{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T20:41:38Z","timestamp":1769200898254,"version":"3.49.0"},"reference-count":36,"publisher":"F1000 Research Ltd","license":[{"start":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:00:00Z","timestamp":1769126400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["220540"],"award-info":[{"award-number":["220540"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Technology Agency of the Czech Republic","award":["SS07010197"],"award-info":[{"award-number":["SS07010197"]}]},{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"crossref","award":["2-35084J"],"award-info":[{"award-number":["2-35084J"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"crossref","award":["202869"],"award-info":[{"award-number":["202869"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["wellcomeopenresearch.org"],"crossmark-restriction":false},"short-container-title":["Wellcome Open Res"],"abstract":"<ns3:p>\n                    We present a genome assembly from a female specimen of\n                    <ns3:italic>Brintesia circe<\/ns3:italic>\n                    (Great Banded Grayling; Arthropoda; Insecta; Lepidoptera; Nymphalidae). The assembly contains two haplotypes with total lengths of 583.12 megabases and 533.70 megabases. Most of haplotype 1 (99.54%) is scaffolded into 30 chromosomal pseudomolecules, including the W and Z sex chromosomes. Haplotype 2 was assembled to scaffold level. The mitochondrial genome has also been assembled, with a length of 15.2 kilobases. This work is part of Project Psyche, a collaborative programme generating genomes for European butterflies and moths.\n                  <\/ns3:p>","DOI":"10.12688\/wellcomeopenres.25785.1","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T17:29:07Z","timestamp":1769189347000},"page":"59","update-policy":"https:\/\/doi.org\/10.12688\/wellcomeopenres.crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["The genome sequence of the Great Banded Grayling, Brintesia circe (Denis &amp; Schifferm\u00fcller), 1776 (Lepidoptera: Nymphalidae)"],"prefix":"10.12688","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0236-5110","authenticated-orcid":false,"given":"Yannick","family":"Chittaro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0686-3961","authenticated-orcid":false,"given":"Daniel","family":"Linke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2253-2556","authenticated-orcid":false,"given":"Kay","family":"Lucek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3971-4372","authenticated-orcid":false,"given":"Charlotte J.","family":"Wright","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joana 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Mestdagh","year":"2024","journal-title":"Ferrantia."},{"key":"ref-25","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14998-3","article-title":"GenomeScope 2.0 and Smudgeplot for reference-free profiling of polyploid genomes.","volume":"11","author":"T Ranallo-Benavidez","year":"2020","journal-title":"Nat Commun."},{"key":"ref-26","doi-asserted-by":"publisher","first-page":"1665-1680","DOI":"10.1016\/j.cell.2014.11.021","article-title":"A 3D map of the human genome at kilobase resolution reveals principles of chromatin looping.","volume":"159","author":"S Rao","year":"2014","journal-title":"Cell."},{"key":"ref-27","first-page":"51","article-title":"Verbreitungsatlas der Tagfalter und Widderchen Deutschlands.","volume":"50","author":"R Reinhardt","year":"2020","journal-title":"Hesperia."},{"key":"ref-28","doi-asserted-by":"publisher","first-page":"737-746","DOI":"10.1038\/s41586-021-03451-0","article-title":"Towards complete and error-free genome assemblies of all vertebrate 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Uliano-Silva","year":"2023","journal-title":"BMC Bioinformatics."},{"key":"ref-33","doi-asserted-by":"publisher","first-page":"314-324","DOI":"10.1109\/IPDPS.2019.00041","article-title":"Efficient architecture-aware acceleration of BWA-MEM for multicore systems.","author":"M Vasimuddin","year":"2019"},{"key":"ref-34","doi-asserted-by":"publisher","first-page":"777-790","DOI":"10.1038\/s41559-024-02329-4","article-title":"Comparative genomics reveals the dynamics of chromosome evolution in Lepidoptera.","volume":"8","author":"C Wright","year":"2024","journal-title":"Nat Ecol Evol."},{"key":"ref-35","first-page":"121-126","article-title":"Pierwsze stwierdzenie skalnika prozerpiny Brintesia circe (Fabricius, 1775) (Lepidoptera: Nymphalidae) w polskich Sudetach.","volume":"24","author":"K Zaj\u0105c","year":"2022","journal-title":"Przyroda Sudet\u00f3w."},{"key":"ref-36","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btac808","article-title":"YaHS: Yet another Hi-C Scaffolding tool.","volume":"39","author":"C Zhou","year":"2023","journal-title":"Bioinformatics."}],"container-title":["Wellcome Open 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DL received funding from Czech Science Foundation (GACR) grant 22-35084J and Technology Agency of the Czech Republic (TACR) grant SS07010197. KL was funded by Swiss National Science Foundation (SNSF) Grant ID 202869. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.","order":0,"name":"grant-information","label":"Grant Information"},{"value":"This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.","order":0,"name":"copyright-info","label":"Copyright"}]}}