doi","id":"10.21956\/wellcomeopenres.25902.r117093","asserted-by":"object"}]},"ISSN":["2398-502X"],"issn-type":[{"value":"2398-502X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,28]]},"assertion":[{"value":"Indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/9-421\/v3#article-reports","order":0,"name":"referee-status","label":"Referee status","group":{"name":"current-referee-status","label":"Current Referee Status"}},{"value":"10.21956\/wellcomeopenres.25008.r92509, Abdel Rahman Zueter, The Hashemite University, Zarqa, 31 Aug 2024, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/9-421\/v1#referee-response-92509","order":0,"name":"referee-response-92509","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Tamalee Roberts<\/b>; \n<i>Posted: 09 Sep 2024<\/i>; We are grateful to the reviewer for their thoughtful reading and comments. This is the first human case of BTCV of which we are aware of and therefore there are no similar cases.","URL":"https:\/\/wellcomeopenresearch.org\/articles\/9-421\/v3#referee-comment-5798","order":1,"name":"referee-comment-5798","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/wellcomeopenres.25008.r92503, Mohd Firdaus Raih, Universiti Kebangsaan Malaysia, Bangi, 05 Sep 2024, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/9-421\/v1#referee-response-92503","order":2,"name":"referee-response-92503","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"<b>Tamalee Roberts<\/b>; \n<i>Posted: 09 Sep 2024<\/i>; The authors thank the reviewer for their supportive comments.","URL":"https:\/\/wellcomeopenresearch.org\/articles\/9-421\/v3#referee-comment-5799","order":3,"name":"referee-comment-5799","label":"Referee Comment","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/wellcomeopenres.25442.r99762, Delaney Burnard, University of the Sunshine Coast (Ringgold ID: 5333), Sippy Downs, Queensland, 30 Sep 2024, version 2, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/9-421\/v2#referee-response-99762","order":4,"name":"referee-response-99762","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/wellcomeopenres.25442.r110287, Revathy Arushothy, Bacteriology Unit, Institute for Medical Research, National Institutes of Health,, Institute for Medical Research, SHAH ALAM, Selangor, 18 Nov 2024, version 2, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/9-421\/v2#referee-response-110287","order":5,"name":"referee-response-110287","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/wellcomeopenres.25442.r111302, Sylvia Chieng, Universiti Kebangsaan Malaysia, Bangi, 20 Nov 2024, version 2, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/9-421\/v2#referee-response-111302","order":6,"name":"referee-response-111302","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported by Wellcome [220211].","order":7,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}