{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T18:23:27Z","timestamp":1793125407705,"version":"4.5.0"},"reference-count":54,"publisher":"Medical Association of Nippon Medical School","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Nippon Med Sch"],"published-print":{"date-parts":[[2022,6,25]]},"DOI":"10.1272\/jnms.jnms.2022_89-307","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T22:11:20Z","timestamp":1656367880000},"page":"316-327","source":"Crossref","is-referenced-by-count":11,"title":["Moderate-Intensity Exercise Improves Endothelial Function by Altering Gut Microbiome Composition in Rats Fed a High-Fat Diet"],"prefix":"10.1272","volume":"89","author":[{"given":"Honggang","family":"Yin","sequence":"first","affiliation":[{"name":"School of Kinesiology, Shanghai University of Sport"},{"name":"Guangdong Provincial Key Laboratory of Sports and Health Promotion, Scientific Research Center, Guangzhou Sport University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhao","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Sports and Health Promotion, Scientific Research Center, Guangzhou Sport University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Kinesiology, Shanghai University of Sport"},{"name":"Department of Sports and Health, Guangzhou Sport University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"21","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1. Virdis A, Masi S, Colucci R, et al. Microvascular endothelial dysfunction in patients with obesity. Curr Hypertens Rep [Internet]. 2019 Apr 4;21 (4):32. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30949772","DOI":"10.1007\/s11906-019-0930-2"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Reho JJ, Rahmouni K. Oxidative and inflammatory signals in obesity-associated vascular abnormalities. Clin Sci [Internet]. 2017 Jul 15;131 (14):1689-700. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28667067","DOI":"10.1042\/CS20170219"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3. Fiuza-Luces C, Garatachea N, Berger NA, Lucia A. Exercise is the real polypill. Physiology [Internet]. 2013 Sep;28 (5):330-58. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23997192","DOI":"10.1152\/physiol.00019.2013"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Canfora EE, Meex RCR, Venema K, Blaak EE. Gut microbial metabolites in obesity, NAFLD and T2DM. Nat Rev Endocrinol [Internet]. 2019 May;15 (5):261-73. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30670819","DOI":"10.1038\/s41574-019-0156-z"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5. Baothman OA, Zamzami MA, Taher I, Abubaker J, Abu-Farha M. The role of gut microbiota in the development of obesity and diabetes. Lipids Health Dis [Internet]. 2016 Jun 18;15:108. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27317359","DOI":"10.1186\/s12944-016-0278-4"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Zhong X, Harrington J, Millar S, Perry I, O&apos;Toole P, Phillips C. Gut microbiota associations with metabolic health and obesity status in older adults. Nutrients. 2020 Aug;12 (8):2364.","DOI":"10.3390\/nu12082364"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Daniel H, Gholami AM, Berry D, et al. High-fat diet alters gut microbiota physiology in mice. ISME J [Internet]. 2014 Feb;8 (2):295-308. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24030595","DOI":"10.1038\/ismej.2013.155"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. Wan Y, Wang FL, Yuan JH, et al. Effects of dietary fat on gut microbiota and faecal metabolites, and their relationship with cardiometabolic risk factors-a 6-month randomised controlled-feeding trial. Gut. 2019 Aug;68 (8):1417-29.","DOI":"10.1136\/gutjnl-2018-317609"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Backhed F, Ding H, Wang T, et al. The gut microbiota as an environmental factor that regulates fat storage. Proc Natl Acad Sci U S A [Internet]. 2004 Nov 2;101 (44):15718-23. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/15505215","DOI":"10.1073\/pnas.0407076101"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Nirmalkar K, Murugesan S, Pizano-Zarate ML, et al. Gut microbiota and endothelial dysfunction markers in obese mexican children and adolescents. Nutrients [Internet]. 2018 Dec 19;10 (12):2009. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30572569","DOI":"10.3390\/nu10122009"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11. Menni C, Lin C, Cecelja M, et al. Gut microbial diversity is associated with lower arterial stiffness in women. Eur Heart J [Internet]. 2018 Jul 1;39 (25):2390-7. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29750272","DOI":"10.1093\/eurheartj\/ehy226"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12. Mitchell CM, Davy BM, Hulver MW, Neilson AP, Bennett BJ, Davy KP. Does exercise alter gut microbial composition?-a systematic review. Med Sci Sports Exerc. 2018 Jan;51 (1):160-7.","DOI":"10.1249\/MSS.0000000000001760"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. O&apos;Sullivan O, Cronin O, Clarke SF, et al. Exercise and the microbiota. Gut microbes. 2015 Mar;6 (2):131-6.","DOI":"10.1080\/19490976.2015.1011875"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. Jang LG, Choi G, Kim SW, Kim BY, Lee S, Park H. The combination of sport and sport-specific diet is associated with characteristics of gut microbiota: an observational study. J Int Soc Sports Nutr [Internet]. 2019 May 3;16 (1):21. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/31053143","DOI":"10.1186\/s12970-019-0290-y"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Allen JM, Mailing LJ, Niemiro GM, et al. Exercise alters gut microbiota composition and function in lean and obese humans. Med Sci Sports Exerc [Internet]. 2018 Apr;50 (4):747-57. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29166320","DOI":"10.1249\/MSS.0000000000001495"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16. Clarke SF, Murphy EF, O&apos;Sullivan O, et al. Exercise and associated dietary extremes impact on gut microbial diversity. Gut [Internet]. 2014 Dec;63 (12):1913-20. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25021423","DOI":"10.1136\/gutjnl-2013-306541"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17. Evans CC, LePard KJ, Kwak JW, et al. Exercise prevents weight gain and alters the gut microbiota in a mouse model of high fat diet-induced obesity. PLoS One [Internet]. 2014 Mar;9 (3):e92193. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24670791","DOI":"10.1371\/journal.pone.0092193"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18. Keohane DM, Woods T, O&apos;Connor P, et al. Four men in a boat: Ultra-endurance exercise alters the gut microbiome. J Sci Med Sport [Internet]. 2019 Apr 18;22 (9):1059-64. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/31053425","DOI":"10.1016\/j.jsams.2019.04.004"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19. Denou E, Marcinko K, Surette MJ, Steinberg GR, Schertzer JD. High-intensity exercise training increases the diversity and metabolic capacity of the mouse distal gut microbiota during diet-induced obesity. Am J Physiol Endocrinol Metab. 2016 Jun;310 (11):E982-93.","DOI":"10.1152\/ajpendo.00537.2015"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20. Munukka E, Ahtiainen JP, Puigbo P, et al. Six-week endurance exercise alters gut metagenome that is not reflected in systemic metabolism in over-weight women. Front Microbiol [Internet]. 2018 Oct;9:2323. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30337914","DOI":"10.3389\/fmicb.2018.02323"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21. Liao J, Yin H, Huang J, Hu M. Dysfunction of PVAT in mesenteric artery is restored by aerobic exercise in high-fat diet induced obesity. Clin Exp Pharmacol Physiol. 2021 May;48 (5):697-703.","DOI":"10.1111\/1440-1681.13404"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22. Huang J, Zhang H, Tan X, Hu M, Shen B. Exercise restores impaired endothelium-derived hyperpolarizing factor-mediated vasodilation in aged rat aortic arteries via the TRPV4-K<sub>Ca<\/sub>2.3 signaling complex. Clin Interv Aging. 2019 Sep;14:1579-87.","DOI":"10.2147\/CIA.S220283"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23. Ye L, Xu M, Hu M, et al. TRPV4 is involved in irisin-induced endothelium-dependent vasodilation. Biochem Biophys Res Commun [Internet]. 2018 Jan 1;495 (1):41-5. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29097199","DOI":"10.1016\/j.bbrc.2017.10.160"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24. Fadrosh D, Ma B, Gajer P, et al. An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform. Microbiome. 2014 Feb;2 (1):6.","DOI":"10.1186\/2049-2618-2-6"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25. Caporaso JG, Kuczynski J, Stombaugh J, et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods [Internet]. 2010 May;7 (5):335-6. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/20383131","DOI":"10.1038\/nmeth.f.303"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26. Schloss PD, Westcott SL, Ryabin T, et al. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol [Internet]. 2009 Dec;75 (23):7537-41. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19801464","DOI":"10.1128\/AEM.01541-09"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27. White J, Nagarajan N, Pop M. Statistical methods for detecting differentially abundant features in clinical metagenomic samples. PLoS Comput Biol. 2009 Apr;5 (4):e1000352.","DOI":"10.1371\/journal.pcbi.1000352"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28. Segata N, Izard J, Waldron L, et al. Metagenomic biomarker discovery and explanation. Genome Biol. 2011 Jun;12 (6):R60.","DOI":"10.1186\/gb-2011-12-6-r60"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29. Faust K, Raes J. CoNet app: inference of biological association networks using Cytoscape. F1000Res. 2016 Jun;5:1519.","DOI":"10.12688\/f1000research.9050.2"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30. Huang JH, He GW, Xue HM, et al. TRPC3 channel contributes to nitric oxide release: significance during normoxia and hypoxia-reoxygenation. Cardiovasc Res [Internet]. 2011 Aug 1;91 (3):472-82. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21493700","DOI":"10.1093\/cvr\/cvr102"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31. Ratajczak M, Skrypnik D, Bogdanski P, et al. Effects of endurance and endurance-strength training on endothelial function in women with obesity: a randomized trial. Int J Environ Res Public Health [Internet]. 2019 Nov 5;16 (21):4291. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/31694237","DOI":"10.3390\/ijerph16214291"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32. Tomada I, Tomada N, Almeida H, Neves D. Energy restriction and exercise modulate angiopoietins and vascular endothelial growth factor expression in the cavernous tissue of high-fat diet-fed rats. Asian J Androl [Internet]. 2012 Jul;14 (4):635-42. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22138901","DOI":"10.1038\/aja.2011.131"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33. Gomes JL, Fernandes T, Soci UP, et al. Obesity downregulates microRNA-126 inducing capillary rarefaction in skeletal muscle: effects of aerobic exercise training. Oxid Med Cell Longev [Internet]. 2017;2017:2415246 Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28367267","DOI":"10.1155\/2017\/2415246"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34. Mayengbam S, Mickiewicz B, Trottier S, et al. Distinct gut microbiota and serum metabolites in response to weight loss induced by either dairy or exercise in a rodent model of obesity. J Proteome Res. 2019 Nov;18 (11):3867-75.","DOI":"10.1021\/acs.jproteome.9b00304"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35. Ribeiro FM, Ribeiro CFA, Garcia ACM, et al. Limited effects of low-to-moderate aerobic exercise on the gut microbiota of mice subjected to a high-fat diet. Nutrients [Internet]. 2019 Jan 11;11 (1):149. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30641996.","DOI":"10.3390\/nu11010149"},{"key":"36","doi-asserted-by":"crossref","unstructured":"36. Leigh S, Kaakoush N, Escorihuela R, Westbrook R, Morris M. Treadmill exercise has minimal impact on obesogenic diet-related gut microbiome changes but alters adipose and hypothalamic gene expression in rats. Nutr Metab. 2020 Aug;17:71.","DOI":"10.1186\/s12986-020-00492-6"},{"key":"37","doi-asserted-by":"crossref","unstructured":"37. Clauss M, G\u00e9rard P, Mosca A, Leclerc M. Interplay between exercise and gut microbiome in the context of human health and performance. Front Nutr. 2021 Jun;8:637010.","DOI":"10.3389\/fnut.2021.637010"},{"key":"38","doi-asserted-by":"crossref","unstructured":"38. Ley RE, Turnbaugh PJ, Klein S, Gordon JI. Human gut microbes associated with obesity. Nature. 2006 Dec;444 (7122):1022-3.","DOI":"10.1038\/4441022a"},{"key":"39","doi-asserted-by":"crossref","unstructured":"39. Magne F, Gotteland M, Gauthier L, et al. The Firmicutes\/Bacteroidetes ratio: a relevant marker of gut dysbiosis in obese patients? Nutrients. 2020 May;12 (5):1474.","DOI":"10.3390\/nu12051474"},{"key":"40","doi-asserted-by":"crossref","unstructured":"40. Verheggen R, Konstanti P, Smidt H, Hermus A, Thijssen D, Hopman M. Eight-week exercise training in humans with obesity: Marked improvements in insulin sensitivity and modest changes in gut microbiome. Obesity (Silver Spring). 2021 Oct;29 (10):1615-24.","DOI":"10.1002\/oby.23252"},{"key":"41","doi-asserted-by":"crossref","unstructured":"41. Kang SS, Jeraldo PR, Kurti A, et al. Diet and exercise orthogonally alter the gut microbiome and reveal independent associations with anxiety and cognition. Mol Neurodegener. 2014 Sep;9:36.","DOI":"10.1186\/1750-1326-9-36"},{"key":"42","doi-asserted-by":"crossref","unstructured":"42. Sharma M, Li Y, Stoll ML, Tollefsbol TO. The epigenetic connection between the gut microbiome in obesity and diabetes. Front Genet [Internet]. 2020 Jan;10:1329. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/32010189","DOI":"10.3389\/fgene.2019.01329"},{"key":"43","doi-asserted-by":"crossref","unstructured":"43. Murri M, Leiva I, Gomez-Zumaquero JM, et al. Gut microbiota in children with type 1 diabetes differs from that in healthy children: a case-control study. BMC Medicine. 2013 Feb;11:46-58.","DOI":"10.1186\/1741-7015-11-46"},{"key":"44","doi-asserted-by":"crossref","unstructured":"44. Yoshimoto S, Loo TM, Atarashi K, et al. Obesity-induced gut microbial metabolite promotes liver cancer through senescence secretome. Nature [Internet]. 2013 Jul 4;499 (7456):97-101. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23803760","DOI":"10.1038\/nature12347"},{"key":"45","doi-asserted-by":"crossref","unstructured":"45. Gong S, Ye T, Wang M, et al. Traditional chinese medicine formula Kang Shuai Lao Pian improves obesity, gut dysbiosis, and fecal metabolic disorders in high-fat diet-fed mice. Frontiers in pharmacology [Internet]. 2020 Mar;11:297. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/32269525","DOI":"10.3389\/fphar.2020.00297"},{"key":"46","doi-asserted-by":"crossref","unstructured":"46. Wang Z, Zhao Y. Gut microbiota derived metabolites in cardiovascular health and disease. Protein Cell [Internet]. 2018 May;9 (5):416-31. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29725935","DOI":"10.1007\/s13238-018-0549-0"},{"key":"47","doi-asserted-by":"crossref","unstructured":"47. Versalovic J, Hemarajata P, Devaraj S. The human gut microbiome and body metabolism: Implications for obesity and diabetes. Clin Chem. 2013 Apr;59 (4):617-28.","DOI":"10.1373\/clinchem.2012.187617"},{"key":"48","doi-asserted-by":"crossref","unstructured":"48. Liu Z, Liu HY, Zhou H, et al. Moderate-intensity exercise affects gut microbiome composition and influences cardiac function in myocardial infarction mice. Front Microbiol [Internet]. 2017 Sep;8;1687. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28919891","DOI":"10.3389\/fmicb.2017.01687"},{"key":"49","doi-asserted-by":"crossref","unstructured":"49. Vazquez-Moreno M, Perez-Herrera A, Locia-Morales D, et al. Association of gut microbiome with fasting triglycerides, fasting insulin and obesity status in Mexican children. Pediatr Obes. 2021 May;16 (5):e12748.","DOI":"10.1111\/ijpo.12748"},{"key":"50","doi-asserted-by":"crossref","unstructured":"50. Therdtatha P, Song Y, Tanaka M, et al. Gut microbiome of indonesian adults associated with obesity and type 2 diabetes: A cross-sectional study in an asian city, Yogyakarta. Microorganisms [Internet]. 2021 Apr 22;9 (5):897. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/33922321","DOI":"10.3390\/microorganisms9050897"},{"key":"51","doi-asserted-by":"crossref","unstructured":"51. Wei Y, Liang J, Su Y, et al. The associations of the gut microbiome composition and short-chain fatty acid concentrations with body fat distribution in children. Clin Nutr (Edinburgh, Scotland). 2021 May;40 (5):3379-90.","DOI":"10.1016\/j.clnu.2020.11.014"},{"key":"52","doi-asserted-by":"crossref","unstructured":"52. Zheng B, Wang T, Wang H, Chen L, Zhou Z. Studies on nutritional intervention of rice starch-oleic acid complex (resistant starch type V) in rats fed by high-fat diet. Carbohydr Polym. 2020 Oct;246:116637.","DOI":"10.1016\/j.carbpol.2020.116637"},{"key":"53","doi-asserted-by":"crossref","unstructured":"53. Li B, He X, Lei SS, et al. Hypertensive rats treated chronically with N(omega)-Nitro-L-Arginine Methyl Ester (L-NAME) induced disorder of hepatic fatty acid metabolism and intestinal pathophysiology. Front Pharmacol [Internet]. 2019 Jan;10:1677. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/32076406","DOI":"10.3389\/fphar.2019.01677"},{"key":"54","doi-asserted-by":"crossref","unstructured":"54. Zhao X, Zhang Z, Hu B, Huang W, Yuan C, Zou L. Response of gut microbiota to metabolite changes induced by endurance exercise. Front Microbiol [Internet]. 2018 Apr;9:765. Available from: http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29731746","DOI":"10.3389\/fmicb.2018.00765"}],"container-title":["Journal of Nippon Medical School"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jnms\/89\/3\/89_JNMS.2022_89-307\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T05:44:09Z","timestamp":1715233449000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jnms\/89\/3\/89_JNMS.2022_89-307\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,25]]},"references-count":54,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.1272\/jnms.jnms.2022_89-307","relation":{},"ISSN":["1345-4676","1347-3409"],"issn-type":[{"value":"1345-4676","type":"print"},{"value":"1347-3409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,25]]},"article-number":"JNMS.2022_89-307"}}