{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T08:00:03Z","timestamp":1781596803837,"version":"3.54.5"},"reference-count":19,"publisher":"Mark Allen Group","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Wound Care"],"published-print":{"date-parts":[[2018,2,2]]},"abstract":"<jats:sec><jats:title>Objective:<\/jats:title><jats:p>The Rigenera system is a new standardised micrograft preparation system. It works by means of automated mechanical disaggregation of small tissue samples, extracting only the smallest cells (&lt;50\u00b5m). The aim of this study was to retrospectively evaluate patients affected by chronic ulcers and who were treated with the micrograft preparation method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method:<\/jats:title><jats:p>Chronic ulcers have been included regardless of the cause. The specimen was collected with a 3mm diameter biopsy punch and immediately dissociated by means of the Rigenera System. The obtained suspension was placed on a scaffold of equine collagen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>We included 15 patients (four males, 11 females) with a mean age of 72.2\u00b18.41 (mean\u00b1standard deviation) years. In seven patients the ulcers were related to the complications of diabetes, post-traumatic in a further three diabetic patients, vasculitis in one patient, and four patients had venous leg ulcers (VLUs). The median main diameter was 5.0cm and the median estimated area was 43.96cm<jats:sup>2<\/jats:sup>. The ulcers were present from a mean of 4.50\u00b12.30 months before inclusion in this study. At the second week the wounds were reduced by 37.33%\u00b119.35%, at the week eight, nine patients (60.0%) were healed, and at week 16, 13 (86.7%) were healed. The quality of scars was good and did not deteriorate at the six month follow-up.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p>The simplicity of the approach, the minimal invasiveness of the specimen collection, and the good quality of scarring of healed wounds, confirmed in the follow-up, makes this micrograft preparation method a useful tool to use on large or complex wounds.<\/jats:p><\/jats:sec>","DOI":"10.12968\/jowc.2018.27.2.60","type":"journal-article","created":{"date-parts":[[2018,2,9]],"date-time":"2018-02-09T13:48:36Z","timestamp":1518184116000},"page":"60-65","source":"Crossref","is-referenced-by-count":19,"title":["Micrografting chronic lower extremity ulcers with mechanically disaggregated skin using a micrograft preparation system"],"prefix":"10.12968","volume":"27","author":[{"given":"Rosario","family":"Miranda","sequence":"first","affiliation":[{"name":"Medical Angiologist, Specialista Ambulatoriale Branca di Angiologia \u2013 Azienda Sanitaria Locale NA3-Sud Distretto di Nola (NA), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Farina","sequence":"additional","affiliation":[{"name":"Vascular Surgeon, Clinica Minerva \u2013 Santa Maria Capua Vetere (CE), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele A.","family":"Farina","sequence":"additional","affiliation":[{"name":"Vascular Surgeon, Clinica N. 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