{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T22:50:41Z","timestamp":1772751041183,"version":"3.50.1"},"reference-count":45,"publisher":"Mark Allen Group","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Wound Care"],"published-print":{"date-parts":[[2018,12,2]]},"abstract":"<jats:sec><jats:title>Objective:<\/jats:title><jats:p> The amniotic membrane (AM) is a tissue with low immunogenity and high therapeutic potential due to its anti-inflammatory, anti-fibrotic and antimicrobial effects. This paper describes the use of cryopreserved amniotic membrane allografts to treat diabetic foot ulcers (DFUs) in patients with diabetes. <\/jats:p><\/jats:sec><jats:sec><jats:title>Method:<\/jats:title><jats:p> In this case series, AM was processed to obtain a final medicinal product: cryopreserved amniotic membrane. cryopreserved AM was applied every 7\u201310 days until total epithelialisation of the DFUs. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p> A total of 14 patients with DFUs (median size: 12.30cm, (range: 0.52\u201342.5cm<jats:sup>2<\/jats:sup>) were treated and followed up until complete closure (median time: 20 weeks, range: 7\u201356 weeks). Patients received 4\u201340 AM applications. All patients in this study achieved complete epithelialisation of the wound. No adverse events were observed. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p> AM is a feasible and safe treatment in complex DFUs. Furthermore, the treatment is successful in achieving epithelialisation of long-evolution, unhealed wounds resistant to conventional therapies. <\/jats:p><\/jats:sec>","DOI":"10.12968\/jowc.2018.27.12.806","type":"journal-article","created":{"date-parts":[[2018,12,17]],"date-time":"2018-12-17T17:45:05Z","timestamp":1545068705000},"page":"806-815","source":"Crossref","is-referenced-by-count":19,"title":["Cryopreserved amniotic membrane in the treatment of diabetic foot ulcers: a case series"],"prefix":"10.12968","volume":"27","author":[{"given":"M\u00f3nica Rodr\u00edguez","family":"Valiente","sequence":"first","affiliation":[{"name":"Cell Therapy Unit at Hospital Cl\u00ednico Universitario Virgen de la Arrixaca. Biomedical Research Institute of Murcia (Instituto Murciano de Investigaci\u00f3n Biosanitaria, IMIB-Arrixaca). El Palmar, Spain; and Department of General Surgery, Hospital Cl\u00ednico Universitario Virgen de la Arrixaca. Biomedical Research Institute of Murcia (Instituto Murciano de Investigaci\u00f3n Biosanitaria, IMIB-Arrixaca). El Palmar, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco J.","family":"Nicol\u00e1s","sequence":"additional","affiliation":[{"name":"Molecular Oncology and TGF\u00df, Research Unit at Hospital Cl\u00ednico Universitario Virgen de la Arrixaca. Biomedical Research Institute of Murcia (Instituto Murciano de Investigaci\u00f3n Biosanitaria, IMIB-Arrixaca)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana M.","family":"Garc\u00eda-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Cell Therapy Unit at Hospital Cl\u00ednico Universitario Virgen de la Arrixaca. Biomedical Research Institute of Murcia (Instituto Murciano de Investigaci\u00f3n Biosanitaria, IMIB-Arrixaca). 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