{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T02:49:32Z","timestamp":1716605372294},"reference-count":42,"publisher":"Mark Allen Group","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Wound Care"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:sec><jats:title>Objective:<\/jats:title><jats:p>Wound healing can be delayed by the presence of colonising bacteria, and in polymicrobial wounds they may act synergistically to the further detriment of wound healing. In this pilot investigation, biopsy and swab samples were obtained as part of skin-graft operations performed on a chronic venous leg ulcer in order to study the spatial microbial diversity and to compare standard bacteriological and molecular biological techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method:<\/jats:title><jats:p>The wound was sampled before excision, and sampling was undertaken at multiple locations across the wound. Swab samples and biopsies were subjected to culture analysis and 16S rRNA polymerase chain reaction (PCR), and to denaturing gradient gel electrophoresis (DGGE).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Within the wound samples, DGGE identified the major wound microflora components and established the extent of local differences in bacterial diversity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p>This ongoing investigation has verified DGGE as a powerful tool for elucidating the clinical microbiology of a chronic disease state. It also suggests that skin graft operations are a novel way of obtaining multiple samples for in vivo bacteriology and for establishing the spatial distribution of bacteria in the complex micro-environment of chronic wounds.<\/jats:p><\/jats:sec><jats:sec><jats:title>Declaration of interest:<\/jats:title><jats:p>None.<\/jats:p><\/jats:sec>","DOI":"10.12968\/jowc.2007.16.4.27025","type":"journal-article","created":{"date-parts":[[2014,5,23]],"date-time":"2014-05-23T15:59:34Z","timestamp":1400860774000},"page":"171-175","source":"Crossref","is-referenced-by-count":24,"title":["Bacterial profiling using skin grafting, standard culture and molecular bacteriological methods"],"prefix":"10.12968","volume":"16","author":[{"given":"A.","family":"Andersen,","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Mycology and Parasitology, ABMP, Statens Serum Institut, Copenhagen, Denmark, Copenhagen Wound Healing Centre, Bispebjerg University Hospital, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.E.","family":"Hill","sequence":"additional","affiliation":[{"name":"Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Oral Surgery, Medicine and Pathology, Cardiff University, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Stephens","sequence":"additional","affiliation":[{"name":"Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Oral Surgery, Medicine and Pathology, Cardiff University, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.W.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Wound Biology Group, Cardiff Institute of Tissue Engineering and Repair, Oral Surgery, Medicine and Pathology, Cardiff University, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Jorgensen","sequence":"additional","affiliation":[{"name":"Copenhagen Wound Healing Centre, Bispebjerg University Hospital, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.A.","family":"Krogfelt","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Mycology and Parasitology, ABMP, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"5130","reference":[{"key":"p_1","volume-title":"Nutrition and the Prevention of Chronic Diseases","author":"World Health Organization","year":"2003"},{"key":"p_3","first-page":"5","volume":"187","year":"2004","journal-title":"Am J Surg"},{"key":"p_4","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1080\/00015555210317","volume":"210","author":"Agren M.S.","year":"2000","journal-title":"Acta Derm Venereol Suppl (Stockh)"},{"key":"p_6","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/S0094-1298(20)32468-8","volume":"25","author":"Cohen I.K","year":"1998","journal-title":"Clin Plast Surg"},{"key":"p_7","first-page":"44","volume":"49","author":"Bowler P.G","year":"2003","journal-title":"Ostomy Wound Manage"},{"key":"p_9","first-page":"8","volume":"49","author":"Bacterial","year":"2003","journal-title":"Ostomy Wound Manage"},{"key":"p_10","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2003.05232.x"},{"key":"p_12","doi-asserted-by":"publisher","DOI":"10.1046\/j.1524-475X.2002.t01-1-10602.x"},{"key":"p_13","doi-asserted-by":"publisher","DOI":"10.1016\/S1067-2516(97)80081-6"},{"key":"p_15","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh513"},{"key":"p_16","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3549-3557.2004"},{"key":"p_29","unstructured":"PCRfragments were separated using 9% (w\/v) polyacrylamide (acrylamide\/bisacrylamide 37.5:1; Bio-Rad) with 10% glycerol, containing a 25-60% linear gradient of denaturants (urea and formamide, Sigma) increasing in the direction of electrophoresis. 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