{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T18:11:29Z","timestamp":1779905489849,"version":"3.53.1"},"reference-count":12,"publisher":"The Ewha Medical Journal, Ewha Womans University College of Medicine","issue":"2","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"content-domain":{"domain":["e-emj.org"],"crossmark-restriction":true},"short-container-title":["Ewha Med J"],"published-print":{"date-parts":[[2025,4,30]]},"abstract":"<jats:p>Purpose: This study aimed to investigate whether proteins present in the molting membranes of third-stage (L3) Anisakis larvae could serve as potential risk factors for allergic reactions.Methods: Third-stage larvae (L3) of Anisakis spp. were primarily collected from mackerels and cultured in vitro to yield both molting membranes and fourth-stage (L4) larvae. Major soluble proteins in the molting membranes were identified using SDS-PAGE (sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis). Crude antigens extracted from L3, L4, and the molting membranes were subsequently evaluated by western blotting using sera from Anisakis-infected rabbits and patients with eosinophilia.Results: Antigens derived from the molting membranes reacted with sera from Anisakis-infected rabbits as well as with sera from 7 patients with eosinophilia of unknown origin. These findings suggest that unidentified proteins in the molting membranes of Anisakis L3 may contribute to early allergic reactions, particularly in patients sensitized by specific molecular components.Conclusion: Our results indicate that proteins present in the molting membranes of third-stage Anisakis spp. larvae may be associated with allergic responses. Further studies are required to confirm the correlation between these membranes and Anisakis-induced allergies.<\/jats:p>","DOI":"10.12771\/emj.2025.00311","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T08:37:06Z","timestamp":1744101426000},"page":"e29","update-policy":"https:\/\/doi.org\/10.12771\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Immunogenicity of &lt;i&gt;Anisakis&lt;\/i&gt; larvae molting membrane against human eosinophilia sera"],"prefix":"10.12771","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8201-489X","authenticated-orcid":true,"given":"Sooji","family":"Hong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7581-3899","authenticated-orcid":true,"given":"Bong-Kwang","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3822-4002","authenticated-orcid":true,"given":"Hyun-Jong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4998","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-004-1085-9"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1021\/jf903492f"},{"key":"ref3","first-page":"97","volume-title":"A nematode parasitic to herring, causing acute abdominal syndromes in man","author":"van Thiel","year":"1960"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-017-0698-x"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2020.1749024"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2020.03.004"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3201\/eid1903.121017"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00012-07"},{"key":"ref9","first-page":"150","volume-title":"Anisakis simplex: current knowledge","author":"Pravettoni","year":"2012"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-020-06892-0"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.1991.29.4.389"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.2307\/3283849"}],"container-title":["Ewha Medical Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/e-emj.org\/upload\/pdf\/emj-2025-00311.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/e-emj.org\/journal\/view.php?doi=10.12771\/emj.2025.00311","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/e-emj.org\/upload\/pdf\/emj-2025-00311.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T07:43:34Z","timestamp":1745826214000},"score":1,"resource":{"primary":{"URL":"http:\/\/e-emj.org\/journal\/view.php?doi=10.12771\/emj.2025.00311"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,30]]},"references-count":12,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,4]]}},"URL":"https:\/\/doi.org\/10.12771\/emj.2025.00311","relation":{},"ISSN":["2234-3180","2234-2591"],"issn-type":[{"value":"2234-3180","type":"print"},{"value":"2234-2591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,30]]},"assertion":[{"value":"2025-03-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-08","order":2,"name":"published","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-30","order":3,"name":"published","label":"Print publication","group":{"name":"publication_history","label":"Publication History"}}]}}