{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T19:44:40Z","timestamp":1787600680835,"version":"build-2736575974"},"reference-count":40,"publisher":"Yeungnam University College of Medicine","funder":[{"name":"Chungnam RISE Center"},{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chungchengnam-do, Republic of Korea","award":["2025-RISE-12-003"],"award-info":[{"award-number":["2025-RISE-12-003"]}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2024-00346990"],"award-info":[{"award-number":["RS-2024-00346990"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["e-jyms.org"],"crossmark-restriction":true},"short-container-title":["J Yeungnam Med Sci"],"abstract":"<jats:p>Background: Isoquercitrin (IQ), a flavonoid with anticancer, antioxidant, anti-inflammatory, and antiallergic properties, exhibits dual effects on B16F10 melanoma cells by suppressing cell viability and inducing hyperpigmentation. This study investigated the molecular mechanisms underlying IQ-induced melanogenesis in vitro and in vivo, highlighting the potential of IQ in the treatment of vitiligo.Methods: B16F10 melanoma cells and zebrafish were treated with various IQ concentrations and melanogenesis was evaluated using western blotting, a tyrosinase activity assay, and microscopic observation. In addition, signaling pathway modulators and IQ were used to confirm the signaling pathways involved in IQ-induced melanogenesis.Results: IQ treatment of B16F10 melanoma cells enhanced tyrosinase activity and upregulated melanogenesis-associated proteins, including microphthalmia-associated transcription factor and tyrosinase, via the p38 and protein kinase A (PKA)\/cyclic AMP response element-binding protein (CREB) signaling pathways. Cotreatment with pathway-specific inhibitors (SB203580 for p38 and H89 for PKA\/CREB) attenuated melanogenesis, whereas forskolin (a PKA\/CREB activator) enhanced melanogenesis, confirming the involvement of these signaling pathways. In zebrafish, IQ treatment significantly increased melanophore production in the dorsal region following hypopigmentation induction with 1-phenyl-2-thiourea. Similar to that in B16F10 melanoma cells, SB203580 and H89 suppressed IQ-induced pigmentation, whereas forskolin enhanced it.Conclusion: These findings demonstrate that IQ promotes melanogenesis in vitro and in vivo through activation of the p38 and PKA\/CREB signaling pathways, supporting its potential as a therapeutic agent for vitiligo.<\/jats:p>","DOI":"10.12701\/jyms.2025.42.69","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T05:37:51Z","timestamp":1762321071000},"page":"69","update-policy":"https:\/\/doi.org\/10.12701\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Isoquercitrin induces melanogenesis in B16F10 melanoma cells and zebrafish via the p38 and PKA\/CREB signaling pathways: an experimental study"],"prefix":"10.12701","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4802-2897","authenticated-orcid":true,"given":"Jun 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