{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T12:32:26Z","timestamp":1777984346432,"version":"3.51.4"},"reference-count":80,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["218771\/Z\/19\/Z"],"award-info":[{"award-number":["218771\/Z\/19\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011858","name":"African Academy of Sciences","doi-asserted-by":"publisher","award":["ARISE-PP-143"],"award-info":[{"award-number":["ARISE-PP-143"]}],"id":[{"id":"10.13039\/501100011858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["wellcomeopenresearch.org"],"crossmark-restriction":false},"short-container-title":["Wellcome Open Res"],"abstract":"<ns3:p>\n                    Background Mosquitoes harbor diverse and dynamic microbiota which plays a critical role in shaping their development, survival and vector competence. Many studies have focused on the fungal microbiota of mosquitoes to develop new mosquito control strategies and pathogen blocking tools. This review aims to synthesize current knowledge on the composition and functional roles of the mosquito fungal microbiota, highlighting its potential as a tool for innovative vector born disease control strategies. Methods This review was written in accordance with PRISMA guidelines. Studies on the fungal microbiota of species of the genera\n                    <ns3:italic>Aedes<\/ns3:italic>\n                    ,\n                    <ns3:italic>Anopheles<\/ns3:italic>\n                    , and\n                    <ns3:italic>Culex<\/ns3:italic>\n                    and their potential in vector control were searched for in two databases (PubMed, Google Scholar). The quality of the included studies was assessed using the Joanna Briggs Institute (JBI) critical appraisal tool. Fungal diversity was investigated according to geographical distribution, mosquito species, identification techniques, developmental stages, and digestive and reproductive organs. We are interested in the antivectorial potential of fungal species. Results A total of 32 studies were included in this review after screening 7,458 studies identified through search engines. Most studies on the fungal microbiota of species of the genera\n                    <ns3:italic>Aedes<\/ns3:italic>\n                    ,\n                    <ns3:italic>Anopheles<\/ns3:italic>\n                    , and\n                    <ns3:italic>Culex<\/ns3:italic>\n                    were conducted in the United States, Brazil, European countries (France, Italy), and Africa (Kenya). Several fungal genera were isolated in the microbiota, with a predominance in species of the genus\n                    <ns3:italic>Aedes<\/ns3:italic>\n                    , followed by\n                    <ns3:italic>Culex<\/ns3:italic>\n                    and\n                    <ns3:italic>Anopheles<\/ns3:italic>\n                    . The main identification technique used was molecular biology, sometimes combined with culture characteristics. Fungal species were isolated from digestive organs (intestines, intestinal diverticulum, crop), male and female genital organs, and whole specimens at the larval and adult stages. Three phyla (\n                    <ns3:italic>Ascomycota<\/ns3:italic>\n                    ,\n                    <ns3:italic>Basidiomycota<\/ns3:italic>\n                    , and\n                    <ns3:italic>Microsporidiomycota<\/ns3:italic>\n                    ) were predominant, with yeast-like, filamentous, and intracellular fungal species. Certain fungal strains inhibited the development of larval stages, while other fungal strains (\n                    <ns3:italic>Wickerhamomyces anomalus<\/ns3:italic>\n                    ,\n                    <ns3:italic>Microsporidia<\/ns3:italic>\n                    MB) reduced the vector capacity of\n                    <ns3:italic>An. stephensi<\/ns3:italic>\n                    and\n                    <ns3:italic>An. arabiensis<\/ns3:italic>\n                    species, respectively. Conclusion Mosquitoes harbor diverse fungal microbiota in their organs with antivectorial potential which remain largely unexplored to date. Future research should explore these interactions in greater depth, particularly in organs that have been little studied, such as the salivary glands and stomach, and in regions most affected by vector-borne diseases, such as Africa. These knowledges will enable their use in vector control.\n                  <\/ns3:p>","DOI":"10.12688\/wellcomeopenres.25128.1","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T10:04:06Z","timestamp":1771841046000},"page":"145","update-policy":"https:\/\/doi.org\/10.12688\/wellcomeopenres.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Fungal microbiota diversity in Aedes, Anopheles and Culex and their potential use as biological tools to control vector-borne diseases, a systematic review."],"prefix":"10.12688","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1502-3457","authenticated-orcid":false,"given":"Aicha Fatimata","family":"Sodr\u00e9","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0952-7310","authenticated-orcid":false,"given":"Doub\u00e9 Lucien","family":"Lamy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nouhoun","family":"Traore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdoulaye","family":"Diabat\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernand","family":"Sankara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2350-9339","authenticated-orcid":false,"given":"Etienne","family":"Bilgo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2560","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"ref-1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-024-06596-9","article-title":"Mosquito breeding water parameters are important determinants for Microsporidia MB in the aquatic stages of Anopheles species.","volume":"17","author":"E Akorli","year":"2024","journal-title":"Parasit Vectors."},{"key":"ref-2","doi-asserted-by":"publisher","DOI":"10.1038\/srep34084","article-title":"A natural Anopheles-associated Penicillium chrysogenum enhances mosquito susceptibility to Plasmodium infection.","volume":"6","author":"Y Angler\u00f3-Rodr\u00edguez","year":"2016","journal-title":"Sci Rep."},{"key":"ref-3","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.28844","article-title":"An Aedes aegypti-associated fungus increases susceptibility to dengue virus by modulating gut trypsin activity.","volume":"6","author":"Y Angler\u00f3-Rodr\u00edguez","year":"2017","journal-title":"eLife."},{"key":"ref-4","doi-asserted-by":"publisher","DOI":"10.1038\/srep01641","article-title":"Intra-specific diversity of Serratia marcescens in Anopheles mosquito midgut defines Plasmodium transmission capacity.","volume":"3","author":"H Bando","year":"2013","journal-title":"Sci Rep."},{"key":"ref-5","doi-asserted-by":"crossref","first-page":"1040-1046","DOI":"10.1093\/jmedent\/44.6.1040","article-title":"Microsporidiosis (Microsporidia: Culicosporidae) alters blood-feeding responses and DEET repellency in Aedes aegypti (Diptera: Culicidae).","volume":"44","author":"D Barnard","year":"2007","journal-title":"J Med Entomol."},{"key":"ref-6","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.68353","article-title":"Single mosquito metatranscriptomics identifies vectors, emerging pathogens and reservoirs in one assay.","volume":"10","author":"J Batson","year":"2021","journal-title":"eLife."},{"key":"ref-7","doi-asserted-by":"publisher","first-page":"748-751","DOI":"10.1126\/science.1236192","article-title":"Wolbachia invades Anopheles stephensi populations and induces refractoriness to Plasmodium infection.","volume":"340","author":"G Bian","year":"2013","journal-title":"Science."},{"key":"ref-8","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-2796-6","article-title":"Native entomopathogenic Metarhizium spp. from Burkina Faso and their virulence against the malaria vector Anopheles coluzzii and non-target insects.","volume":"11","author":"E Bilgo","year":"2018","journal-title":"Parasit Vectors."},{"key":"ref-9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000878","article-title":"Arbovirus detection in insect vectors by rapid, high-throughput pyrosequencing.","volume":"4","author":"K Bishop-Lilly","year":"2010","journal-title":"PLoS Negl Trop Dis."},{"key":"ref-11","doi-asserted-by":"publisher","first-page":"642-648","DOI":"10.1111\/1758-2229.12569","article-title":"Mosquitoes can harbour yeasts of clinical significance and contribute to their environmental dissemination.","volume":"9","author":"J Bozic","year":"2017","journal-title":"Environ Microbiol Rep."},{"key":"ref-12","doi-asserted-by":"publisher","first-page":"98-106","DOI":"10.1016\/j.jinsphys.2017.10.012","article-title":"Physiological characterization and regulation of the contractile properties of the mosquito ventral diverticulum (crop).","volume":"103","author":"T Calkins","year":"2017","journal-title":"J Insect Physiol."},{"key":"ref-13","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1157299","article-title":"Tripartite interactions comprising yeast-endobacteria systems in the gut of vector mosquitoes.","volume":"14","author":"A Cappelli","year":"2023","journal-title":"Front Microbiol."},{"key":"ref-14","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.621605","article-title":"Wickerhamomyces anomalus in mosquitoes: a promising yeast-based tool for the \u201csymbiotic control\u201d of mosquito-borne diseases.","volume":"11","author":"A Cappelli","year":"2021","journal-title":"Front Microbiol."},{"key":"ref-15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0095988","article-title":"A Wickerhamomyces anomalus killer strain in the malaria vector Anopheles stephensi.","volume":"9","author":"A Cappelli","year":"2014","journal-title":"PLoS One."},{"key":"ref-16","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3587-4","article-title":"Killer yeasts exert anti-plasmodial activities against the malaria parasite Plasmodium berghei in the vector mosquito Anopheles stephensi and in mice.","volume":"12","author":"A Cappelli","year":"2019","journal-title":"Parasit Vectors."},{"key":"ref-17","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2021.715759","article-title":"Emerging and re-emerging vector-borne infectious diseases and the challenges for control: a review.","volume":"9","author":"B Chala","year":"2021","journal-title":"Front Public Health."},{"key":"ref-18","doi-asserted-by":"publisher","first-page":"185","DOI":"10.3389\/fmicb.2015.00185","article-title":"RNA shotgun metagenomic sequencing of northern California (USA) mosquitoes uncovers viruses, bacteria, and fungi.","volume":"6","author":"J Chandler","year":"2015","journal-title":"Front Microbiol."},{"key":"ref-58","doi-asserted-by":"publisher","first-page":"352-359","DOI":"10.1590\/s1519-566x2009000300009","article-title":"Filamentous fungi associated with mosquito larvae (Diptera: Culicidae) in municipalities of the Brazilian Amazon.","volume":"38","author":"E da S Pereira","year":"2009","journal-title":"Neotrop Entomol."},{"key":"ref-19","doi-asserted-by":"publisher","first-page":"310","DOI":"10.3390\/pathogens9040310","article-title":"Mosquito-borne diseases emergence\/resurgence and how to effectively control it biologically.","volume":"9","author":"H Dahmana","year":"2020","journal-title":"Pathogens."},{"key":"ref-10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2020.107505","article-title":"Antifungal immune responses in mosquitoes (Diptera: Culicidae): a review.","volume":"178","author":"R de Bitencourt","year":"2021","journal-title":"J Invertebr Pathol."},{"key":"ref-20","doi-asserted-by":"publisher","first-page":"8088-8093","DOI":"10.1073\/pnas.1413835112","article-title":"Transmission potential of Rickettsia felis infection by Anopheles gambiae mosquitoes.","volume":"112","author":"C Dieme","year":"2015","journal-title":"Proc Natl Acad Sci U S A."},{"key":"ref-21","doi-asserted-by":"publisher","first-page":"29-54","DOI":"10.1016\/B978-0-12-811436-0.00002-2","article-title":"Arthropods: Definition and Medical Importance.","author":"G Duvallet","year":"2018"},{"key":"ref-22","doi-asserted-by":"publisher","first-page":"1074-1077","DOI":"10.1126\/science.1199115","article-title":"Development of transgenic fungi that kill human malaria parasites in mosquitoes.","volume":"331","author":"W Fang","year":"2011","journal-title":"Science."},{"key":"ref-23","doi-asserted-by":"publisher","first-page":"9047-9051","DOI":"10.1073\/pnas.0610451104","article-title":"Bacteria of the genus Asaia stably associate with Anopheles stephensi, an Asian malarial mosquito vector.","volume":"104","author":"G Favia","year":"2007","journal-title":"Proc Natl Acad Sci U S A."},{"key":"ref-24","doi-asserted-by":"publisher","DOI":"10.4178\/epih.e2018015","article-title":"Distribution and larval breeding habitats of Aedes mosquito species in residential areas of northwest Ethiopia.","volume":"40","author":"G Ferede","year":"2018","journal-title":"Epidemiol Health."},{"key":"ref-25","doi-asserted-by":"publisher","DOI":"10.3389\/ffunb.2022.918052","article-title":"Culex quinquefasciatus Holobiont: a fungal metagenomic approach.","volume":"3","author":"G Flores","year":"2022","journal-title":"Front Fungal Biol."},{"key":"ref-80","doi-asserted-by":"publisher","first-page":"243-294","DOI":"10.4000\/books.irdeditions.22040","article-title":"Culicinae (Diptera: Culicidae).","author":"D Fontenille","year":"2017"},{"key":"ref-26","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-69828-9","article-title":"Shifts in the microbiota associated with male mosquitoes (Aedes aegypti) exposed to an obligate gut fungal symbiont (Zancudomyces culisetae).","volume":"10","author":"J Frankel-Bricker","year":"2020","journal-title":"Sci Rep."},{"key":"ref-27","doi-asserted-by":"publisher","first-page":"e02334-19","DOI":"10.1128\/AEM.02334-19","article-title":"Influences of a prolific gut fungus (Zancudomyces culisetae) on larval and adult mosquito (Aedes aegypti)-associated microbiota.","volume":"86","author":"J Frankel-Bricker","year":"2020","journal-title":"Appl Environ Microbiol."},{"key":"ref-28","doi-asserted-by":"publisher","first-page":"1-6","DOI":"10.5152\/etd.2017.16067","article-title":"Culex (Culex) Pipiens mosquitoes carry and harbor pathogenic fungi during their developmental stages.","volume":"39","author":"F Galal","year":"2017","journal-title":"Erciyes Med J."},{"key":"ref-29","doi-asserted-by":"publisher","first-page":"98-111","DOI":"10.1016\/j.pt.2019.12.001","article-title":"Mosquito microbiota and implications for disease control.","volume":"36","author":"H Gao","year":"2020","journal-title":"Trends Parasitol."},{"key":"ref-30","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1208633","article-title":"The interplay between vector microbial community and pathogen transmission on the invasive Asian tiger mosquito, Aedes albopictus: current knowledge and future directions.","volume":"14","author":"M Garrido","year":"2023","journal-title":"Front Microbiol."},{"key":"ref-31","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-022-01325-9","article-title":"Mosquito sex and mycobiota contribute to fructose metabolism in the Asian tiger mosquito Aedes albopictus.","volume":"10","author":"M Gu\u00e9gan","year":"2022","journal-title":"Microbiome."},{"key":"ref-32","doi-asserted-by":"publisher","first-page":"628","DOI":"10.3390\/pathogens9080628","article-title":"Comparative analysis of the bacterial and fungal communities in the gut and the crop of Aedes albopictus mosquitoes: a preliminary study.","volume":"9","author":"M Gu\u00e9gan","year":"2020a","journal-title":"Pathogens."},{"key":"ref-33","doi-asserted-by":"publisher","first-page":"1193-1206","DOI":"10.1111\/1462-2920.14915","article-title":"Who is eating fructose within the Aedes albopictus gut microbiota?","volume":"22","author":"M Gu\u00e9gan","year":"2020b","journal-title":"Environ Microbiol."},{"key":"ref-35","doi-asserted-by":"publisher","first-page":"919-924","DOI":"10.1590\/s0074-02762007000800005","article-title":"First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): new perspectives for an insect-bacteria association.","volume":"102","author":"D Gusm\u00e3o","year":"2007","journal-title":"Mem Inst Oswaldo Cruz."},{"key":"ref-34","doi-asserted-by":"publisher","first-page":"275-281","DOI":"10.1016\/j.actatropica.2010.04.011","article-title":"Culture-dependent and culture-independent characterization of microorganisms associated with Aedes aegypti (Diptera: Culicidae) (L.) and dynamics of bacterial colonization in the midgut.","volume":"115","author":"D Gusm\u00e3o","year":"2010","journal-title":"Acta Trop."},{"key":"ref-36","doi-asserted-by":"publisher","DOI":"10.4324\/9781003056034-3","article-title":"The mosquito.","author":"F Hawkes","year":"2022"},{"key":"ref-37","doi-asserted-by":"publisher","DOI":"10.1186\/s42523-024-00298-4","article-title":"Interkingdom interactions shape the fungal microbiome of mosquitoes.","volume":"6","author":"S Hegde","year":"2024","journal-title":"Anim Microbiome."},{"key":"ref-38","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16121-y","article-title":"A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes.","volume":"11","author":"J Herren","year":"2020","journal-title":"Nat Commun."},{"key":"ref-39","doi-asserted-by":"publisher","first-page":"119-136","DOI":"10.1051\/jbio\/2019003","article-title":"Le microbiote de moustique et son influence sur la transmission vectorielle.","volume":"212","author":"K Heu","year":"2018","journal-title":"Biol Aujourdhui."},{"key":"ref-40","doi-asserted-by":"publisher","DOI":"10.3389\/fitd.2023.1230903","article-title":"Available evidence for mosquito-borne Francisella tularensis transmission is inconclusive.","volume":"4","author":"L Jonckers Nieboer","year":"2023","journal-title":"Front Trop Dis."},{"key":"ref-41","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-3920-y","article-title":"Habitat characteristics and insecticide susceptibility of Aedes aegypti in the Ifakara area, south-eastern Tanzania.","volume":"13","author":"N Kahamba","year":"2020","journal-title":"Parasit Vectors."},{"key":"ref-42","doi-asserted-by":"publisher","first-page":"104","DOI":"10.3390\/tropicalmed6020104","article-title":"Larvicidal activities against Aedes aegypti of supernatant and pellet fractions from cultured Bacillus spp. isolated from Amazonian microenvironments.","volume":"6","author":"R Katak","year":"2021","journal-title":"Trop Med Infect Dis."},{"key":"ref-43","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-024-06120-z","article-title":"Distribution of Culex pipiens life stages across urban green and grey spaces in Leiden, The Netherlands.","volume":"17","author":"L Krol","year":"2024","journal-title":"Parasit Vectors."},{"key":"ref-44","doi-asserted-by":"publisher","first-page":"290","DOI":"10.12688\/wellcomeopenres.21238.3","article-title":"Entomopathogenic fungi Metarhizium pingshaense increases susceptibility to insecticides in highly resistant malaria mosquitoes Anopheles coluzzii [version 3; peer review: 2 approved, 2 approved with reservations].","volume":"9","author":"D Lamy","year":"2025","journal-title":"Wellcome Open Res."},{"key":"ref-45","doi-asserted-by":"publisher","first-page":"e0095923","DOI":"10.1128\/aem.00959-23","article-title":"Introducing an environmental microbiome to axenic Aedes aegypti mosquitoes documents bacterial responses to a blood meal.","volume":"89","author":"J LaReau","year":"2023","journal-title":"Appl Environ Microbiol."},{"key":"ref-46","doi-asserted-by":"publisher","first-page":"242-250","DOI":"10.1016\/j.actatropica.2008.06.008","article-title":"Transstadial and horizontal transfer of bacteria within a colony of Anopheles gambiae (Diptera: Culicidae) and oviposition response to bacteria-containing water.","volume":"107","author":"J Lindh","year":"2008","journal-title":"Acta Trop."},{"key":"ref-47","doi-asserted-by":"publisher","first-page":"257-266","DOI":"10.1016\/j.funeco.2019.02.004","article-title":"Aedes albopictus mosquitoes host a locally structured mycobiota with evidence of reduced fungal diversity in invasive populations.","volume":"39","author":"P Luis","year":"2019","journal-title":"Fungal Ecol."},{"key":"ref-48","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-025-02099-6","article-title":"Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus.","volume":"13","author":"S Malassign\u00e9","year":"2025","journal-title":"Microbiome."},{"key":"ref-49","doi-asserted-by":"publisher","first-page":"564","DOI":"10.3390\/pathogens9070564","article-title":"Mosquito mycobiota: an overview of non-entomopathogenic fungal interactions.","volume":"9","author":"S Malassign\u00e9","year":"2020","journal-title":"Pathogens."},{"key":"ref-50","doi-asserted-by":"publisher","first-page":"492","DOI":"10.3389\/fmicb.2019.00492","article-title":"Aedes\u2013chikungunya virus interaction: key role of vector midguts microbiota and its saliva in the host infection.","volume":"10","author":"V Monteiro","year":"2019","journal-title":"Front Microbiol."},{"key":"ref-51","doi-asserted-by":"publisher","first-page":"4075-4090","DOI":"10.1111\/mec.13741","article-title":"Midgut fungal and bacterial microbiota of Aedes triseriatus and Aedes japonicus shift in response to La Crosse virus infection.","volume":"25","author":"E Muturi","year":"2016","journal-title":"Mol Ecol."},{"key":"ref-52","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0246452","article-title":"The fungus Leptosphaerulina persists in Anopheles gambiae and induces melanization.","volume":"16","author":"G Nattoh","year":"2021a","journal-title":"PLoS One."},{"key":"ref-53","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.647183","article-title":"Horizontal Transmission of the Symbiont Microsporidia MB in Anopheles arabiensis.","volume":"12","author":"G Nattoh","year":"2021b","journal-title":"Front Microbiol."},{"key":"ref-54","doi-asserted-by":"publisher","first-page":"30-39","DOI":"10.1016\/j.funeco.2015.10.001","article-title":"Yeasts and yeast-like fungi in tap water and groundwater, and their transmission to household appliances.","volume":"20","author":"M Novak Babi\u010d","year":"2016","journal-title":"Fungal Ecol."},{"key":"ref-55","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012340","article-title":"The Plasmodium transmission-blocking symbiont, Microsporidia MB, is vertically transmitted through Anopheles arabiensis germline stem cells.","volume":"20","author":"T Onchuru","year":"2024","journal-title":"PLoS Pathog."},{"key":"ref-56","doi-asserted-by":"publisher","DOI":"10.1186\/s13643-016-0384-4","article-title":"Rayyan\u2014a web and mobile app for systematic reviews.","volume":"5","author":"M Ouzzani","year":"2016","journal-title":"Syst Rev."},{"key":"ref-57","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.n71","article-title":"The PRISMA 2020 statement: an updated guideline for reporting systematic reviews.","volume":"372","author":"M Page","year":"2021","journal-title":"BMJ."},{"key":"ref-73","doi-asserted-by":"publisher","DOI":"10.1111\/1748-5967.12717","article-title":"A review of entomopathogenic fungi as a potential tool for mosquito vector control: a cost\u2010effective and environmentally friendly approach.","volume":"54","author":"V Perumal","year":"2024","journal-title":"Entomol Res."},{"key":"ref-59","doi-asserted-by":"publisher","first-page":"911-921","DOI":"10.1111\/j.1462-2920.2010.02395.x","article-title":"The yeast Wickerhamomyces anomalus (Pichia anomala) inhabits the midgut and reproductive system of the Asian malaria vector Anopheles stephensi.","volume":"13","author":"I Ricci","year":"2011a","journal-title":"Environ Microbiol."},{"key":"ref-60","doi-asserted-by":"publisher","first-page":"43-50","DOI":"10.1007\/s10482-010-9532-3","article-title":"Different mosquito species host Wickerhamomyces anomalus (Pichia anomala): perspectives on vector-borne diseases symbiotic control.","volume":"99","author":"I Ricci","year":"2011b","journal-title":"Antonie Van Leeuwenhoek."},{"key":"ref-61","doi-asserted-by":"publisher","first-page":"101-122","DOI":"10.1016\/B978-0-12-805350-8.00006-4","article-title":"The mosquito immune system and its interactions with the microbiota: implications for disease transmission.","volume":"1","author":"F Rodgers","year":"2017"},{"key":"ref-62","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-2784-x","article-title":"The tripartite interactions between the mosquito, its microbiota and Plasmodium.","volume":"11","author":"O Romoli","year":"2018","journal-title":"Parasit Vectors."},{"key":"ref-63","doi-asserted-by":"publisher","first-page":"81-93","DOI":"10.1590\/0074-02760160373","article-title":"Microbial control of arthropod-borne disease.","volume":"112","author":"M Salda\u00f1a","year":"2017","journal-title":"Mem Inst Oswaldo Cruz."},{"key":"ref-64","doi-asserted-by":"publisher","first-page":"332","DOI":"10.3390\/microorganisms10020332","article-title":"Influence of host blood meal source on gut microbiota of wild caught Aedes aegypti, a dominant arboviral disease vector.","volume":"10","author":"D Sarma","year":"2022","journal-title":"Microorganisms."},{"key":"ref-65","doi-asserted-by":"publisher","first-page":"20-34","DOI":"10.1038\/s41564-018-0214-7","article-title":"Vector biology meets disease control: using basic research to fight vector-borne diseases.","volume":"4","author":"W Shaw","year":"2019","journal-title":"Nat Microbiol."},{"key":"ref-66","doi-asserted-by":"publisher","first-page":"471-480","DOI":"10.1016\/j.aspen.2019.02.011","article-title":"Bacterial and eukaryote microbiomes of mosquito habitats in dengue-endemic southern Taiwan.","volume":"22","author":"M Shelomi","year":"2019","journal-title":"J Asia Pac Entomol."},{"key":"ref-67","doi-asserted-by":"publisher","first-page":"747-760","DOI":"10.1007\/s00248-015-0709-1","article-title":"Yeasts Associated with Culex pipiens and Culex theileri mosquito larvae and the effect of selected yeast strains on the ontogeny of Culex pipiens.","volume":"71","author":"A Steyn","year":"2016","journal-title":"Microb Ecol."},{"key":"ref-68","first-page":"36-40","article-title":"Study on fungal flora in the midgut of the larva and adult of the different populations of the Malaria vector Anopheles stephensi.","volume":"3","author":"L Tajedin","year":"2009","journal-title":"Iran J Arthropod Borne Dis."},{"key":"ref-69","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00646-21","article-title":"Host-environment interplay shapes fungal diversity in mosquitoes.","volume":"6","author":"P Tawidian","year":"2021","journal-title":"mSphere."},{"key":"ref-70","doi-asserted-by":"publisher","first-page":"1352-1368","DOI":"10.1002\/ece3.3676","article-title":"Mosquito vector\u2010associated microbiota: metabarcoding bacteria and eukaryotic symbionts across habitat types in Thailand endemic for dengue and other arthropod\u2010borne diseases.","volume":"8","author":"P Thongsripong","year":"2017","journal-title":"Ecol Evol."},{"key":"ref-71","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-87122-0","article-title":"Metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes.","volume":"11","author":"P Thongsripong","year":"2021","journal-title":"Sci Rep."},{"key":"ref-72","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-015-1059-7","article-title":"A yeast strain associated to Anopheles mosquitoes produces a toxin able to kill malaria parasites.","volume":"15","author":"M Valzano","year":"2016","journal-title":"Malar J."},{"key":"ref-74","doi-asserted-by":"publisher","first-page":"1399-1402","DOI":"10.1126\/science.aan5478","article-title":"Driving mosquito refractoriness to Plasmodium falciparum with engineered symbiotic bacteria.","volume":"357","author":"S Wang","year":"2017","journal-title":"Science."},{"key":"ref-75","doi-asserted-by":"publisher","first-page":"12734-12739","DOI":"10.1073\/pnas.1204158109","article-title":"Fighting malaria with engineered symbiotic bacteria from vector mosquitoes.","volume":"109","author":"S Wang","year":"2012","journal-title":"Proc Natl Acad Sci U S A."},{"key":"ref-76","article-title":"Evaluation of genetically modified mosquitoes for the control of vector-borne 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