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Almstetter MF, Oefner PJ, Dettmer K.  2012. Comprehensive two-dimensional gas chromatography in metabolomics. <i>Anal Bioanal Chem<\/i> 402: 1993\u20132013.","DOI":"10.1007\/s00216-011-5630-y"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Alshehhi SA, Mohammed T, Zewude A, Bayissa B, Antony A, Degafa BA, Ameni G.  2024. Retrospective study on the cumulative incidence of milk fever in parturient cows: a case study in the United Arab Emirates. <i>BMC Vet Res<\/i> 20: 587.","DOI":"10.1186\/s12917-024-04455-4"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3. Altman DG, Royston P.  2006. The cost of dichotomising continuous variables. <i>BMJ<\/i> 332: 1080.","DOI":"10.1136\/bmj.332.7549.1080"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Benjamini Y, Hochberg Y.  1995. Controlling the false discovery rate: a practical and powerful approach to multiple testing. <i>J Royal Stat Soc Series B <\/i>(<i>Methodological<\/i>)<i>.<\/i> 57: 289\u2013300.","DOI":"10.1111\/j.2517-6161.1995.tb02031.x"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5. Benjamini Y, Yekutieli D.  2001. The control of the false discovery rate in multiple testing under dependency. <i>Ann Stat<\/i> 29: 1165\u20131188.","DOI":"10.1214\/aos\/1013699998"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Blaine J, Chonchol M, Levi M.  2015. Renal control of calcium, phosphate, and magnesium homeostasis. <i>Clin J Am Soc Nephrol<\/i> 10: 1257\u20131272.","DOI":"10.2215\/CJN.09750913"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Cai C, Kong Y, Wu D, Wang J.  2018. Changes of macrominerals and calcitropic hormones in serum of periparturient dairy cows subject to subclinical hypocalcaemia. <i>J Dairy Res<\/i> 85: 12\u201315.","DOI":"10.1017\/S0022029918000031"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. Cambiaghi A, Ferrario M, Masseroli M.  2017. Analysis of metabolomic data: tools, current strategies and future challenges for omics data integration. <i>Brief Bioinform<\/i> 18: 498\u2013510.","DOI":"10.1093\/bib\/bbw031"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Chen Y, Li EM, Xu LY.  2022. Guide to metabolomics analysis: a bioinformatics workflow. <i>Metabolites<\/i> 12: 357.","DOI":"10.3390\/metabo12040357"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Dunn WB, Broadhurst D, Begley P, Zelena E, Francis-McIntyre S, Anderson N, Brown M, Knowles JD, Halsall A, Haselden JN, Nicholls AW, Wilson ID, Kell DB, Goodacre R. Human Serum Metabolome (HUSERMET) Consortium. 2011. Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry. <i>Nat Protoc<\/i> 6: 1060\u20131083.","DOI":"10.1038\/nprot.2011.335"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11. Goff JP.  2008. The monitoring, prevention, and treatment of milk fever and subclinical hypocalcemia in dairy cows. <i>Vet J<\/i> 176: 50\u201357.","DOI":"10.1016\/j.tvjl.2007.12.020"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12. Goff JP, Liesegang A, Horst RL.  2014. Diet-induced pseudohypoparathyroidism: a hypocalcemia and milk fever risk factor. <i>J Dairy Sci<\/i> 97: 1520\u20131528.","DOI":"10.3168\/jds.2013-7467"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. Hansen SS, N\u00f8rgaard P, Pedersen C, J\u00f8rgensen RJ, Mellau LS, Enemark JD.  2003. The effect of subclinical hypocalcaemia induced by Na2EDTA on the feed intake and chewing activity of dairy cows. <i>Vet Res Commun<\/i> 27: 193\u2013205.","DOI":"10.1023\/A:1023340506782"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. Hauke J, Kossowski T.  2011. Comparison of values of Pearson\u2019s and Spearman\u2019s correlation coefficients on the same sets of data. <i>Quaest Geogr<\/i> 30: 87\u201393.","DOI":"10.2478\/v10117-011-0021-1"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Ichizawa S, Uenoyama R, Kawashima C, Miyazaki M, Yamagishi N, Miyazaki T.  2024. Serum metabolic profiling in pregnant Holstein cows 3 weeks prior to parturition using two-dimensional gas chromatography time-of-flight mass spectrometry. <i>J Vet Med Sci<\/i> 86: 606\u2013611.","DOI":"10.1292\/jvms.24-0091"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16. Liang D, Arnold LM, Stowe CJ, Harmon RJ, Bewley JM.  2017. Estimating US dairy clinical disease costs with a stochastic simulation model. <i>J Dairy Sci<\/i> 100: 1472\u20131486.","DOI":"10.3168\/jds.2016-11565"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17. Liu Q, Li C, Wanga V, Shepherd BE.  2018. Covariate-adjusted Spearman\u2019s rank correlation with probability-scale residuals. <i>Biometrics<\/i> 74: 595\u2013605.","DOI":"10.1111\/biom.12812"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18. Maldini G, Allen MS.  2018. Temporal effects of ruminal propionic acid infusion on feeding behavior of Holstein cows in the postpartum period. <i>J Dairy Sci<\/i> 101: 3077\u20133084.","DOI":"10.3168\/jds.2017-13857"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19. Mart\u00edn-Tereso J, Verstegen MW.  2011. A novel model to explain dietary factors affecting hypocalcaemia in dairy cattle. <i>Nutr Res Rev<\/i> 24: 228\u2013243.","DOI":"10.1017\/S0954422411000126"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20. Mekonnen SA, Alelgn Z, Saudik S, Molla W, Fentie T, Jemberu WT.  2022. Reduced milk production, economic losses, and risk factors associated to subclinical hypocalcemia in Holstein Friesian\u00d7Zebu crossbreed cows in North-West Ethiopia. <i>Front Vet Sci<\/i> 9: 771889.","DOI":"10.3389\/fvets.2022.771889"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21. Miyazaki T, Okada K, Yamashita T, Miyazaki M.  2017. Two-dimensional gas chromatography time-of-flight mass spectrometry-based serum metabolic fingerprints of neonatal calves before and after first colostrum ingestion. <i>J Dairy Sci<\/i> 100: 4354\u20134364.","DOI":"10.3168\/jds.2017-12557"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22. Mohler RE, Dombek KM, Hoggard JC, Young ET, Synovec RE.  2006. Comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry analysis of metabolites in fermenting and respiring yeast cells. <i>Anal Chem<\/i> 78: 2700\u20132709.","DOI":"10.1021\/ac052106o"},{"key":"23","unstructured":"23. Radostitis O, Gay C, Hinchcliff K, Contable PD.  2007. Veterinary Medicine: a Textbook of the Diseases of Cattle, Horses, Pigs, Sheep and Goat, WB Saunders, Oxford."},{"key":"24","doi-asserted-by":"crossref","unstructured":"24. Reinhardt TA, Lippolis JD, McCluskey BJ, Goff JP, Horst RL.  2011. Prevalence of subclinical hypocalcemia in dairy herds. <i>Vet J<\/i> 188: 122\u2013124.","DOI":"10.1016\/j.tvjl.2010.03.025"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25. Rodr\u00edguez EM, Ar\u00eds A, Bach A.  2017. Associations between subclinical hypocalcemia and postparturient diseases in dairy cows. <i>J Dairy Sci<\/i> 100: 7427\u20137434.","DOI":"10.3168\/jds.2016-12210"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26. Schober P, Boer C, Schwarte LA.  2018. Correlation coefficients: appropriate use and interpretation. <i>Anesth Analg<\/i> 126: 1763\u20131768.","DOI":"10.1213\/ANE.0000000000002864"},{"key":"27","unstructured":"27. Seifi HA, Kia S.  2017. Subclinical hypocalcemia in dairy cows: pathophysiology, consequences and monitoring. <i>Iran J Vet Sci Technol<\/i> 9: 2."},{"key":"28","doi-asserted-by":"crossref","unstructured":"28. Seifi HA, Leblanc SJ, Leslie KE, Duffield TF.  2011. Metabolic predictors of post-partum disease and culling risk in dairy cattle. <i>Vet J<\/i> 188: 216\u2013220.","DOI":"10.1016\/j.tvjl.2010.04.007"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29. Venjakob PL, Borchardt S, Heuwieser W.  2017. Hypocalcemia-cow-level prevalence and preventive strategies in German dairy herds. <i>J Dairy Sci<\/i> 100: 9258\u20139266.","DOI":"10.3168\/jds.2016-12494"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30. Weinert CH, Egert B, Kulling SE.  2015. On the applicability of comprehensive two-dimensional gas chromatography combined with a fast-scanning quadrupole mass spectrometer for untargeted large-scale metabolomics. <i>J Chromatogr A<\/i> 1405: 156\u2013167.","DOI":"10.1016\/j.chroma.2015.04.011"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31. Yamagishi N, Kawashima C.  2022. Prepartum measurement of serum biomarkers reflecting osteoclastic and osteoblastic bone metabolism for predicting the risk of milk fever in dairy cows. <i>J Dairy Res<\/i> 89: 1\u20139.","DOI":"10.1017\/S0022029922000218"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32. Yang Y, Liu C, Zhao W, Mazarji M, Ren L, Liu C, Pan J, Yan B.  2024. 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