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Sci.","The Journal of Veterinary Medical Science"],"published-print":{"date-parts":[[2026]]},"DOI":"10.1292\/jvms.26-0042","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T22:10:32Z","timestamp":1784153432000},"page":"1448-1455","source":"Crossref","is-referenced-by-count":0,"title":["Gut microbiome changes in a captive giant panda with cardiovascular          disease"],"prefix":"10.1292","volume":"88","author":[{"given":"Xueying","family":"WANG","sequence":"first","affiliation":[{"name":"Graduate School of Environment Science, Hokkaido University, Hokkaido,              Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoji","family":"UMEMOTO","sequence":"additional","affiliation":[{"name":"Kobe Oji Zoo, Hyogo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mizuki","family":"KATO","sequence":"additional","affiliation":[{"name":"Kobe Oji Zoo, Hyogo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"HAYAKAWA","sequence":"additional","affiliation":[{"name":"Faculty of Environment Earth Science, Hokkaido University, Hokkaido,              Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"122","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"1. Acquarone              M,                Salgado-Flores              A, Sundset              MA. 2020.            The bacterial microbiome in the small intestine of hooded seals              (<i>Cystophora cristata<\/i>).              <i>Microorganisms<\/i>          8: 1664.","DOI":"10.3390\/microorganisms8111664"},{"key":"2","unstructured":"2. Andrews              S. FastQC: a quality control          tool for high throughput sequence data. http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/ [accessed on May          10, 2026]."},{"key":"3","doi-asserted-by":"crossref","unstructured":"3. B\u00e4ckhed              F, Ley              RE,                Sonnenburg              JL, Peterson              DA, Gordon              JI. 2005.            Host-bacterial mutualism in the human intestine.              <i>Science<\/i>          307: 1915\u20131920.","DOI":"10.1126\/science.1104816"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Bolyen              E, Rideout              JR, Dillon              MR, Bokulich              NA, Abnet              CC,                Al-Ghalith              GA, Alexander              H, Alm              EJ, Arumugam              M, Asnicar              F, Bai              Y, Bisanz              JE, Bittinger              K, Brejnrod              A, Brislawn              CJ, Brown              CT, Callahan              BJ,                Caraballo-Rodr\u00edguez              AM, Chase              J, Cope              EK, Da Silva              R, Diener              C, Dorrestein              PC, Douglas              GM, Durall              DM, Duvallet              C, Edwardson              CF, Ernst              M, Estaki              M, Fouquier              J, Gauglitz              JM, Gibbons              SM, Gibson              DL, Gonzalez              A, Gorlick              K, Guo              J, Hillmann              B, Holmes              S, Holste              H,                Huttenhower              C, Huttley              GA, Janssen              S, Jarmusch              AK, Jiang              L, Kaehler              BD, Kang              KB, Keefe              CR, Keim              P, Kelley              ST, Knights              D, Koester              I, Kosciolek              T, Kreps              J, Langille              MGI, Lee              J, Ley              R, Liu              YX, Loftfield              E, Lozupone              C, Maher              M, Marotz              C, Martin              BD, McDonald              D, McIver              LJ, Melnik              AV, Metcalf              JL, Morgan              SC, Morton              JT, Naimey              AT,                Navas-Molina              JA, Nothias              LF, Orchanian              SB, Pearson              T, Peoples              SL, Petras              D, Preuss              ML, Pruesse              E, Rasmussen              LB, Rivers              A, Robeson              MS               2nd, Rosenthal              P, Segata              N, Shaffer              M, Shiffer              A, Sinha              R, Song              SJ, Spear              JR, Swafford              AD, Thompson              LR, Torres              PJ, Trinh              P, Tripathi              A, Turnbaugh              PJ, Ul-Hasan              S, van der                Hooft              JJJ, Vargas              F,                V\u00e1zquez-Baeza              Y, Vogtmann              E, von Hippel              M, Walters              W, Wan              Y, Wang              M, Warren              J, Weber              KC,                Williamson              CHD, Willis              AD, Xu              ZZ, Zaneveld              JR, Zhang              Y, Zhu              Q, Knight              R, Caporaso              JG. 2019.            Reproducible, interactive, scalable and extensible microbiome data            science using QIIME 2. <i>Nat Biotechnol<\/i>          37: 852\u2013857.","DOI":"10.7287\/peerj.preprints.27295v2"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5. Brice              KL, Trivedi              P, Jeffries              TC, Blyton              MDJ, Mitchell              C, Singh              BK, Moore              BD. 2019.            The Koala (<i>Phascolarctos cinereus<\/i>) faecal microbiome            differs with diet in a wild population.            <i>PeerJ<\/i>          7: e6534.","DOI":"10.7717\/peerj.6534"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6. Caspi              R, Altman              T, Billington              R, Dreher              K, Foerster              H, Fulcher              CA, Holland              TA, Keseler              IM, Kothari              A, Kubo              A,                Krummenacker              M,                Latendresse              M, Mueller              LA, Ong              Q, Paley              S, Subhraveti              P, Weaver              DS,                Weerasinghe              D, Zhang              P, Karp              PD. 2014.            The MetaCyc database of metabolic pathways and enzymes and the BioCyc            collection of Pathway\/Genome Databases. <i>Nucleic Acids              Res<\/i>          42: D459\u2013D471.","DOI":"10.1093\/nar\/gkt1103"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Chang              L, Zhang              D, Wang              Y, Ren              Z, Wu              Y, Zhang              Q, Zhao              G, Pan              G, Wang              X, Zhao              X, Tong              D. 2025.            Pathology of fatal Baylisascaris schroederi infection in a wild giant            panda. <i>Parasite<\/i>          32: 34.","DOI":"10.1051\/parasite\/2025026"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. Chen              HM, Chen              CC, Chen              CC, Wang              SC, Wang              CL, Huang              CH, Liou              JS, Liu              TW, Peng              HL, Lin              FM, Liu              CY, Weng              SL, Cheng              CJ, Hung              YF, Liao              CC, Huang              HD. 2018.            Gut microbiome changes in overweight male adults following bowel            preparation. <i>BMC Genomics<\/i>          19 Suppl 10: 904.","DOI":"10.1186\/s12864-018-5285-6"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Chilloux              J, Neves              AL, Boulang\u00e9              CL, Dumas              ME. 2016.            The microbial-mammalian metabolic axis: a critical symbiotic            relationship. <i>Curr Opin Clin Nutr Metab            Care<\/i>          19: 250\u2013256.","DOI":"10.1097\/MCO.0000000000000284"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Chow              J, Lee              SM, Shen              Y, Khosravi              A, Mazmanian              SK. 2010. Chapter          8\u2212host\u2013bacterial symbiosis in health and disease. pp. 243\u2013274. In: Advances in Immunology          (Sidonia F, Andrea C eds.), Academic Press, San Diego.","DOI":"10.1016\/B978-0-12-381300-8.00008-3"},{"key":"11","unstructured":"11. Csardi              G, Nepusz              T. 2006.            The igraph software package for complex network research.              <i>InterJournal<\/i>. <i>Complex            Syst<\/i>: 1695."},{"key":"12","unstructured":"12. Cs\u00e1rdi              G., Nepusz              T., Traag              V., Horv\u00e1t              S., Zanini              F., Noom              D. and M\u00fcller              K.          2025. igraph: Network Analysis and Visualization in R."},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. Davison              S., Mascellani                Bergo              A., Ward              Z., Sackett              A., Strykova              A., Jaimes              J. D., Travis              D., Clayton              J. B., Murphy              H. W.,                Danforth              M. D., Smith              B. K.,                Blekhman              R., Fuh              T., Niatou                Singa              F. S., Havlik              J.,                Petrzelkova              K. and Gomez              A.          2025. Cardiometabolic disease risk in gorillas is associated            with altered gut microbial metabolism. <i>npj Biofilms              Microbiomes<\/i>. 11:          1\u201315.","DOI":"10.1038\/s41522-025-00664-3"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14. DeSantis              TZ,                Hugenholtz              P, Larsen              N, Rojas              M, Brodie              EL, Keller              K, Huber              T, Dalevi              D, Hu              P, Andersen              GL. 2006.            Greengenes, a chimera-checked 16S rRNA gene database and workbench            compatible with ARB. <i>Appl Environ            Microbiol<\/i>          72: 5069\u20135072.","DOI":"10.1128\/AEM.03006-05"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Friedman              J, Alm              EJ. 2012.            Inferring correlation networks from genomic survey data.              <i>PLOS Comput Biol<\/i>          8: e1002687.","DOI":"10.1371\/journal.pcbi.1002687"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16. Guo              W, Mishra              S, Zhao              J, Tang              J, Zeng              B, Kong              F, Ning              R, Li              M, Zhang              H, Zeng              Y, Tian              Y, Zhong              Y, Luo              H, Liu              Y, Yang              J, Yang              M, Zhang              M, Li              Y, Ni              Q, Li              C, Wang              C, Li              D, Zhang              H, Zuo              Z, Li              Y. 2018.            Metagenomic study suggests that the gut microbiota of the Giant Panda              (<i>Ailuropoda melanoleuca<\/i>) may not be specialized for fiber            fermentation. <i>Front Microbiol<\/i>          9: 229.","DOI":"10.3389\/fmicb.2018.00229"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17. Hamada              K, Isobe              J, Hattori              K, Hosonuma              M, Baba              Y, Murayama              M, Narikawa              Y, Toyoda              H, Funayama              E, Tajima              K, Shida              M, Hirasawa              Y, Tsurui              T, Ariizumi              H, Ishiguro              T, Suzuki              R, Ohkuma              R, Kubota              Y, Sambe              T, Tsuji              M, Wada              S, Kiuchi              Y, Kobayashi              S, Kuramasu              A, Horiike              A, Kim              YG, Tsunoda              T, Yoshimura              K. 2023.              <i>Turicibacter<\/i> and <i>Acidaminococcus<\/i>            predict immune-related adverse events and efficacy of immune checkpoint            inhibitor. <i>Front Immunol<\/i>          14: 1164724.","DOI":"10.3389\/fimmu.2023.1164724"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18. Hayakawa              T, Sawada              A, Tanabe              AS, Fukuda              S, Kishida              T, Kurihara              Y, Matsushima              K, Liu              J,                Akomo-Okoue              EF, Gravena              W, Kashima              M, Suzuki              M, Kadowaki              K, Suzumura              T, Inoue              E, Sugiura              H, Hanya              G, Agata              K. 2018.            Improving the standards for gut microbiome analysis of fecal samples:            insights from the field biology of Japanese macaques on Yakushima          Island. <i>Primates<\/i>          59: 423\u2013436.","DOI":"10.1007\/s10329-018-0671-x"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19. Heberle              H, Meirelles              GV, da Silva              FR, Telles              GP, Minghim              R. 2015.            InteractiVenn: a web-based tool for the analysis of sets through Venn            diagrams. <i>BMC Bioinformatics<\/i>          16: 169.","DOI":"10.1186\/s12859-015-0611-3"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20. Ipek              V, Gocmen              H, Cangul              IT. 2017.            Isolation of streptococci from a fatal case of myocarditis in a captive            brown bear (ursus Arctos). <i>J Zoo Wildl            Med<\/i>          48: 269\u2013271.","DOI":"10.1638\/2015-0302.1"},{"key":"21","unstructured":"21.  IUCN Ailuropoda                melanoleuca:Swaisgood              R, Wang              D, Wei              F. The IUCN Red List of          Threatened Species 2016: e.T712A121745669. https:\/\/www.iucnredlist.org\/species\/712\/121745669 [accessed on May 10,          2026]."},{"key":"22","doi-asserted-by":"crossref","unstructured":"22. Ke\u0161nerov\u00e1              L, Emery              O, Troilo              M, Liberti              J, Erkosar              B, Engel              P. 2020.            Gut microbiota structure differs between honeybees in winter and            summer. <i>ISME J<\/i>          14: 801\u2013814.","DOI":"10.1038\/s41396-019-0568-8"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23. Krynak              KL, Burke              DJ, Martin              RA, Dennis              PM. 2017.            Gut microbiome composition is associated with cardiac disease in            zoo-housed western lowland gorillas (Gorilla gorilla gorilla).              <i>FEMS Microbiol Lett<\/i>          364: fnx149.","DOI":"10.1093\/femsle\/fnx149"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24. Li              J, Karim              MR, Li              J, Zhang              L, Zhang              L. 2020.            Review on parasites of wild and captive giant pandas (<i>Ailuropoda              melanoleuca<\/i>): Diversity, disease and conservation impact.              <i>Int J Parasitol Parasites Wildl<\/i>          13: 38\u201345.","DOI":"10.1016\/j.ijppaw.2020.07.007"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25. Liberti              J, Kay              T, Quinn              A, Kesner              L, Frank              ET, Cabirol              A, Richardson              TO, Engel              P, Keller              L. 2022.            The gut microbiota affects the social network of            honeybees. <i>Nat Ecol Evol<\/i>          6: 1471\u20131479.","DOI":"10.1038\/s41559-022-01840-w"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26. Lin              H, Peddada              SD. 2020.            Analysis of compositions of microbiomes with bias            correction. <i>Nat Commun<\/i>          11: 3514.","DOI":"10.1038\/s41467-020-17041-7"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27. Lin              H, Peddada              SD. 2024.            Multigroup analysis of compositions of microbiomes with covariate            adjustments and repeated measures. <i>Nat            Methods<\/i>          21: 83\u201391.","DOI":"10.1038\/s41592-023-02092-7"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28. Liu              A, Qiao              W, Ma              R, Yan              Q, Zhao              S, Lang              Y. 2025.            The detection of mixed infection with canine parvovirus, canine distemper            virus, and rotavirus in giant pandas by multiplex PCR.              <i>Vet Sci<\/i>          12: 81.","DOI":"10.3390\/vetsci12020081"},{"key":"29","doi-asserted-by":"crossref","unstructured":"29. McDonald              D, Jiang              Y, Balaban              M, Cantrell              K, Zhu              Q, Gonzalez              A, Morton              JT, Nicolaou              G, Parks              DH, Karst              SM, Albertsen              M, Hugenholtz              P, DeSantis              T, Song              SJ, Bartko              A, Havulinna              AS,                Jousilahti              P, Cheng              S, Inouye              M, Niiranen              T, Jain              M, Salomaa              V, Lahti              L, Mirarab              S, Knight              R. 2024.            Greengenes2 unifies microbial data in a single reference            tree. <i>Nat Biotechnol<\/i>          42: 715\u2013718.","DOI":"10.1038\/s41587-023-01845-1"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30. McDonald              D, Price              MN, Goodrich              J, Nawrocki              EP, DeSantis              TZ, Probst              A, Andersen              GL, Knight              R, Hugenholtz              P. 2012.            An improved Greengenes taxonomy with explicit ranks for ecological and            evolutionary analyses of bacteria and archaea. <i>ISME              J<\/i>          6: 610\u2013618.","DOI":"10.1038\/ismej.2011.139"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31. McKenney              EA, Maslanka              M, Rodrigo              A, Yoder              AD. 2018.            Bamboo specialists from two mammalian orders (Primates, Carnivora) share            a high number of low-abundance gut microbes. <i>Microb              Ecol<\/i>          76: 272\u2013284.","DOI":"10.1007\/s00248-017-1114-8"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32. McManamon              R. and                Lowenstine              L.          2012. Cardiovascular disease in great apes.              <i>Fowler\u2019s Zoo and Wild Animal medicine: Current            Therapy<\/i>. 7:          408\u2013415.","DOI":"10.1016\/B978-1-4377-1986-4.00053-6"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33. Osawa              R, Walsh              TP, Cork              SJ. 1993.            Metabolism of tannin-protein complex by facultatively anaerobic bacteria            isolated from koala feces.            <i>Biodegradation<\/i>          4: 91\u201399.","DOI":"10.1007\/BF00702325"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34. Shaffer              M, Thurimella              K, Sterrett              JD, Lozupone              CA. 2023.            SCNIC: Sparse correlation network investigation for compositional            data. <i>Mol Ecol Resour<\/i>          23: 312\u2013325.","DOI":"10.1111\/1755-0998.13704"},{"key":"35","unstructured":"35. Shiffman              ME, Soo              RM, Dennis              PG, Morrison              M, Tyson              GW,                Hugenholtz              P. 2017.            Gene and genome-centric analyses of koala and wombat fecal microbiomes            point to metabolic specialization for <i>Eucalyptus<\/i>            digestion. <i>PeerJ<\/i>          5: e4075."},{"key":"36","doi-asserted-by":"crossref","unstructured":"36. Tanabe              AS, Toju              H. 2013.            Two new computational methods for universal DNA barcoding: a benchmark            using barcode sequences of bacteria, archaea, animals, fungi, and land            plants. <i>PLoS One<\/i>          8: e76910.","DOI":"10.1371\/journal.pone.0076910"},{"key":"37","doi-asserted-by":"crossref","unstructured":"37. Vacca              M, Celano              G, Calabrese              FM,                Portincasa              P, Gobbetti              M, De Angelis              M. 2020.            The controversial role of human gut lachnospiraceae.              <i>Microorganisms<\/i>          8: 573.","DOI":"10.3390\/microorganisms8040573"},{"key":"38","doi-asserted-by":"crossref","unstructured":"38. Vastesaeger              MM, Delcourt              R. 1961.            Spontaneous atherosclerosis and diet in captive animals.              <i>Nutr Dieta Eur Rev Nutr Diet<\/i>          3: 174\u2013188.","DOI":"10.1159\/000174723"},{"key":"39","doi-asserted-by":"crossref","unstructured":"39. Wang              T, Xie              Y, Zheng              Y, Wang              C, Li              D, Koehler              AV, Gasser              RB. 2018.          Parasites of the Giant Panda: a risk factor in the conservation of a species. pp. 1\u201333.          In: Advances in Parasitology, Elsevier, San Diego.","DOI":"10.1016\/bs.apar.2017.12.003"},{"key":"40","unstructured":"40. Wang              Y, Xia              M, Li              X, Lu              Y, Zhao              S, Guo              X, She              R, Zhang              C, Zhao              Y, Pu              T. 2022.            Pathological diagnosis of parenchymatous myocarditis in Giant Panda.              <i>Ye Sheng Dong Wu Xue Bao. Yesheng            Dongwu<\/i>: 782\u2013787."},{"key":"41","doi-asserted-by":"crossref","unstructured":"41. Wei              F, Hu              Y, Yan              L, Nie              Y, Wu              Q, Zhang              Z. 2015.            Giant pandas are not an evolutionary cul-de-sac: evidence from            multidisciplinary research. <i>Mol Biol            Evol<\/i>          32: 4\u201312.","DOI":"10.1093\/molbev\/msu278"},{"key":"42","doi-asserted-by":"crossref","unstructured":"42. Wei              G, Lu              H, Zhou              Z, Xie              H, Wang              A, Nelson              K, Zhao              L. 2007.            The microbial community in the feces of the giant panda (Ailuropoda            melanoleuca) as determined by PCR-TGGE profiling and clone library            analysis. <i>Microb Ecol<\/i>          54: 194\u2013202.","DOI":"10.1007\/s00248-007-9225-2"},{"key":"43","unstructured":"43. World\u2019s captive panda population hits 757. https:\/\/english.news.cn\/20241126\/9bb1cd94e73b4386a4596a57da29bd6f\/c.html          [accessed on May 10, 2026]."},{"key":"44","doi-asserted-by":"crossref","unstructured":"44. Xue              Z, Zhang              W, Wang              L, Hou              R, Zhang              M, Fei              L, Zhang              X, Huang              H,                Bridgewater              LC, Jiang              Y, Jiang              C, Zhao              L, Pang              X, Zhang              Z. 2015.            The bamboo-eating giant panda harbors a carnivore-like gut microbiota,            with excessive seasonal variations.            <i>MBio<\/i>          6: e00022\u2013e15.","DOI":"10.1128\/mBio.00022-15"}],"container-title":["Journal of Veterinary Medical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jvms\/88\/9\/88_26-0042\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:32:53Z","timestamp":1790393573000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/jvms\/88\/9\/88_26-0042\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":44,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.1292\/jvms.26-0042","relation":{},"ISSN":["0916-7250","1347-7439"],"issn-type":[{"value":"0916-7250","type":"print"},{"value":"1347-7439","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]},"article-number":"26-0042"}}