{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T13:37:01Z","timestamp":1778679421717,"version":"3.51.4"},"reference-count":37,"publisher":"Japanese Society of Veterinary Science","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Vet. Med. Sci.","The Journal of Veterinary Medical Science"],"published-print":{"date-parts":[[2019]]},"DOI":"10.1292\/jvms.19-0057","type":"journal-article","created":{"date-parts":[[2019,9,15]],"date-time":"2019-09-15T22:03:21Z","timestamp":1568585001000},"page":"1475-1484","source":"Crossref","is-referenced-by-count":10,"title":["Th1-biased immunoadjuvant effect of the recombinant B subunit of an            &lt;i&gt;Escherichia coli&lt;\/i&gt; heat-labile enterotoxin on an inactivated porcine          reproductive and respiratory syndrome virus antigen via intranasal immunization in          mice"],"prefix":"10.1292","volume":"81","author":[{"given":"Fei","family":"SU","sequence":"first","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Science,              Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310002,              China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihua","family":"XU","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Science,              Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310002,              China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"XUE","sequence":"additional","affiliation":[{"name":"Zhejiang Center of Animal Disease Control, Hangzhou,              Zhejiang 310020, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junxing","family":"LI","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Science,              Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310002,              China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"FU","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Science,              Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310002,              China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"YU","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Science,              Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310002,              China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sai","family":"WANG","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Science,              Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310002,              China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiufang","family":"YUAN","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Science,              Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310002,              China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"122","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1. Binjawadagi,                B.,              Dwivedi, V.,                Manickam,              C., Ouyang,                K., Wu,                Y., Lee,                L. J.,                Torrelles, J.              B. and Renukaradhya,                G. J.          2014. Adjuvanted poly(lactic-co-glycolic) acid            nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus            vaccine elicits cross-protective immune response in pigs.              <i>Int. J. Nanomedicine<\/i>          9: 679\u2013694.","DOI":"10.2147\/IJN.S56127"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Bouvet, J.                P., Decroix,                N. and                Pamonsinlapatham,              P.          2002. Stimulation of local antibody production: parenteral or            mucosal vaccination? <i>Trends Immunol.<\/i>          23: 209\u2013213.","DOI":"10.1016\/S1471-4906(02)02186-5"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3. Crawley, A.                M., Mallard,                B. and                Wilkie, B.              N.          2005. Genetic selection for high and low immune response in            pigs: effects on immunoglobulin isotype expression. <i>Vet.              Immunol. Immunopathol.<\/i>          108: 71\u201376.","DOI":"10.1016\/j.vetimm.2005.07.006"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Evans, A.                B., Loyd,                H.,                Dunkelberger, J.              R., van Tol,                S., Bolton,                M. J.,                Dorman, K.              S., Dekkers,                J. C. M. and                Carpenter,              S.          2017. Antigenic and Biological Characterization of ORF2-6            variants at early times following PRRSV Infection.              <i>Viruses<\/i>          9: 113.","DOI":"10.3390\/v9050113"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5. Harakuni,                T., Sugawa,                H., Komesu,                A., Tadano,                M. and                Arakawa,            T.          2005. Heteropentameric cholera toxin B subunit chimeric            molecules genetically fused to a vaccine antigen induce systemic and mucosal immune            responses: a potential new strategy to target recombinant vaccine antigens to mucosal            immune systems. <i>Infect. Immun.<\/i>          73: 5654\u20135665.","DOI":"10.1128\/IAI.73.9.5654-5665.2005"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Hu,                Y., Cong,                X., Chen,                L., Qi,                J., Wu,                X., Zhou,                M., Yoo,                D., Li,                F., Sun,                W., Wu,                J., Zhao,                X., Chen,                Z., Yu,                J., Du,                Y. and              Wang,            J.          2016. Synergy of TLR3 and 7 ligands significantly enhances            function of DCs to present inactivated PRRSV antigen through TRIF\/MyD88-NF-\u03baB signaling            pathway. <i>Sci. Rep.<\/i>          6: 23977.","DOI":"10.1038\/srep23977"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Kim,              H., Kim,                H. K.,              Jung, J. H.,                Choi, Y.            J., Kim,                J., Um,                C. G.,              Hyun, S. B.,                Shin, S.,                Lee, B.,                Jang, G.,                Kang, B.            K., Moon, H.                J. and Song,                D. S.          2011. The assessment of efficacy of porcine reproductive            respiratory syndrome virus inactivated vaccine based on the viral quantity and            inactivation methods. <i>Virol. J.<\/i>          8: 323.","DOI":"10.1186\/1743-422X-8-323"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. Kimura,              A. and                Kishimoto,              T.          2010. IL-6: regulator of Treg\/Th17 balance.              <i>Eur. J. Immunol.<\/i>          40: 1830\u20131835.","DOI":"10.1002\/eji.201040391"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Labarque,                G., Van                Gucht, S.,                Van Reeth,              K., Nauwynck,                H. and                Pensaert,              M.          2003. Respiratory tract protection upon challenge of pigs            vaccinated with attenuated porcine reproductive and respiratory syndrome virus            vaccines. <i>Vet. Microbiol.<\/i>          95: 187\u2013197.","DOI":"10.1016\/S0378-1135(03)00157-3"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Lee,              J., Kim,                Y. M., Kim,                J. H., Cho,                C. W.,              Jeon, J. W.,                Park, J.            K., Lee, S.                H., Jung,                B. G. and                Lee, B.            J.          2018. Nasal delivery of chitosan\/alginate nanoparticle            encapsulated bee (Apis mellifera) venom promotes antibody production and viral clearance            during porcine reproductive and respiratory syndrome virus infection by modulating T            cell related responses. <i>Vet. Immunol.              Immunopathol.<\/i>          200: 40\u201351.","DOI":"10.1016\/j.vetimm.2018.04.006"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11. Lee,              J., Yoo,                J. K.,              Sohn, H. J.,                Kang, H.            K., Kim,                D., Shin,                H. J. and                Kim, J.            H.          2015. Protective immunity against Naegleria fowleri infection            on mice immunized with the rNfa1 protein using mucosal adjuvants.              <i>Parasitol. Res.<\/i>          114: 1377\u20131385.","DOI":"10.1007\/s00436-015-4316-3"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12. Lei,              H., Peng,                X., Shu,                H. and              Zhao,            D.          2015. Intranasal immunization with live recombinant            Lactococcus lactis combined with heat-labile toxin B subunit protects chickens from            highly pathogenic avian influenza H5N1 virus. <i>J. Med.              Virol.<\/i>          87: 39\u201344.","DOI":"10.1002\/jmv.23983"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. Li,                Z., He,                Y., Xu,                X., Leng,                X., Li,                S., Wen,                Y., Wang,                F., Xia,                M., Cheng,                S. and Wu,                H.          2016. Pathological and immunological characteristics of            piglets infected experimentally with a HP-PRRSV TJ strain.              <i>BMC Vet. Res.<\/i>          12: 230.","DOI":"10.1186\/s12917-016-0854-x"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. Linhares, D.                C., Cano,                J. P.,                Wetzell,            T., Nerem,                J.,                Torremorell,              M. and Dee,                S. A.          2012. Effect of modified-live porcine reproductive and            respiratory syndrome virus (PRRSv) vaccine on the shedding of wild-type virus from an            infected population of growing pigs.            <i>Vaccine<\/i>          30: 407\u2013413.","DOI":"10.1016\/j.vaccine.2011.10.075"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Liu,              X., Dong,                Y., Wang,                J., Li,                L., Zhong,                Z., Li,                Y. P.,              Chen, S. J.,                Fu, Y. C.,                Xu, W. C.            and Wei, C.            J.          2017. VSV-G Viral Envelope Glycoprotein Prepared from              <i>Pichia pastoris<\/i> Enhances Transfection of DNA into Animal            Cells. <i>J. Microbiol. Biotechnol.<\/i>          27: 1098\u20131105.","DOI":"10.4014\/jmb.1611.11082"},{"key":"16","unstructured":"16. Ma,                X., Yao,                B., Zheng,                W. and Li,                L.          2010. Comparative study on characterization of recombinant B            subunit of E. coli heat-labile enterotoxin (rLTB) prepared from E. coli and P.            patoris. <i>J. Microbiol. Biotechnol.<\/i>          20: 550\u2013557."},{"key":"17","doi-asserted-by":"crossref","unstructured":"17. Ma,                Y.          2016. Recent advances in nontoxic Escherichia coli heat-labile            toxin and its derivative adjuvants. <i>Expert Rev.              Vaccines<\/i>          15: 1361\u20131371.","DOI":"10.1080\/14760584.2016.1182868"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18. Marchioro, S.                B., Fisch,                A., Gomes,                C. K.,                Jorge, S.,                Galli, V.,                Haesebrouck,              F., Maes,                D.,                Dellagostin,              O. and                Concei\u00e7\u00e3o, F.              R.          2014. Local and systemic immune responses induced by a            recombinant chimeric protein containing Mycoplasma hyopneumoniae antigens fused to the B            subunit of Escherichia coli heat-labile enterotoxin LTB.              <i>Vet. Microbiol.<\/i>          173: 166\u2013171.","DOI":"10.1016\/j.vetmic.2014.07.009"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19. Miyata,              T., Harakuni,                T., Taira,                T.,                Matsuzaki,              G. and                Arakawa,            T.          2012. Merozoite surface protein-1 of Plasmodium yoelii fused            via an oligosaccharide moiety of cholera toxin B subunit glycoprotein expressed in yeast            induced protective immunity against lethal malaria infection in mice.              <i>Vaccine<\/i>          30: 948\u2013958.","DOI":"10.1016\/j.vaccine.2011.11.059"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20. Miyata,              T., Harakuni,                T., Tsuboi,                T.,                Sattabongkot,              J., Kohama,                H.,                Tachibana,              M.,              Matsuzaki,            G., Torii,                M. and                Arakawa,            T.          2010. Plasmodium vivax ookinete surface protein Pvs25 linked            to cholera toxin B subunit induces potent transmission-blocking immunity by intranasal            as well as subcutaneous immunization. <i>Infect.              Immun.<\/i>          78: 3773\u20133782.","DOI":"10.1128\/IAI.00306-10"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21. Miyata,              T., Oshiro,                S.,                Harakuni,              T., Taira,                T.,                Matsuzaki,              G. and                Arakawa,            T.          2012. Physicochemically stable cholera toxin B subunit            pentamer created by peripheral molecular constraints imposed by de novo-introduced            intersubunit disulfide crosslinks.            <i>Vaccine<\/i>          30: 4225\u20134232.","DOI":"10.1016\/j.vaccine.2012.04.047"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22. Nagaputra, J.                C., Rollier,                C. S.,                Sadarangani,              M., Hoe,                J. C.,                Mehta, O.              H., Norheim,                G., Saleem,                M., Chan,                H.,              Derrick, J.              P., Feavers,                I.,              Pollard, A.              J. and Moxon,                E. R.          2014. Neisseria meningitidis native outer membrane vesicles            containing different lipopolysaccharide glycoforms as adjuvants for meningococcal and            nonmeningococcal antigens. <i>Clin. Vaccine              Immunol.<\/i>          21: 234\u2013242.","DOI":"10.1128\/CVI.00561-13"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23. Nan,              Y., Wu,                C., Gu,                G., Sun,                W., Zhang,                Y. J. and                Zhou, E.            M.          2017. Improved Vaccine against PRRSV: Current Progress and            Future Perspective. <i>Front. Microbiol.<\/i>          8: 1635.","DOI":"10.3389\/fmicb.2017.01635"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24. Newsted,                D.,              Fallahi, F.,                Golshani,              A. and Azizi,                A.          2015. Advances and challenges in mucosal adjuvant            technology. <i>Vaccine<\/i>          33: 2399\u20132405.","DOI":"10.1016\/j.vaccine.2015.03.096"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25. Nieuwenhuis,                N.,              Duinhof, T.              F. and van Nes,                A.          2012. Economic analysis of outbreaks of porcine reproductive            and respiratory syndrome virus in nine sow herds. <i>Vet.              Rec.<\/i>          170: 225.","DOI":"10.1136\/vr.100101"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26. Ouyang,              K., Hiremath,                J.,                Binjawadagi,              B., Shyu,                D. L.,                Dhakal, S.,                Arcos, J.,                Schleappi,              R., Holman,                L., Roof,                M.,                Torrelles, J.              B. and Renukaradhya,                G. J.          2016. Comparative analysis of routes of immunization of a live            porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in a heterologous            virus challenge study. <i>Vet. Res.            (Faisalabad)<\/i>          47: 45.","DOI":"10.1186\/s13567-016-0331-3"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27. Patel, G.                B., Ponce,                A., Zhou,                H. and              Chen,            W.          2008. Safety of intranasally administered archaeal lipid            mucosal vaccine adjuvant and delivery (AMVAD) vaccine in mice.              <i>Int. J. Toxicol.<\/i>          27: 329\u2013339.","DOI":"10.1080\/10915810802352703"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28. Piras,              F., Bollard,                S., Laval,                F., Joisel,                F.,              Reynaud, G.,                Charreyre,              C., Andreoni,                C. and                Juillard,              V.          2005. Porcine reproductive and respiratory syndrome (PRRS)            virus-specific interferon-gamma+ T-cell responses after PRRS virus infection or            vaccination with an inactivated PRRS vaccine. <i>Viral              Immunol.<\/i>          18: 381\u2013389.","DOI":"10.1089\/vim.2005.18.381"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29. Renukaradhya, G.                J., Meng,                X. J.,                Calvert, J.              G., Roof,                M. and                Lager, K.              M.          2015. Live porcine reproductive and respiratory syndrome virus            vaccines: Current status and future direction.              <i>Vaccine<\/i>          33: 4069\u20134080.","DOI":"10.1016\/j.vaccine.2015.06.092"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30. Renukaradhya, G.                J., Meng,                X. J.,                Calvert, J.              G., Roof,                M. and                Lager, K.              M.          2015. Inactivated and subunit vaccines against porcine            reproductive and respiratory syndrome: Current status and future            direction. <i>Vaccine<\/i>          33: 3065\u20133072.","DOI":"10.1016\/j.vaccine.2015.04.102"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31. Shi, K.              C., Guo,                X., Ge,                X. N., Liu,                Q. and              Yang, H.            C.          2010. Cytokine mRNA expression profiles in peripheral blood            mononuclear cells from piglets experimentally co-infected with porcine reproductive and            respiratory syndrome virus and porcine circovirus type 2.              <i>Vet. Microbiol.<\/i>          140: 155\u2013160.","DOI":"10.1016\/j.vetmic.2009.07.021"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32. Silva-Campa,                E.,                Flores-Mendoza,              L., Res\u00e9ndiz,                M.,                Pinelli-Saavedra,              A.,              Mata-Haro,            V., Mwangi,                W. and                Hern\u00e1ndez,              J.          2009. Induction of T helper 3 regulatory cells by dendritic            cells infected with porcine reproductive and respiratory syndrome virus.              <i>Virology<\/i>          387: 373\u2013379.","DOI":"10.1016\/j.virol.2009.02.033"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33. Su,                F., Patel,                G. B., Hu,                S. and              Chen,            W.          2016. Induction of mucosal immunity through systemic            immunization: Phantom or reality? <i>Hum. Vaccin.              Immunother.<\/i>          12: 1070\u20131079.","DOI":"10.1080\/21645515.2015.1114195"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34. Thanasarasakulpong,                A.,                Poolperm,              P., Tankaew,                P., Sawada,                T. and                Sthitmatee,              N.          2015. Protectivity conferred by immunization with intranasal            recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in            chickens. <i>J. Vet. Med. Sci.<\/i>          77: 321\u2013326.","DOI":"10.1292\/jvms.14-0532"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35. Wongyanin,                P.,                Buranapraditkun,              S.,                Chokeshai-Usaha,              K.,                Thanawonguwech,              R. and                Suradhat,              S.          2010. Induction of inducible CD4+CD25+Foxp3+ regulatory T            lymphocytes by porcine reproductive and respiratory syndrome virus            (PRRSV). <i>Vet. Immunol. Immunopathol.<\/i>          133: 170\u2013182.","DOI":"10.1016\/j.vetimm.2009.07.012"},{"key":"36","doi-asserted-by":"crossref","unstructured":"36. Zhang,              L., Zhou,                L., Ge,                X., Guo,                X., Han,                J. and              Yang,            H.          2016. The Chinese highly pathogenic porcine reproductive and            respiratory syndrome virus infection suppresses Th17 cells response in            vivo. <i>Vet. Microbiol.<\/i>          189: 75\u201385.","DOI":"10.1016\/j.vetmic.2016.05.001"},{"key":"37","unstructured":"37. Zhou,              X., Wang,                Y., Jiang,                P., Yuan,                X., Li,                J. and Du,                X.          2014. Dynamic and distribution of recombinant E.coli            heat-labile enterotoxin B subunit in duck. <i>Huabei              Nongxuebao<\/i>          29: 104\u2013109."}],"container-title":["Journal of Veterinary Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jvms\/81\/10\/81_19-0057\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,26]],"date-time":"2019-10-26T03:43:40Z","timestamp":1572061420000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jvms\/81\/10\/81_19-0057\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":37,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2019]]}},"URL":"https:\/\/doi.org\/10.1292\/jvms.19-0057","relation":{},"ISSN":["0916-7250","1347-7439"],"issn-type":[{"value":"0916-7250","type":"print"},{"value":"1347-7439","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019]]}}}