{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T16:43:31Z","timestamp":1770741811167,"version":"3.49.0"},"reference-count":0,"publisher":"H1 Connect","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Fac Rev"],"abstract":"<jats:p>While the human genome represents the most accurate vertebrate reference assembly to date, it still contains numerous gaps, including centromeric and other large repeat-containing regions \u2013 often termed the \u201cdark side\u201d of the genome \u2013 many of which are of fundamental biological importance. Miga et al. present the first gapless assembly of the human X chromosome, with the help of ultra-long-read nanopore reads generated for the haploid complete hydatidiform mole (CHM13) genome. They reconstruct the ~3.1 megabase centromeric satellite DNA array and map DNA methylation patterns across complex tandem repeats and satellite arrays. This Telomere-to-Telomere assembly provides a superior human X chromosome reference enabling future sex-determination and X-linked disease research, and provides a path towards finishing the entire human genome sequence.<\/jats:p>","DOI":"10.12703\/r-01-000001","type":"journal-article","created":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T14:37:37Z","timestamp":1628519857000},"source":"Crossref","is-referenced-by-count":2,"title":["The X Chromosome from Telomere to Telomere: Key Achievements and Future Opportunities"],"prefix":"10.12703","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8052-7117","authenticated-orcid":false,"given":"Edith","family":"Heard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander D","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2798-3794","authenticated-orcid":false,"given":"Jan O","family":"Korbel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7317-6662","authenticated-orcid":false,"given":"Charles","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0784-7987","authenticated-orcid":false,"given":"Michael P","family":"Snyder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7902-3605","authenticated-orcid":false,"given":"David","family":"Sturgill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4950","published-online":{"date-parts":[[2021,7,30]]},"container-title":["Faculty Reviews"],"original-title":[],"deposited":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T14:37:38Z","timestamp":1628519858000},"score":1,"resource":{"primary":{"URL":"https:\/\/facultyopinions.com\/faculty-reviews\/landmarks\/10-63"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,30]]},"references-count":0,"URL":"https:\/\/doi.org\/10.12703\/r-01-000001","relation":{},"ISSN":["2732-432X"],"issn-type":[{"value":"2732-432X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,30]]}}}