{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T23:35:24Z","timestamp":1715556924172},"reference-count":0,"publisher":"Georg Thieme Verlag KG","issue":"05","license":[{"start":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T00:00:00Z","timestamp":1537747200000},"content-version":"vor","delay-in-days":1089,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aorta"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:p>\n            \n              Background:\n             Since aortic diameter is the most \u00adsignificant risk factor for rupture, we sought to identify stress-dependent changes in gene expression to illuminate novel molecular processes in aneurysm rupture.<\/jats:p><jats:p>\n            \n              Materials and Methods:\n             We constructed finite element maps of abdominal computerized tomography scans (CTs) of seven abdominal aortic aneurysm (AAA) patients to map wall stress. Paired biopsies from high- and low-stress areas were collected at surgery using vascular landmarks as coordinates. Differential gene expression was evaluated by Illumina Array analysis, using the whole genome DNA-mediated, annealing, selection, extension, and ligation (DASL) gene chip (n = 3 paired samples).<\/jats:p><jats:p>\n            \n              Results:\n             The sole significant candidate from this analysis, Lamin A\/C, was validated at the protein level, using western blotting. Lamin A\/C expression in the inferior mesenteric vein (IMV) of AAA patients was compared to a control group and in aortic smooth muscle cells in culture in response to physiological pulsatile stretch. \u00adAreas of high wall stress (n = 7) correlate to those \u00adregions which have the thinnest walls [778 \u00b5m (585\u20131120 \u00b5m)] in comparison to areas of lowest wall stress [1620 \u00b5m (962\u20132919 \u00b5m)]. Induced expression of Lamin A\/C \u00adcorrelated with areas of high wall stress from AAAs but was not significantly induced in the IMV from AAA patients compared to controls (n = 16). Stress-induced expression of Lamin A\/C was mimicked by exposing aortic smooth muscle cells to prolonged pulsatile stretch.<\/jats:p><jats:p>\n            \n              Conclusion:\n             Lamin A\/C protein is specifically increased in areas of high wall stress in AAA from patients, but is not increased on other vascular beds of aneurysm patients, suggesting that its elevation may be a compensatory response to the pathobiology leading to aneurysms.<\/jats:p>","DOI":"10.12945\/j.aorta.2015.14.069","type":"journal-article","created":{"date-parts":[[2016,4,30]],"date-time":"2016-04-30T20:42:14Z","timestamp":1462048934000},"page":"152-161","source":"Crossref","is-referenced-by-count":2,"title":["Increased Expression of Lamin A\/C Correlate with Regions of High Wall Stress in Abdominal Aortic Aneurysms"],"prefix":"10.1055","volume":"03","author":[{"given":"Amir","family":"Malkawi","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grisha","family":"Pirianov","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evelyn","family":"Torsney","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Chetter","sequence":"additional","affiliation":[{"name":"Centre for Cardiovascular & Metabolic Research, York Hull Medical School, Hull, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natzi","family":"Sakalihasan","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, University Hospital of Liege, Liege, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian M.","family":"Loftus","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian","family":"Nordon","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, University Hospital Southampton, Southampton, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Huggins","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicoletta","family":"Charolidi","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matt","family":"Thompson","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xie Yun","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Imperial College London, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gillian W.","family":"Cockerill","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Forensic Sciences, Anglia Ruskin University, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"194","published-online":{"date-parts":[[2018,9,24]]},"container-title":["AORTA"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.thieme-connect.de\/products\/ejournals\/pdf\/10.12945\/j.aorta.2015.14.069.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T20:52:05Z","timestamp":1557175925000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.thieme-connect.de\/DOI\/DOI?10.12945\/j.aorta.2015.14.069"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10]]},"references-count":0,"journal-issue":{"issue":"05","published-online":{"date-parts":[[2018,9,24]]},"published-print":{"date-parts":[[2015,10]]}},"URL":"https:\/\/doi.org\/10.12945\/j.aorta.2015.14.069","relation":{},"ISSN":["2325-4637"],"issn-type":[{"value":"2325-4637","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10]]}}}