{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T17:08:09Z","timestamp":1780506489043,"version":"3.54.1"},"reference-count":21,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2016,11,17]],"date-time":"2016-11-17T00:00:00Z","timestamp":1479340800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wellcome Trust","award":["WT109965MA"],"award-info":[{"award-number":["WT109965MA"]}]},{"name":"Wellcome Trust","award":["090532\/Z\/09\/Z"],"award-info":[{"award-number":["090532\/Z\/09\/Z"]}]},{"name":"Wellcome Trust","award":["WT091663MA"],"award-info":[{"award-number":["WT091663MA"]}]}],"content-domain":{"domain":["wellcomeopenresearch.org"],"crossmark-restriction":false},"short-container-title":["Wellcome Open Res"],"abstract":"<ns4:p>\n                    <ns4:bold>Background:<\/ns4:bold>\n                    Interleukin (IL)-27 is a member of the IL-6\/IL-12 family of cytokines. It is a potent cytokine, with potential antiviral impact, and has been shown to play a role in modulating functions of diverse cell types, including Th1, Th2, and NK and B cells, demonstrating both pro- and anti-inflammatory roles.\u00a0 In hepatocytes, it is capable of inducing signal transducer and activator of transcription (STAT)1, STAT3 and interferon-stimulated genes.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Methods:<\/ns4:bold>\n                    To address its role in viral hepatitis, the antiviral activity of IL-27 against hepatitis C virus (HCV) and hepatitis B virus (HBV) was tested\n                    <ns4:italic>in vitro<\/ns4:italic>\n                    using cell-culture-derived infectious HCV (HCVcc) cell culture system and the HepaRG HBV cell culture model. To further investigate the impact of IL-27 on hepatocytes, Huh7.5 cells were treated with IL-27 to analyse the differentially expressed genes by microarray analysis. Furthermore, by quantitative PCR, we analyzed the up-regulation of chemokine\n                    <ns4:italic>(CXCL)-10<\/ns4:italic>\n                    in response to IL-27.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Results:<\/ns4:bold>\n                    In both HCV and HBV infection models, we observed only a modest direct antiviral effect. Microarray analysis showed that the up-regulated genes mostly belonged to antigen presentation and DNA replication pathways, and involved strong up-regulation of\n                    <ns4:italic>CXCL-10<\/ns4:italic>\n                    , a gene associated with liver inflammation. Overall, gene set enrichment analysis showed a striking correlation of these genes with those up-regulated in response to related cytokines in diverse cell populations.\n                  <\/ns4:p>\n                  <ns4:p>\n                    <ns4:bold>Conclusion:<\/ns4:bold>\n                    <ns4:italic\/>\n                    Our data indicate that IL-27 can have a significant pro-inflammatory impact\n                    <ns4:italic>in vitro<\/ns4:italic>\n                    , although the direct antiviral effect is modest. It may have a potential impact on hepatocyte function, especially chemokine expression and antigen presentation.\n                  <\/ns4:p>","DOI":"10.12688\/wellcomeopenres.9917.1","type":"journal-article","created":{"date-parts":[[2016,11,17]],"date-time":"2016-11-17T05:05:20Z","timestamp":1479359120000},"page":"17","update-policy":"https:\/\/doi.org\/10.12688\/wellcomeopenres.crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Impact of IL-27 on hepatocyte antiviral gene expression and function"],"prefix":"10.12688","volume":"1","author":[{"given":"Narayan","family":"Ramamurthy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Boninsegna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Adams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natasha","family":"Sahgal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Lockstone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilair","family":"Baban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Marchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4307-9161","authenticated-orcid":false,"given":"Paul","family":"Klenerman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2560","published-online":{"date-parts":[[2016,11,17]]},"reference":[{"key":"ref-1","doi-asserted-by":"publisher","first-page":"4886-90","DOI":"10.4049\/jimmunol.170.10.4886","article-title":"Cutting 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Lloyd, Viral immunology Systems Program, Kirby Institute, University of New South Wales, Sydney, NSW, Australia, 05 Dec 2016, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/1-17\/v1#referee-response-17986","order":1,"name":"referee-response-17986","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"10.21956\/wellcomeopenres.10688.r18168, Ruth Broering, Department of Gastroenterology and Hepatology, University Hospital of Essen, University of Duisburg-Essen, Essen, Germany, 19 Dec 2016, version 1, indexed","URL":"https:\/\/wellcomeopenresearch.org\/articles\/1-17\/v1#referee-response-18168","order":2,"name":"referee-response-18168","label":"Referee Report","group":{"name":"article-reports","label":"Article Reports"}},{"value":"This work was supported by the Wellcome Trust [WT091663MA, WT109965MA, and 090532\/Z\/09\/Z]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":3,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}