{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T12:29:03Z","timestamp":1780489743923,"version":"3.54.1"},"reference-count":38,"publisher":"Mark Allen Group","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Wound Care"],"published-print":{"date-parts":[[2018,5,2]]},"abstract":"<jats:sec><jats:title>Objective:<\/jats:title><jats:p> To evaluate the clinical and microbiological effects of sequential wound debridement in a case series of neuroischaemic diabetic foot ulcers (DFUs) using an ultrasound-assisted wound debridement (UAW) device. <\/jats:p><\/jats:sec><jats:sec><jats:title>Method:<\/jats:title><jats:p> A prospective, single-centre study, involving a case series of 24 neuroischaemic DFUs, was conducted to evaluate sequential wound debridement with UAW during a six-week treatment period. Soft tissue punch biopsies were taken every second week of treatment, both before and after wound debridement sessions. Qualitative and quantitative microbiological analysis was performed and wounds were assessed at patient admission, and before and after each debridement procedure. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p> Wound tissue quality scores improved significantly from a mean score of 2.1\u00b11.3 points at patient inclusion, to 5.3\u00b11.7 points (p=0.001). Mean wound sizes were 4.45cm<jats:sup>2<\/jats:sup> (range: 2\u201312.25cm<jats:sup>2<\/jats:sup>) at week zero, and 2.75cm<jats:sup>2<\/jats:sup> (range: 1.67\u201310.70cm<jats:sup>2<\/jats:sup>) at week six (p=0.04). The mean number of bacterial species per culture determined at week zero and at week six was 2.53\u00b11.55 and 1.90\u00b11.16, respectively (p=0.023). Wound debridement resulted in significant decreases in bacterial counts (1.17, 1.31 and 0.77 log units in colony forming units (CFU) for week zero, three and six, respectively). The average bacterial load in tissue samples before and after wound debridement after the six-week treatment was Log 5.55\u00b10.91CFU\/g and Log 4.59\u00b10.89CFU\/g, respectively (p&lt;0.001). <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p> The study results showed a significant bacterial load reduction in DFU tissue samples as a result of UAW debridement, independent of bacterial species, some of which exhibited antibiotic-resistance. Significant bacterial load reduction was correlated with improved wound conditions and significant reductions of wound size. <\/jats:p><\/jats:sec>","DOI":"10.12968\/jowc.2018.27.5.278","type":"journal-article","created":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T17:48:28Z","timestamp":1525801708000},"page":"278-286","source":"Crossref","is-referenced-by-count":29,"title":["Ultrasound-assisted debridement of neuroischaemic diabetic foot ulcers, clinical and microbiological effects: a case series"],"prefix":"10.12968","volume":"27","author":[{"given":"Jos\u00e9 Luis","family":"L\u00e1zaro-Mart\u00ednez","sequence":"first","affiliation":[{"name":"Diabetic Foot Unit, Universidad Complutense de Madrid, Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdISSC), Madrid, Spain; Section of Microbiology, Department of Medicine, School of Medicine. 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