{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T04:24:22Z","timestamp":1792211062976,"version":"4.4.0"},"reference-count":44,"publisher":"American Chemical Society (ACS)","issue":"4","license":[{"start":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T00:00:00Z","timestamp":1514505600000},"content-version":"vor","delay-in-days":4355,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Genistein, the major phytoestrogen in soy, is linked to diminished female reproductive performance and to cancer chemoprevention and decreased adipose deposition. Dietary genistein may also play a role in the decreased incidence of cancer in Asians compared with Westerners, as well as increased cancer incidence in Asians immigrating to the United States. Here, we report that maternal dietary genistein supplementation of mice during gestation, at levels comparable with humans consuming high-soy diets, shifted the coat color of heterozygous viable yellow agouti (Avy\/a) offspring toward pseudoagouti. This marked phenotypic change was significantly associated with increased methylation of six cytosine\u2013guanine sites in a retrotransposon upstream of the transcription start site of the Agouti gene. The extent of this DNA methylation was similar in endodermal, mesodermal, and ectodermal tissues, indicating that genistein acts during early embryonic development. Moreover, this genistein-induced hypermethylation persisted into adulthood, decreasing ectopic Agouti expression and protecting offspring from obesity. Thus, we provide the first evidence that in utero dietary genistein affects gene expression and alters susceptibility to obesity in adulthood by permanently altering the epigenome.<\/jats:p>","DOI":"10.1289\/ehp.8700","type":"journal-article","created":{"date-parts":[[2006,1,26]],"date-time":"2006-01-26T13:44:15Z","timestamp":1138283055000},"page":"567-572","source":"Crossref","is-referenced-by-count":819,"title":["Maternal Genistein Alters Coat Color and Protects\n                    <i>Avy<\/i>\n                    Mouse Offspring from Obesity by Modifying the Fetal Epigenome"],"prefix":"10.1021","volume":"114","author":[{"given":"Dana C.","family":"Dolinoy","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina, USA"},{"name":"University Program in Genetics and Genomics and"},{"name":"Integrated Toxicology Program, Duke University, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer R.","family":"Weidman","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina, USA"},{"name":"University Program in Genetics and Genomics and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Waterland","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and"},{"name":"Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randy L.","family":"Jirtle","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina, USA"},{"name":"University Program in Genetics and Genomics and"},{"name":"Integrated Toxicology Program, Duke University, Durham, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2006,1,26]]},"reference":[{"key":"2026080608091812900_micit1","doi-asserted-by":"crossref","first-page":"5592","DOI":"10.1016\/S0021-9258(18)45614-1","article-title":"Genistein, a specific inhibitor of tyrosine-specific protein kinases","volume":"262","author":"Akiyama","year":"1987","journal-title":"J Biol Chem"},{"key":"2026080608091812900_micit2","doi-asserted-by":"crossref","first-page":"559S","DOI":"10.1093\/jn\/132.3.559S","article-title":"The health consequences of early soy consumption","volume":"132","author":"Badger","year":"2002","journal-title":"J Nutr"},{"key":"2026080608091812900_micit3","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1111\/j.1651-2227.1997.tb18408.x","article-title":"Intrauterine programming of coronary heart disease and stroke","volume":"423","author":"Barker","year":"1997","journal-title":"Acta Paediatr Suppl"},{"issue":"6 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health","volume":"430","author":"Bateson","year":"2004","journal-title":"Nature"},{"key":"2026080608091812900_micit7","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1101\/gad.947102","article-title":"DNA methylation patterns and epigenetic memory","volume":"16","author":"Bird","year":"2002","journal-title":"Genes Dev"},{"key":"2026080608091812900_micit8","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1038\/labinvest.3780282","article-title":"Animal models impacted by phytoestrogens in commercial chow: implications for pathways influenced by hormones","volume":"81","author":"Brown","year":"2001","journal-title":"Lab Invest"},{"key":"2026080608091812900_micit9","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1667\/0033-7587(2001)155[0201:AHBECT]2.0.CO;2","article-title":"A human breast epithelial cell type with stem cell characteristics as target cells for carcinogenesis","volume":"155","author":"Chang","year":"2001","journal-title":"Radiat 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Perspectives"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/evhpaz\/article-pdf\/114\/4\/567\/66436208\/ehp.8700.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/evhpaz\/article-pdf\/114\/4\/567\/66436208\/ehp.8700.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T13:48:32Z","timestamp":1786628912000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/evhpaz\/article-lookup\/doi\/10.1289\/ehp.8700"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1,26]]},"references-count":44,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2006,1,26]]},"published-print":{"date-parts":[[2006,4,1]]}},"URL":"https:\/\/doi.org\/10.1289\/ehp.8700","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1011011.372723","asserted-by":"object"}]},"ISSN":["0091-6765","1552-9924"],"issn-type":[{"value":"0091-6765","type":"print"},{"value":"1552-9924","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,1,26]]}}}