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Stern              T, Davis              AM. 2016.            Evaluation and treatment of patients with constipation.            JAMA 315: 192\u2013193.","DOI":"10.1001\/jama.2015.16995"},{"key":"2","doi-asserted-by":"crossref","unstructured":"2. Xu              J, Zhou              X, Chen              C, Deng              Q, Huang              Q, Yang              J, Yang              N, Huang              F. 2012.            Laxative effects of partially defatted flaxseed meal on normal and            experimental constipated mice. BMC Complement Altern            Med 12: 14.","DOI":"10.1186\/1472-6882-12-14"},{"key":"3","doi-asserted-by":"crossref","unstructured":"3. Collins              MD, Gibson              GR. 1999.            Probiotics, prebiotics, and synbiotics: approaches for modulating the            microbial ecology of the gut. Am J Clin Nutr 69: 1052S\u20131057S.","DOI":"10.1093\/ajcn\/69.5.1052s"},{"key":"4","doi-asserted-by":"crossref","unstructured":"4. Naseer              M, Poola              S, Uraz              S, Tahan              V. 2020.            Therapeutic effects of prebiotics on constipation: a schematic            review. Curr Clin Pharmacol 15:            207\u2013215.","DOI":"10.2174\/1574884715666200212125035"},{"key":"5","unstructured":"5. Adachi              T. 1982. A study          on productions and applications of fructosyltransferase by <i>Aureobasidium            pullulans<\/i> var. <i>melarigenum<\/i>. The University of Tokyo (Academic          dissertation) 1\u2013188."},{"key":"6","unstructured":"6. Adachi              T. 1983.            Neosugar as a new health food ingredient. J Food            Sci 22: 71\u201378."},{"key":"7","doi-asserted-by":"crossref","unstructured":"7. Endo              H, Tamura              K, Fukasawa              T, Kanegae              M, Koga              J. 2012.            Comparison of fructooligosaccharide utilization by            <i>Lactobacillus<\/i> and              <i>Bacteroides<\/i> species. Biosci Biotechnol            Biochem 76: 176\u2013179.","DOI":"10.1271\/bbb.110496"},{"key":"8","doi-asserted-by":"crossref","unstructured":"8. Gibson              GR,                Roberfroid              MB. 1995.            Dietary modulation of the human colonic microbiota: introducing the            concept of prebiotics. J Nutr 125:            1401\u20131412.","DOI":"10.1093\/jn\/125.6.1401"},{"key":"9","doi-asserted-by":"crossref","unstructured":"9. Kolida              S, Tuohy              K, Gibson              GR. 2002.            Prebiotic effects of inulin and oligofructose. Br            J Nutr 87 Suppl 2:            S193\u2013S197.","DOI":"10.1079\/BJN\/2002537"},{"key":"10","doi-asserted-by":"crossref","unstructured":"10. Sako              T, Matsumoto              K, Tanaka              R. 1999.            Recent progress on research and applications of non-digestible            galacto-oligosaccharides. Int Dairy J 9: 69\u201380.","DOI":"10.1016\/S0958-6946(99)00046-1"},{"key":"11","doi-asserted-by":"crossref","unstructured":"11. Sannohe              Y, Fukasawa              T, Koga              J, Kubota              H, Kanegae              M. 2008.            Comparison of the growth of bifidobacteria in two culture media            containing either 1-kestose (GF<sub>2<\/sub>) or nystose          (GF<sub>3<\/sub>). Biosci Microflora 27:            13\u201317.","DOI":"10.12938\/bifidus.27.13"},{"key":"12","unstructured":"12. Macfarlane              GT, Steed              H, Macfarlane              S. 2008.            Bacterial metabolism and health-related effects of            galacto-oligosaccharides and other prebiotics. J Appl            Microbiol 104: 305\u2013344."},{"key":"13","doi-asserted-by":"crossref","unstructured":"13. Liu              J, Huang              XE. 2014.            Efficacy of <i>Bifidobacterium tetragenous<\/i> viable bacteria            tablets for cancer patients with functional constipation. Asian            Pac J Cancer Prev 15:            10241\u201310244.","DOI":"10.7314\/APJCP.2014.15.23.10241"},{"key":"14","doi-asserted-by":"publisher","unstructured":"14. Hatano              T, Miyatake              H, Natsume              M, Osakabe              N, Takizawa              T, Ito              H, Yoshida              T. 2002.            Proanthocyanidin glycosides and related polyphenols from cacao liquor and            their antioxidant effects. Phytochemistry 59: 749\u2013758.","DOI":"10.1016\/S0031-9422(02)00051-1"},{"key":"15","doi-asserted-by":"crossref","unstructured":"15. Natsume              M, Baba              S. 2014.            Suppressive effects of cacao polyphenols on the development of            atherosclerosis in apolipoprotein E-deficient mice. Subcell            Biochem 77: 189\u2013198.","DOI":"10.1007\/978-94-007-7920-4_16"},{"key":"16","doi-asserted-by":"crossref","unstructured":"16. Sanbongi              C, Osakabe              N, Natsume              M, Takizawa              T, Gomi              S, Osawa              T. 1998.            A ntioxidative polyphenols isolated from <i>Theobroma            cacao<\/i>. J Agric Food Chem 46:            454\u2013457.","DOI":"10.1021\/jf970575o"},{"key":"17","doi-asserted-by":"crossref","unstructured":"17. Sannohe              Y, Gomi              S, Murata              T, Ohyama              M, Yonekura              K, Kanegae              M, Koga              J. 2011.            A new glycosylated dihydrophaseic acid from cacao germs              (<i>Theobroma cacao<\/i> L.). Biosci Biotechnol            Biochem 75: 1606\u20131607.","DOI":"10.1271\/bbb.110140"},{"key":"18","doi-asserted-by":"crossref","unstructured":"18. Selmi              C, Mao              TK, Keen              CL, Schmitz              HH, Eric                Gershwin              M. 2006.            The anti-inflammatory properties of cocoa flavanols.            J Cardiovasc Pharmacol 47 Suppl              2: S163\u2013S171, discussion S172\u2013S176.","DOI":"10.1097\/00005344-200606001-00010"},{"key":"19","doi-asserted-by":"crossref","unstructured":"19. Shrime              MG, Bauer              SR, McDonald              AC, Chowdhury              NH, Coltart              CE, Ding              EL. 2011.            Flavonoid-rich cocoa consumption affects multiple cardiovascular risk            factors in a meta-analysis of short-term studies. J            Nutr 141: 1982\u20131988.","DOI":"10.3945\/jn.111.145482"},{"key":"20","doi-asserted-by":"crossref","unstructured":"20. Yamagishi              M, Natsume              M, Osakabe              N, Okazaki              K, Furukawa              F, Imazawa              T, Nishikawa              A, Hirose              M. 2003.            Chemoprevention of lung carcinogenesis by cacao liquor proanthocyanidins            in a male rat multi-organ carcinogenesis model. Cancer            Lett 191: 49\u201357.","DOI":"10.1016\/S0304-3835(02)00629-8"},{"key":"21","doi-asserted-by":"crossref","unstructured":"21. Yasuda              A, Natsume              M, Osakabe              N, Kawahata              K, Koga              J. 2011.            Cacao polyphenols influence the regulation of apolipoprotein in HepG2 and            Caco2 cells. J Agric Food Chem 59:            1470\u20131476.","DOI":"10.1021\/jf103820b"},{"key":"22","doi-asserted-by":"crossref","unstructured":"22. Koga              J, Ojiro              K, Yanagida              A, Suto              T, Hiki              H, Inoue              Y, Sakai              C, Nakamoto              K, Fujisawa              Y, Orihara              A, et            al. 2022. Ingestion of indigestible            cacao proteins promotes defecation and alters the intestinal microbiota in            mice. Curr Dev Nutr 6:            nzac129.","DOI":"10.1093\/cdn\/nzac129"},{"key":"23","doi-asserted-by":"crossref","unstructured":"23. Castillejo              G, Bull\u00f3              M, Anguera              A, Escribano              J,                Salas-Salvad\u00f3              J. 2006.            A controlled, randomized, double-blind trial to evaluate the effect of a            supplement of cocoa husk that is rich in dietary fiber on colonic transit in constipated            pediatric patients. Pediatrics 118:            e641\u2013e648.","DOI":"10.1542\/peds.2006-0090"},{"key":"24","doi-asserted-by":"crossref","unstructured":"24. Jenkins              DJ, Kendall              CW, Vuksan              V, Vidgen              E, Wong              E, Augustin              LS, Fulgoni              V              3rd. 2000.            Effect of cocoa bran on low-density lipoprotein oxidation and fecal            bulking. Arch Intern Med 160:            2374\u20132379.","DOI":"10.1001\/archinte.160.15.2374"},{"key":"25","doi-asserted-by":"crossref","unstructured":"25. Digesu              GA, Panayi              D, Kundi              N, Tekkis              P, Fernando              R, Khullar              V. 2010.            Validity of the Rome III Criteria in assessing constipation in            women. Int Urogynecol J 21:            1185\u20131193.","DOI":"10.1007\/s00192-010-1179-0"},{"key":"26","doi-asserted-by":"crossref","unstructured":"26. Ogata              T, Nakanura              T, Anjitsu              K, Yaeshima              T, Takahashi              S, Fukuwatari              Y, Ishibashi              N, Hayasawa              H, Fujisawa              T, Iino              H. 1997.            Effect of <i>Bifidobacterium longum<\/i> BB536 administration on            the intestinal environment, defecation frequency and fecal characteristics of human            volunteers. Biosci Microflora 16:            53\u201358.","DOI":"10.12938\/bifidus1996.16.53"},{"key":"27","doi-asserted-by":"crossref","unstructured":"27. Takahashi              S, Tomita              J, Nishioka              K, Hisada              T, Nishijima              M. 2014.            Development of a prokaryotic universal primer for simultaneous analysis            of bacteria and archaea using next-generation sequencing. PLoS            One 9: e105592.","DOI":"10.1371\/journal.pone.0105592"},{"key":"28","doi-asserted-by":"crossref","unstructured":"28. Caporaso              JG, Lauber              CL, Walters              WA,                Berg-Lyons              D, Huntley              J, Fierer              N, Owens              SM, Betley              J, Fraser              L, Bauer              M, et            al. 2012. Ultra-high-throughput            microbial community analysis on the Illumina HiSeq and MiSeq platforms.            ISME J 6: 1621\u20131624.","DOI":"10.1038\/ismej.2012.8"},{"key":"29","doi-asserted-by":"publisher","unstructured":"29. Schmieder              R, Edwards              R. 2011.            Quality control and preprocessing of metagenomic          datasets. Bioinformatics 27:            863\u2013864.","DOI":"10.1093\/bioinformatics\/btr026"},{"key":"30","doi-asserted-by":"crossref","unstructured":"30. Kleessen              B, Sykura              B, Zunft              HJ, Blaut              M. 1997.            Effects of inulin and lactose on fecal microflora, microbial activity,            and bowel habit in elderly constipated persons. Am J Clin            Nutr 65: 1397\u20131402.","DOI":"10.1093\/ajcn\/65.5.1397"},{"key":"31","doi-asserted-by":"crossref","unstructured":"31. Tramonte              SM, Brand              MB, Mulrow              CD, Amato              MG, O\u2019Keefe              ME, Ramirez              G. 1997.            The treatment of chronic constipation in adults. A systematic            review. J Gen Intern Med 12:            15\u201324.","DOI":"10.1046\/j.1525-1497.1997.12103.x"},{"key":"32","doi-asserted-by":"crossref","unstructured":"32. Miquel              S, Mart\u00edn              R, Rossi              O,                Berm\u00fadez-Humar\u00e1n              LG, Chatel              JM, Sokol              H, Thomas              M, Wells              JM, Langella              P. 2013.          <i>Faecalibacterium prausnitzii<\/i> and human            intestinal health. Curr Opin Microbiol 16: 255\u2013261.","DOI":"10.1016\/j.mib.2013.06.003"},{"key":"33","doi-asserted-by":"crossref","unstructured":"33. Yan              Z, Zhang              M, Xu              M, Yu              J, Copeland              L, Huang              Y, Wang              S. 2022.            Effect of debranching and differential ethanol precipitation on the            formation and fermentation properties of maize starch-lipid complexes.            J Agric Food Chem 70:            9132\u20139142.","DOI":"10.1021\/acs.jafc.2c03081"},{"key":"34","doi-asserted-by":"crossref","unstructured":"34. Duncan              SH, Aminov              RI, Scott              KP, Louis              P, Stanton              TB, Flint              HJ. 2006.            Proposal of <i>Roseburia faecis<\/i> sp. nov., <i>Roseburia              hominis<\/i> sp. nov. and <i>Roseburia inulinivorans<\/i> sp. nov., based            on isolates from human faeces. Int J Syst Evol            Microbiol 56: 2437\u20132441.","DOI":"10.1099\/ijs.0.64098-0"},{"key":"35","doi-asserted-by":"crossref","unstructured":"35. Grider              JR, Piland              BE. 2007.            The peristaltic reflex induced by short-chain fatty acids is mediated by            sequential release of 5-HT and neuronal CGRP but not BDNF. Am J            Physiol Gastrointest Liver Physiol 292:            G429\u2013G437.","DOI":"10.1152\/ajpgi.00376.2006"},{"key":"36","doi-asserted-by":"crossref","unstructured":"36. Sato              T, Matsumoto              K, Okumura              T, Yokoi              W, Naito              E, Yoshida              Y, Nomoto              K, Ito              M, Sawada              H. 2008.            Isolation of lactate-utilizing butyrate-producing bacteria from human            feces and <i>in vivo<\/i> administration of            <i>Anaerostipes caccae<\/i>            strain L2 and galacto-oligosaccharides in a rat model. FEMS            Microbiol Ecol 66: 528\u2013536.","DOI":"10.1111\/j.1574-6941.2008.00528.x"},{"key":"37","doi-asserted-by":"crossref","unstructured":"37. Geirnaert              A, Steyaert              A, Eeckhaut              V, Debruyne              B, Arends              JB, Van                Immerseel              F, Boon              N, Van de                Wiele              T. 2014.              <i>Butyricicoccus pullicaecorum<\/i>, a butyrate producer with            probiotic potential, is intrinsically tolerant to stomach and small intestine            conditions. Anaerobe 30:            70\u201374.","DOI":"10.1016\/j.anaerobe.2014.08.010"},{"key":"38","doi-asserted-by":"crossref","unstructured":"38. Hajjar              R, Richard              CS, Santos              MM. 2021.            The role of butyrate in surgical and oncological outcomes in colorectal            cancer. 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