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Petrof              BJ,                Shrager              JB,                Stedman              HH,                Kelly              AM, and                Sweeney              HL. Dystrophin            protects the sarcolemma from stresses developed during muscle            contraction. Proc Natl Acad Sci USA.            90: 3710\u20133714. 1993.","DOI":"10.1073\/pnas.90.8.3710"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3.  Kieny              P,                Chollet              S,                Delalande              P,                Le Fort              M,                Magot              A,                Pereon              Y, and                Perrouin Verbe              B. Evolution of            life expectancy of patients with Duchenne muscular dystrophy at AFM Yolaine de Kepper            centre between 1981 and 2011. Ann Phys Rehabil Med.            56: 443\u2013454. 2013.","DOI":"10.1016\/j.rehab.2013.06.002"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4.  Banks              GB, and                Chamberlain              JS. The value            of mammalian models for duchenne muscular dystrophy in developing therapeutic            strategies. Curr Top Dev Biol. 84:            431\u2013453. 2008.","DOI":"10.1016\/S0070-2153(08)00609-1"},{"key":"5","doi-asserted-by":"crossref","unstructured":"5.  Nakamura              A, and                Takeda              S. Mammalian            models of Duchenne Muscular Dystrophy: pathological characteristics and therapeutic            applications. J Biomed Biotechnol.            2011: 184393. 2011.","DOI":"10.1155\/2011\/184393"},{"key":"6","doi-asserted-by":"crossref","unstructured":"6.  Wells              DJ. Tracking            progress: an update on animal models for Duchenne muscular dystrophy.            Dis Model Mech. 11: dmm035774.            2018.","DOI":"10.1242\/dmm.035774"},{"key":"7","doi-asserted-by":"crossref","unstructured":"7.  Chu              V,                Otero              JM,                Lopez              O,                Sullivan              MF,                Morgan              JP,                Amende              I, and                Hampton              TG.            Electrocardiographic findings in mdx mice: a cardiac phenotype of            Duchenne muscular dystrophy. Muscle Nerve.            26: 513\u2013519. 2002.","DOI":"10.1002\/mus.10223"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8.  Branco              DM,                Wolf              CM,                Sherwood              M,                Hammer              PE,                Kang              PB, and                Berul              CI. Cardiac            electrophysiological characteristics of the mdx ( 5cv ) mouse model of Duchenne muscular            dystrophy. J Interv Card Electrophysiol.            20: 1\u20137. 2007.","DOI":"10.1007\/s10840-007-9168-z"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9.  Masubuchi              N,                Shidoh              Y,                Kondo              S,                Takatoh              J, and                Hanaoka              K. Subcellular            localization of dystrophin isoforms in cardiomyocytes and phenotypic analysis of            dystrophin-deficient mice reveal cardiac myopathy is predominantly caused by a            deficiency in full-length dystrophin. Exp Anim.            62: 211\u2013217. 2013.","DOI":"10.1538\/expanim.62.211"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10.  Nakamura              K,                Fujii              W,                Tsuboi              M,                Tanihata              J,                Teramoto              N,                Takeuchi              S,                Naito              K,                Yamanouchi              K, and                Nishihara              M. Generation            of muscular dystrophy model rats with a CRISPR\/Cas system. Sci            Rep. 4: 5635. 2014.","DOI":"10.1038\/srep05635"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11.  Chiang              DY,                Allen              HD,                Kim              JJ,                Valdes              SO,                Wang              Y,                Pignatelli              RH,                Lotze              TE, and                Miyake              CY. Relation of            cardiac dysfunction to rhythm abnormalities in patients with Duchenne or Becker muscular            dystrophies. Am J Cardiol. 117:            1349\u20131354. 2016.","DOI":"10.1016\/j.amjcard.2016.01.031"},{"key":"12","doi-asserted-by":"crossref","unstructured":"12.  Villa              CR,                Czosek              RJ,                Ahmed              H,                Khoury              PR,                Anderson              JB,                Knilans              TK,                Jefferies              JL,                Wong              B, and                Spar              DS. Ambulatory            monitoring and arrhythmic outcomes in pediatric and adolescent patients with Duchenne            muscular dystrophy. J Am Heart Assoc.            5: e002620. 2015.","DOI":"10.1161\/JAHA.115.002620"},{"key":"13","doi-asserted-by":"crossref","unstructured":"13.  Segawa              K,                Komaki              H,                Mori-Yoshimura              M,                Oya              Y,                Kimura              K,                Tachimori              H,                Kato              N,                Sasaki              M, and                Takahashi              Y. Cardiac            conduction disturbances and aging in patients with Duchenne muscular            dystrophy. Medicine (Baltimore). 96:            e8335. 2017.","DOI":"10.1097\/MD.0000000000008335"},{"key":"14","doi-asserted-by":"crossref","unstructured":"14.  Thomas              TO,                Morgan              TM,                Burnette              WB, and                Markham              LW. Correlation            of heart rate and cardiac dysfunction in Duchenne muscular dystrophy.            Pediatr Cardiol. 33:            1175\u20131179. 2012.","DOI":"10.1007\/s00246-012-0281-0"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15.  Nigro              G,                Comi              LI,                Politano              L, and                Bain              RJI. The            incidence and evolution of cardiomyopathy in Duchenne muscular          dystrophy. Int J Cardiol. 26:            271\u2013277. 1990.","DOI":"10.1016\/0167-5273(90)90082-G"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16.  Lanza              GA,                Dello Russo              A,                Giglio              V,                De Luca              L,                Messano              L,                Santini              C,                Ricci              E,                Damiani              A,                Fumagalli              G,                De Martino              G,                Mangiola              F, and                Bellocci              F. Impairment            of cardiac autonomic function in patients with Duchenne muscular dystrophy: relationship            to myocardial and respiratory function. Am Heart J.            141: 808\u2013812. 2001.","DOI":"10.1067\/mhj.2001.114804"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17.  Lisak              RP,                Truong Daniel              DT, and                Carroll              W. International Neurology: A          Clinical Approach. Wiley, New Jersey. 2009.","DOI":"10.1002\/9781444317008"},{"key":"18","unstructured":"18.  Sengupta              P. The            Laboratory Rat: Relating Its Age With Human\u2019s. Int J Prev            Med. 4: 624\u2013630.            2013."},{"key":"19","doi-asserted-by":"crossref","unstructured":"19.  Tochinai              R,                Nagata              Y,                Ando              M,                Hata              C,                Suzuki              T,                Asakawa              N,                Yoshizawa              K,                Uchida              K,                Kado              S,                Kobayashi              T,                Kaneko              K, and                Kuwahara              M.            Combretastatin A4 disodium phosphate-induced myocardial            injury. J Toxicol Pathol. 29:            163\u2013171. 2016.","DOI":"10.1293\/tox.2016-0012"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20.  Tochinai              R,                Komatsu              K,                Murakami              J,                Nagata              Y,                Ando              M,                Hata              C,                Suzuki              T,                Kado              S,                Kobayashi              T, and                Kuwahara              M.            Histopathological and functional changes in a single-dose model of            combretastatin A4 disodium phosphate-induced myocardial damage in rats.            J Toxicol Pathol. 31:            307\u2013313. 2018.","DOI":"10.1293\/tox.2018-0023"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21.  Kanda              Y.            Investigation of the freely available easy-to-use software \u2018EZR\u2019 for            medical statistics. Bone Marrow Transplant.            48: 452\u2013458. 2013.","DOI":"10.1038\/bmt.2012.244"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22.  Berridge              BR,                Mowat              V,                Nagai              H,                Nyska              A,                Okazaki              Y,                Clements              PJ,                Rinke              M,                Snyder              PW,                Boyle              MC, and                Wells              MY.            Non-proliferative and proliferative lesions of the cardiovascular system            of the rat and mouse. J Toxicol Pathol.            29(Suppl):            1S\u201347S. 2016.","DOI":"10.1293\/tox.29.3S-1"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23.  Larcher              T,                Lafoux              A,                Tesson              L,                Remy              S,                Thepenier              V,                Fran\u00e7ois              V,                Le Guiner              C,                Goubin              H,                Dutilleul              M,                Guigand              L,                Toumaniantz              G,                De Cian              A,                Boix              C,                Renaud              JB,                Cherel              Y,                Giovannangeli              C,                Concordet              JP,                Anegon              I, and                Huchet              C.            Characterization of dystrophin deficient rats: a new model for Duchenne            muscular dystrophy. PLoS One. 9:            e110371. 2014.","DOI":"10.1371\/journal.pone.0110371"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24.  Frankel              KA, and                Rosser              RJ. The            pathology of the heart in progressive muscular dystrophy: epimyocardial            fibrosis. Hum Pathol. 7:            375\u2013386. 1976.","DOI":"10.1016\/S0046-8177(76)80053-6"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25.  Nomura              H, and                Hizawa              K.            Histopathological study of the conduction system of the heart in Duchenne            progressive muscular dystrophy. Acta Pathol Jpn.            32: 1027\u20131033. 1982.","DOI":"10.1111\/j.1440-1827.1982.tb02082.x"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26.  Torres              LFB, and                Duchen              LW. The mutant            mdx: inherited myopathy in the mouse. Morphological studies of nerves, muscles and            end-plates. Brain. 110:            269\u2013299. 1987.","DOI":"10.1093\/brain\/110.2.269"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27.  Megeney              LA,                Kablar              B,                Perry              RLS,                Ying              C,                May              L, and                Rudnicki              MA. Severe            cardiomyopathy in mice lacking dystrophin and MyoD. Proc Natl            Acad Sci USA. 96: 220\u2013225.            1999.","DOI":"10.1073\/pnas.96.1.220"},{"key":"28","unstructured":"28.  FDA-Approved Drugs, from U.S. Food and Drug          Administration website, accessed May 28, 2020: https:\/\/www.accessdata.fda.gov\/scripts\/cder\/daf\/index.cfm?event=overview.process&amp;varApplNo=206488."},{"key":"29","unstructured":"29.  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Am J            Respir Crit Care Med. 170:            456\u2013465. 2004.","DOI":"10.1164\/rccm.200307-885ST"}],"container-title":["Journal of Toxicologic Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/tox\/33\/4\/33_2020-0018\/_pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T03:44:05Z","timestamp":1604115845000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jstage.jst.go.jp\/article\/tox\/33\/4\/33_2020-0018\/_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020]]}},"URL":"https:\/\/doi.org\/10.1293\/tox.2020-0018","relation":{},"ISSN":["0914-9198","1881-915X"],"issn-type":[{"value":"0914-9198","type":"print"},{"value":"1881-915X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020]]}}}