{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T22:29:33Z","timestamp":1778279373242,"version":"3.51.4"},"reference-count":44,"publisher":"Japanese Society of Veterinary Science","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Veterinary Medical Science","J. Vet. Med. Sci."],"published-print":{"date-parts":[[2017]]},"DOI":"10.1292\/jvms.17-0084","type":"journal-article","created":{"date-parts":[[2017,5,26]],"date-time":"2017-05-26T22:06:57Z","timestamp":1495836417000},"page":"1182-1190","source":"Crossref","is-referenced-by-count":15,"title":["Effects of pentosan polysulfate and polysulfated glycosaminoglycan on chondrogenesis of canine bone marrow-derived mesenchymal stem cells in alginate and micromass culture"],"prefix":"10.1292","volume":"79","author":[{"given":"Eugene C.","family":"BWALYA","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangho","family":"KIM","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"FANG","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. M. Suranji","family":"WIJEKOON","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"HOSOYA","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"OKUMURA","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"122","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1. Adesida, A. B., Mulet-Sierra, A. and Jomha, N. M. 2012. Hypoxia mediated isolation and expansion enhances the chondrogenic capacity of bone marrow mesenchymal stromal cells. <i>Stem Cell Res. Ther.<\/i> 3: 9.","DOI":"10.1186\/scrt100"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Aigner, T., Gebhard, P. M., Schmid, E., Bau, B., Harley, V. and P\u00f6schl, E. 2003. SOX9 expression does not correlate with type II collagen expression in adult articular chondrocytes. <i>Matrix Biol.<\/i> 22: 363\u2013372.","DOI":"10.1016\/S0945-053X(03)00049-0"},{"key":"3","unstructured":"3. Barbero, A., Grogan, S., Sch\u00e4fer, D., Heberer, M., Mainil-Varlet, P. and Martin, I. 2004. Age related changes in human articular chondrocyte yield, proliferation and post-expansion chondrogenic capacity. <i>Osteoarthritis Cartilage<\/i> 12: 476\u2013484."},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Bonaventure, J., Kadhom, N., Cohen-Solal, L., Ng, K. H., Bourguignon, J., Lasselin, C. and Freisinger, P. 1994. Reexpression of cartilage-specific genes by dedifferentiated human articular chondrocytes cultured in alginate beads. <i>Exp. Cell Res.<\/i> 212: 97\u2013104.","DOI":"10.1006\/excr.1994.1123"},{"key":"5","unstructured":"5. Bosnakovski, D., Mizuno, M., Kim, G., Takagi, S., Okumur, M. and Fujinag, T. 2006. Gene expression profile of bovine bone marrow mesenchymal stem cell during spontaneous chondrogenic differentiation in pellet culture system. <i>Jpn. J. Vet. Res.<\/i> 53: 127\u2013139."},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Bosnakovski, D., Mizuno, M., Kim, G., Ishiguro, T., Okumura, M., Iwanaga, T., Kadosawa, T. and Fujinaga, T. 2004. Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells in pellet cultural system. <i>Exp. Hematol.<\/i> 32: 502\u2013509.","DOI":"10.1016\/j.exphem.2004.02.009"},{"key":"7","unstructured":"7. Budsberg, S. C., Bergh, M. S., Reynolds, L. R. and Streppa, H. K. 2007. Evaluation of pentosan polysulfate sodium in the postoperative recovery from cranial cruciate injury in dogs: a randomized, placebo-controlled clinical trial. <i>Vet. Surg.<\/i> 36: 234\u2013244."},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. Coleman, R. M., Case, N. D. and Guldberg, R. E. 2007. Hydrogel effects on bone marrow stromal cell response to chondrogenic growth factors. <i>Biomaterials<\/i> 28: 2077\u20132086.","DOI":"10.1016\/j.biomaterials.2007.01.010"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Collier, S. and Ghosh, P. 1989. Evaluation of the effect of antiarthritic drugs on the secretion of proteoglycans by lapine chondrocytes using a novel assay procedure. <i>Ann. Rheum. Dis.<\/i> 48: 372\u2013381.","DOI":"10.1136\/ard.48.5.372"},{"key":"10","unstructured":"10. Costeseque, R., Emonds-Alt, X., Breliere, J. C. and Roncucci, R. 1986. Polysulphated polysaccharides: an in vitro study of their effects on proteoglycan biosynthesis by articular chondrocytes. <i>Arch. Int. Pharmacodyn. Ther.<\/i> 282: 196\u2013208."},{"key":"11","doi-asserted-by":"crossref","unstructured":"11. Darling, E. M. and Athanasiou, K. A. 2005. Rapid phenotypic changes in passaged articular chondrocyte subpopulations. <i>J. Orthop. Res.<\/i> 23: 425\u2013432.","DOI":"10.1016\/j.orthres.2004.08.008"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12. Freshney, I. R. 2010. Culture of animal cells; A manual of basic technique and specialized applications, 6th ed., Wiley-Blackwell, New Jersey.","DOI":"10.1002\/9780470649367"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. Fujiki, M., Shineha, J., Yamanokuchi, K., Misumi, K. and Sakamoto, H. 2007. Effects of treatment with polysulfated glycosaminoglycan on serum cartilage oligomeric matrix protein and C-reactive protein concentrations, serum matrix metalloproteinase-2 and -9 activities, and lameness in dogs with osteoarthritis. <i>Am. J. Vet. Res.<\/i> 68: 827\u2013833.","DOI":"10.2460\/ajvr.68.8.827"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. Ghosh, P. 1999. The pathobiology of osteoarthritis and the rationale for the use of pentosan polysulfate for its treatment. <i>Semin. Arthritis Rheum.<\/i> 28: 211\u2013267.","DOI":"10.1016\/S0049-0172(99)80021-3"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Ghosh, P., Wu, J., Shimmon, S., Zannettino, A. C., Gronthos, S. and Itescu, S. 2010. Pentosan polysulfate promotes proliferation and chondrogenic differentiation of adult human bone marrow-derived mesenchymal precursor cells. <i>Arthritis Res. Ther.<\/i> 12: R28.","DOI":"10.1186\/ar2935"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16. Glade, M. J. 1990. Polysulfated glycosaminoglycan accelerates net synthesis of collagen and glycosaminoglycans by arthritic equine cartilage tissues and chondrocytes. <i>Am. J. Vet. Res.<\/i> 51: 779\u2013785.","DOI":"10.2460\/ajvr.1990.51.05.779"},{"key":"17","unstructured":"17. Hamada, T., Sakai, T., Hiraiwa, H., Nakashima, M., Ono, Y., Mitsuyama, H. and Ishiguro, N. 2013. Surface markers and gene expression to characterize the differentiation of monolayer expanded human articular chondrocytes. <i>Nagoya J. Med. Sci.<\/i> 75: 101\u2013111."},{"key":"18","doi-asserted-by":"crossref","unstructured":"18. Hellio Le Graverand-Gastineau, M. P. 2009. OA clinical trials: current targets and trials for OA. Choosing molecular targets: what have we learned and where we are headed? <i>Osteoarthritis Cartilage<\/i> 17: 1393\u20131401.","DOI":"10.1016\/j.joca.2009.04.009"},{"key":"19","unstructured":"19. Ikeda, T., Kawaguchi, H., Kamekura, S., Ogata, N., Mori, Y., Nakamura, K., Ikegawa, S. and Chung, U. I. 2005. Distinct roles of Sox5, Sox6, and Sox9 in different stages of chondrogenic differentiation. <i>J. Bone Miner. Metab.<\/i> 23: 337\u2013340."},{"key":"20","doi-asserted-by":"crossref","unstructured":"20. Innes, J. F., Barr, A. R. and Sharif, M. 2000. Efficacy of oral calcium pentosan polysulphate for the treatment of osteoarthritis of the canine stifle joint secondary to cranial cruciate ligament deficiency. <i>Vet. Rec.<\/i> 146: 433\u2013437.","DOI":"10.1136\/vr.146.15.433"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21. Khan, W. S., Adesida, A. B. and Hardingham, T. E. 2007. Hypoxic conditions increase hypoxia-inducible transcription factor 2alpha and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients. <i>Arthritis Res. Ther.<\/i> 9: R55.","DOI":"10.1186\/ar2211"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22. Krasnokutsky, S., Attur, M., Palmer, G., Samuels, J. and Abramson, S. B. 2008. Current concepts in the pathogenesis of osteoarthritis. <i>Osteoarthritis Cartilage<\/i> 16Suppl 3: S1\u2013S3.","DOI":"10.1016\/j.joca.2008.06.025"},{"key":"23","unstructured":"23. Kyriacos, A. A., Darling, E. M., DuRaine, G. D., Hu, J. C. and Reddi, A. H. 2013 Articular cartilage. Taylor &amp; Francis Group, Florida."},{"key":"24","doi-asserted-by":"crossref","unstructured":"24. Lafont, J. E., Talma, S. and Murphy, C. L. 2007. Hypoxia-inducible factor 2alpha is essential for hypoxic induction of the human articular chondrocyte phenotype. <i>Arthritis Rheum.<\/i> 56: 3297\u20133306.","DOI":"10.1002\/art.22878"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25. Lafont, J. E., Talma, S., Hopfgarten, C. and Murphy, C. L. 2008. Hypoxia promotes the differentiated human articular chondrocyte phenotype through SOX9-dependent and -independent pathways. <i>J. Biol. Chem.<\/i> 283: 4778\u20134786.","DOI":"10.1074\/jbc.M707729200"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26. Lemare, F., Steimberg, N., Le Griel, C., Demignot, S. and Adolphe, M. 1998. Dedifferentiated chondrocytes cultured in alginate beads: restoration of the differentiated phenotype and of the metabolic responses to interleukin-1beta. <i>J. Cell. Physiol.<\/i> 176: 303\u2013313.","DOI":"10.1002\/(SICI)1097-4652(199808)176:2<303::AID-JCP8>3.0.CO;2-S"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27. Leung, V. Y. L., Gao, B., Leung, K. K. H., Melhado, I. G., Wynn, S. L., Au, T. Y. K., Dung, N. W. F., Lau, J. Y. B., Mak, A. C. Y., Chan, D. and Cheah, K. S. E. 2011. SOX9 governs differentiation stage-specific gene expression in growth plate chondrocytes via direct concomitant transactivation and repression. <i>PLoS Genet.<\/i> 7: e1002356.","DOI":"10.1371\/journal.pgen.1002356"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28. Majumdar, M. K., Wang, E. and Morris, E. A. 2001. BMP-2 and BMP-9 promotes chondrogenic differentiation of human multipotential mesenchymal cells and overcomes the inhibitory effect of IL-1. <i>J. Cell. Physiol.<\/i> 189: 275\u2013284.","DOI":"10.1002\/jcp.10025"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29. Mauck, R. L., Yuan, X. and Tuan, R. S. 2006. Chondrogenic differentiation and functional maturation of bovine mesenchymal stem cells in long-term agarose culture. <i>Osteoarthritis Cartilage<\/i> 14: 179\u2013189.","DOI":"10.1016\/j.joca.2005.09.002"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30. Munteanu, S. E., Ilic, M. Z. and Handley, C. J. 2000. Calcium pentosan polysulfate inhibits the catabolism of aggrecan in articular cartilage explant cultures. <i>Arthritis Rheum.<\/i> 43: 2211\u20132218.","DOI":"10.1002\/1529-0131(200010)43:10<2211::AID-ANR8>3.0.CO;2-D"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31. Mwale, F., Stachura, D., Roughley, P. and Antoniou, J. 2006. Limitations of using aggrecan and type X collagen as markers of chondrogenesis in mesenchymal stem cell differentiation. <i>J. Orthop. Res.<\/i> 24: 1791\u20131798.","DOI":"10.1002\/jor.20200"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32. N\u00f6th, U., Rackwitz, L., Heymer, A., Weber, M., Baumann, B., Steinert, A., Sch\u00fctze, N., Jakob, F. and Eulert, J. 2007. Chondrogenic differentiation of human mesenchymal stem cells in collagen type I hydrogels. <i>J. Biomed. Mater. Res. A<\/i> 83: 626\u2013635.","DOI":"10.1002\/jbm.a.31254"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33. Qvist, P., Bay-Jensen, A. C., Christiansen, C., Dam, E. B., Pastoureau, P. and Karsdal, M. A. 2008. The disease modifying osteoarthritis drug (DMOAD): Is it in the horizon? <i>Pharmacol. Res.<\/i> 58: 1\u20137.","DOI":"10.1016\/j.phrs.2008.06.001"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34. Rogachefsky, R. A., Dean, D. D., Howell, D. S. and Altman, R. D. 1993. Treatment of canine osteoarthritis with insulin-like growth factor-1 (IGF-1) and sodium pentosan polysulfate. <i>Osteoarthritis Cartilage<\/i> 1: 105\u2013114.","DOI":"10.1016\/S1063-4584(05)80025-1"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35. Sevalla, K., Todhunter, R. J., Vernier-Singer, M. and Budsberg, S. C. 2000. Effect of polysulfated glycosaminoglycan on DNA content and proteoglycan metabolism in normal and osteoarthritic canine articular cartilage explants. <i>Vet. Surg.<\/i> 29: 407\u2013414.","DOI":"10.1053\/jvet.2000.9139"},{"key":"36","doi-asserted-by":"crossref","unstructured":"36. Shen, M., Yoshida, E., Yan, W., Kawamoto, T., Suardita, K., Koyano, Y., Fujimoto, K., Noshiro, M. and Kato, Y. 2002. Basic helix-loop-helix protein DEC1 promotes chondrocyte differentiation at the early and terminal stages. <i>J. Biol. Chem.<\/i> 277: 50112\u201350120.","DOI":"10.1074\/jbc.M206771200"},{"key":"37","doi-asserted-by":"crossref","unstructured":"37. Singh, M., Pierpoint, M., Mikos, A. G. and Kasper, F. K. 2011. Chondrogenic differentiation of neonatal human dermal fibroblasts encapsulated in alginate beads with hydrostatic compression under hypoxic conditions in the presence of bone morphogenetic protein-2. <i>J. Biomed. Mater. Res. A<\/i> 98: 412\u2013424.","DOI":"10.1002\/jbm.a.33129"},{"key":"38","doi-asserted-by":"crossref","unstructured":"38. Soleimani, M. and Nadri, S. 2009. A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. <i>Nat. Protoc.<\/i> 4: 102\u2013106.","DOI":"10.1038\/nprot.2008.221"},{"key":"39","unstructured":"39. Studer, D., Millan, C., \u00d6zt\u00fcrk, E., Maniura-Weber, K. and Zenobi-Wong, M. 2012. Molecular and biophysical mechanisms regulating hypertrophic differentiation in chondrocytes and mesenchymal stem cells. <i>Eur. Cell. Mater.<\/i> 24: 118\u2013135, discussion 135."},{"key":"40","doi-asserted-by":"crossref","unstructured":"40. Sunaga, T., Oh, N., Hosoya, K., Takagi, S. and Okumura, M. 2012. Inhibitory effects of pentosan polysulfate sodium on MAP-kinase pathway and NF-\u03baB nuclear translocation in canine chondrocytes in vitro. <i>J. Vet. Med. Sci.<\/i> 74: 707\u2013711.","DOI":"10.1292\/jvms.11-0511"},{"key":"41","unstructured":"41. Takahashi, I., Nuckolls, G. H., Takahashi, K., Tanaka, O., Semba, I., Dashner, R., Shum, L. and Slavkin, H. C. 1998. Compressive force promotes sox9, type II collagen and aggrecan and inhibits IL-1beta expression resulting in chondrogenesis in mouse embryonic limb bud mesenchymal cells. <i>J. Cell Sci.<\/i> 111: 2067\u20132076."},{"key":"42","doi-asserted-by":"crossref","unstructured":"42. Tallheden, T., Bengtsson, C., Brantsing, C., Sj\u00f6gren-Jansson, E., Carlsson, L., Peterson, L., Brittberg, M. and Lindahl, A. 2005. Proliferation and differentiation potential of chondrocytes from osteoarthritic patients. <i>Arthritis Res. Ther.<\/i> 7: R560\u2013R568.","DOI":"10.1186\/ar1709"},{"key":"43","doi-asserted-by":"crossref","unstructured":"43. Todhunter, R. J. and Lust, G. 1994. Polysulfated glycosaminoglycan in the treatment of osteoarthritis. <i>J. Am. Vet. Med. Assoc.<\/i> 204: 1245\u20131251.","DOI":"10.2460\/javma.1994.204.08.1245"},{"key":"44","doi-asserted-by":"crossref","unstructured":"44. Vaughan-Scott, T. and Taylor, J. H. 1997. The pathophysiology and medical management of canine osteoarthritis. <i>J. S. Afr. Vet. Assoc.<\/i> 68: 21\u201325.","DOI":"10.4102\/jsava.v68i1.861"}],"container-title":["Journal of Veterinary Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jvms\/79\/7\/79_17-0084\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T11:05:22Z","timestamp":1719227122000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jvms\/79\/7\/79_17-0084\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017]]},"references-count":44,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017]]}},"URL":"https:\/\/doi.org\/10.1292\/jvms.17-0084","relation":{},"ISSN":["0916-7250","1347-7439"],"issn-type":[{"value":"0916-7250","type":"print"},{"value":"1347-7439","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017]]}}}