{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T06:44:02Z","timestamp":1775630642799,"version":"3.50.1"},"reference-count":26,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018693","name":"Horizon Europe Framework Programme","doi-asserted-by":"publisher","award":["101059492"],"award-info":[{"award-number":["101059492"]}],"id":[{"id":"10.13039\/100018693","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agence nationale de la recherche","award":["ANR-22-EXAT-0003-SEQ-Sea"],"award-info":[{"award-number":["ANR-22-EXAT-0003-SEQ-Sea"]}]},{"name":"Swiss State Secretariat for Education, Research and Innovation","award":["22.00173"],"award-info":[{"award-number":["22.00173"]}]},{"name":"Swiss State Secretariat for Education, Research and Innovation","award":["24.00054"],"award-info":[{"award-number":["24.00054"]}]},{"DOI":"10.13039\/100014013","name":"UK Research and Innovation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100014013","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Agence nationale de la recherche","award":["ANR-10-INBS-09-08"],"award-info":[{"award-number":["ANR-10-INBS-09-08"]}]}],"content-domain":{"domain":["open-research-europe.ec.europa.eu"],"crossmark-restriction":false},"short-container-title":["Open Res Europe"],"abstract":"<ns3:p>\n                    <ns3:italic>Carlina diae<\/ns3:italic>\n                    (\n                    <ns3:italic>Asteraceae<\/ns3:italic>\n                    ) is a rare Cretan endemic plant confined to limestone cliff crevices in four locations of eastern Crete, as well as Dia and the Dionysades islets. It is the only species of the\n                    <ns3:italic>Carlina<\/ns3:italic>\n                    genus lacking spiny structures, a trait that, together with its placement in the ancestral subgenus\n                    <ns3:italic>Lyrolepis<\/ns3:italic>\n                    , highlights its significance as a Tertiary relict and a representative of an ancient evolutionary lineage. Fewer than 1,000 mature individuals persist in fragmented subpopulations, each typically numbering fewer than 250 plants, all within Natura 2000 sites, which are protected areas covering Europe's most valuable and threatened species and habitats. The species is currently classified as Endangered on the IUCN Red List. Despite its restricted range,\n                    <ns3:italic>C. diae<\/ns3:italic>\n                    contributes substantially to ecosystem functioning. It can stabilize shallow soils on rocky slopes and support the integrity of fragile montane habitats. Flowering during the dry summer months, its capitula provides critical nectar and pollen resources for pollinators, including solitary bees, thereby sustaining biodiversity during periods of resource scarcity. Unlike several species of the\n                    <ns3:italic>Asteraceae<\/ns3:italic>\n                    family with ornamental value,\n                    <ns3:italic>C. diae<\/ns3:italic>\n                    is not cultivated and is poorly represented in ex-situ collections, underscoring the urgency of conservation measures. Genomic studies, including full genome annotation, are expected to play a pivotal role in safeguarding its genetic diversity, informing effective management strategies, and improving understanding of its unique evolutionary history. The entirety of the genome sequence was assembled into 10 contiguous chromosomal pseudomolecules, 2 mitochondrial genomes, and one plastid genome. This chromosome-level assembly encompasses 4.2 Gb, composed of 456 contigs and 102 scaffolds, with contig and scaffold N50 values of 23.5 Mb and 416.6 Mb, respectively.\n                  <\/ns3:p>","DOI":"10.12688\/openreseurope.22092.1","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T11:54:08Z","timestamp":1770119648000},"page":"34","update-policy":"https:\/\/doi.org\/10.12688\/openreseurope.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["ERGA-BGE reference genome of Carlina diae, an endemic spineless thistle of Crete, Greece"],"prefix":"10.12688","volume":"6","author":[{"given":"Eleftheria","family":"Antaloudaki","sequence":"first","affiliation":[{"name":"Natural History Museum of Crete, School of Sciences and Engineering, Knossos Avenue Heraklion GR-71409 University of Crete"}]},{"given":"Danae","family":"Karakasi","sequence":"additional","affiliation":[{"name":"Natural History Museum of Crete, School of Sciences and Engineering, Knossos Avenue Heraklion GR-71409 University of Crete"},{"name":"Department of Biology, School of Sciences and Engineering, Vassilika Vouton Heraklion GR-70013 University of Crete"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6291-9245","authenticated-orcid":false,"given":"Manos","family":"Stratakis","sequence":"additional","affiliation":[{"name":"Natural History Museum of Crete, School of Sciences and Engineering, Knossos Avenue Heraklion GR-71409 University of Crete"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5434-0550","authenticated-orcid":false,"given":"Eleftherios","family":"Bitzilekis","sequence":"additional","affiliation":[{"name":"Natural History Museum of Crete, School of Sciences and Engineering, Knossos Avenue Heraklion GR-71409 University of Crete"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5067-9600","authenticated-orcid":false,"given":"Petros","family":"Lymberakis","sequence":"additional","affiliation":[{"name":"Natural History Museum of Crete, School of Sciences and Engineering, Knossos Avenue Heraklion GR-71409 University of Crete"}]},{"given":"Nikolaos","family":"Poulakakis","sequence":"additional","affiliation":[{"name":"Natural History Museum of Crete, School of Sciences and Engineering, Knossos Avenue Heraklion GR-71409 University of Crete"},{"name":"Department of Biology, School of Sciences and Engineering, Vassilika Vouton Heraklion GR-70013 University of Crete"}]},{"given":"Tereza","family":"Manousaki","sequence":"additional","affiliation":[{"name":"Crete 70014 Institute of Marine Biology Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1284-3115","authenticated-orcid":false,"given":"Astrid","family":"B\u00f6hne","sequence":"additional","affiliation":[{"name":"Leibniz Institute for the Analysis of Biodiversity Change, Adenauerallee 127 Bonn 53113 Museum Koenig Bonn"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1374-4474","authenticated-orcid":false,"given":"Rita","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Leibniz Institute for the Analysis of Biodiversity Change, Adenauerallee 127 Bonn 53113 Museum Koenig Bonn"}]},{"given":"Rosa","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Metazoa Phylogenomics Lab, Passeig mar\u00edtim de la Barceloneta 37-49 Barcelona 08003 Institute for Evolutionary Biology (CSIC-UPF)."}]},{"given":"Nuria","family":"Escudero","sequence":"additional","affiliation":[{"name":"Metazoa Phylogenomics Lab, Passeig mar\u00edtim de la Barceloneta 37-49 Barcelona 08003 Institute for Evolutionary Biology (CSIC-UPF)."}]},{"name":"Genoscope Sequencing Team","sequence":"additional","affiliation":[]},{"given":"Alice","family":"Moussy","sequence":"additional","affiliation":[{"name":"Genoscope, Institut Fran\u00e7ois Jacob, CEA, CNRS, Univ Evry Evry 91057 Universit\u00e9 Paris-Saclay"}]},{"given":"Corinne","family":"Cruaud","sequence":"additional","affiliation":[{"name":"Genoscope, Institut Fran\u00e7ois Jacob, CEA, CNRS, Univ Evry Evry 91057 Universit\u00e9 Paris-Saclay"}]},{"given":"Karine","family":"Labadie","sequence":"additional","affiliation":[{"name":"Genoscope, Institut Fran\u00e7ois Jacob, CEA, CNRS, Univ Evry Evry 91057 Universit\u00e9 Paris-Saclay"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9164-3311","authenticated-orcid":false,"given":"Lola","family":"Demirdjian","sequence":"additional","affiliation":[{"name":"G\u00e9nomique M\u00e9tabolique, Genoscope, Institut Fran\u00e7ois Jacob, CEA, CNRS, Univ Evry Evry 91057 Universit\u00e9 Paris-Saclay"}]},{"given":"Patrick","family":"Wincker","sequence":"additional","affiliation":[{"name":"G\u00e9nomique M\u00e9tabolique, Genoscope, Institut Fran\u00e7ois Jacob, CEA, CNRS, Univ Evry Evry 91057 Universit\u00e9 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co-funded by the Swiss State Secretariat for Education, Research and Innovation (SERI) under contract numbers 22.00173 and 24.00054; and by the UK Research and Innovation (UKRI) under the Department for Business, Energy and Industrial Strategy\u2019s Horizon Europe Guarantee Scheme. This work was supported by the Genoscope, the Commissariat \u00e0 l'Energie Atomique et aux \u00c9nergies Alternatives (CEA), France G\u00e9nomique (ANR-10-INBS-09-08) and the exploratory research programme \u201cATLASea: Atlas of marine genomes\u201d and its targeted project SEQ-Sea (ANR-22-EXAT-0003-SEQ-Sea). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":3,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}