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AbouLaila, M., Yokoyama, N. and Igarashi, I. 2010. Development and evaluation of two nested PCR assays for the detection of <i>Babesia bovis<\/i> from cattle blood. <i>Vet. Parasitol.<\/i> 172: 65\u201370.","DOI":"10.1016\/j.vetpar.2010.04.011"},{"key":"2","unstructured":"2. Almeria, S., Castella, J., Ferrer, D., Ortuno, A., Estrada-Pe\u00f1a, A. and Gutierrez, J. F. 2001. Bovine piroplasms in Minorca (Balearic Islands, Spain): a comparison of PCR-based and light microscopy detection. <i>Vet. Parasitol.<\/i> 99: 249\u2013259."},{"key":"3","unstructured":"3. Bai, Q., Liu, G., Zhang, L. and Zhou, J. 1990. Studies on the isolation and preservation a single species of bovine haematocytozoon: the finding and isolation of <i>Babesia ovata<\/i> in China. <i>Chin. J. Vet. Med<\/i> 16: 2\u20134."},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. B\u00f6se, R., Jorgensen, W. K., Dalgliesh, R. J., Friedhoff, K. T. and de Vos, A. J. 1995. 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S. and Buening, G. M. 1993. Multiplex polymerase chain reaction based assay for the detection of <i>Babesia bigemina, Babesia bovis<\/i> and <i>Anaplasma marginale<\/i> DNA in bovine blood. <i>Vet. Parasitol.<\/i> 50: 69\u201381."},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Fisher, T. G., McElwain, T. F. and Palmer, G. H. 2001. Molecular basis for variable expression of merozoite surface antigen gp45 among American isolates of <i>Babesia bigemina<\/i>. <i>Infect. Immun.<\/i> 69: 3782\u20133790.","DOI":"10.1128\/IAI.69.6.3782-3790.2001"},{"key":"10","unstructured":"10. Gayo, V., Romito, M., Nel, L. H., Solari, M. A. and Viljoen, G. J. 2003. PCR-based detection of the transovarial transmission of Uruguayan <i>Babesia bovis<\/i> and <i>Babesia bigemina<\/i> vaccine strains. <i>Onderstepoort J. Vet. Res.<\/i> 70: 197\u2013204."},{"key":"11","unstructured":"11. Hall, T. A. 1999. 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MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. <i>Brief. Bioinform.<\/i> 9: 299\u2013306.","DOI":"10.1093\/bib\/bbn017"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Machado, R. Z., Montassier, H. J., Pinto, A. A., Lemos, E. G., Machado, M. R. F., Valad\u00e3o, I. F. F., Barci, L. G. and Malheiros, E. B. 1997. An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against <i>Babesia bovis<\/i> in cattle. <i>Vet. Parasitol.<\/i> 71: 17\u201326.","DOI":"10.1016\/S0304-4017(97)00003-4"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16. Martins, T. M., Pedro, O. C., Caldeira, R. A., do Ros\u00e1rio, V. E., Neves, L. and Domingos, A. 2008. Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method. <i>Vet. Parasitol.<\/i> 153: 225\u2013230.","DOI":"10.1016\/j.vetpar.2008.01.037"},{"key":"17","unstructured":"17. McCosker, P. J. 1981. 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Res.<\/i> 71: 67\u201375.","DOI":"10.4102\/ojvr.v71i1.287"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21. Norval, R. A. I., Fivaz, B. H., Lawrence, J. A. and Daillecourt, T. 1983. Epidemiology of tick-borne diseases of cattle in Zimbabwe. I. Babesiosis. <i>Trop. Anim. Health Prod.<\/i> 15: 87\u201394.","DOI":"10.1007\/BF02239802"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22. Oliveira-Sequeira, T. C. G., Oliveira, M. C. S., Araujo, J. P.. and Amarante, A. F. T. 2005. PCR-based detection of <i>Babesia bovis<\/i> and <i>Babesia bigemina<\/i> in their natural host <i>Boophilus microplus<\/i> and cattle. <i>Int. J. Parasitol.<\/i> 35: 105\u2013111.","DOI":"10.1016\/j.ijpara.2004.09.002"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23. Ramos, C. A., Ara\u00fajo, F. R., Alves, L. C., de Souza, I. I., Guedes, D. S.. and Soares, C. O. 2012. 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Seroprevalence of <i>Babesia bovis<\/i> and <i>Babesia bigemina<\/i> in cattle in the Soutpansberg region, Limpopo Province, South Africa, associated with changes in vector-tick populations. <i>J. S. Afr. Vet. Assoc.<\/i> 77: 61\u201365."},{"key":"29","unstructured":"29. Werle, E., Schneider, C., Renner, M., V\u00f6lker, M. and Fiehn, W. 1994. Convenient single-step, one tube purification of PCR products for direct sequencing. <i>Nucleic Acids Res.<\/i> 22: 4354\u20134355."},{"key":"30","unstructured":"30. Yoshinari, T., Sivakumar, T., Asada, M., Battsetseg, B., Huang, X., Lan, D. T. B., Inpankaew, T., Ybanez, A., Alhassan, A., Thekisoe, O. M. M., de Macedo, A. C. C., Inokuma, H., Igarashi, I. and Yokoyama, N. 2013. A PCR based survey of <i>Babesia ovata<\/i> in cattle from various Asian, African and South American countries. <i>J. Vet. Med. 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