{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:57:03Z","timestamp":1790614623711,"version":"4.1.0"},"reference-count":24,"publisher":"Mark Allen Group","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Wound Care"],"published-print":{"date-parts":[[2021,4,2]]},"abstract":"<jats:sec><jats:title>Objective:<\/jats:title><jats:p>Fibroblasts have been shown to play an increasingly important role within diabetic wounds. While several in vitro models of diabetic wound fibroblasts have been reported, none replicate the natural progression of the disease over time, recapitulating the acquisition of the diseased phenotype. Therefore, this study aimed to establish an in vitro model of the diabetic wound fibroblast through sustained exposure of healthy dermal fibroblasts to hyperglycaemia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method:<\/jats:title><jats:p>Primary human fibroblasts were isolated from discarded healthy skin tissue and were either exposed to normoglycaemic (control 5.5mM glucose) media or hyperglycaemic (25mM glucose) media for four weeks. Quantitative polymerase chain reaction was performed to measure the expression of inflammatory cytokines and chemokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>In the hyperglycaemia model, stromal cell-derived factor (SDF)-1 expression remained consistently downregulated across all four weeks (p&lt;0.01), while monocyte chemoattractant protein (MCP)-1 (p&lt;0.001), interleukin (IL)-8 (p=0.847) and chemokine (C-X-C motif) ligand 1 (CXCL1) (p=0.872) were initially downregulated at one week followed by subsequent upregulation between 2\u20134 weeks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p>This hyperglycaemia model may serve as a useful tool to characterise pathological changes in the diabetic wound fibroblast and help identify candidate therapeutic targets, such as SDF-1, that may reverse the pathology.<\/jats:p><\/jats:sec>","DOI":"10.12968\/jowc.2021.30.4.300","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T17:27:00Z","timestamp":1618507620000},"page":"300-303","source":"Crossref","is-referenced-by-count":7,"title":["In vitro modelling of disease-induced changes in the diabetic wound fibroblast"],"prefix":"10.12968","volume":"30","author":[{"given":"Parviz","family":"Sorooshian","sequence":"first","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, Queen Victoria Hospital NHS Trust, East Grinstead, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony D","family":"Metcalfe","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering, University of Birmingham, Birmingham, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferdinand V","family":"Lali","sequence":"additional","affiliation":[{"name":"Northwick Park Institute for Medical Research, Northwick Park and St Mark's Hospital, Harrow, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5130","reference":[{"issue":"1","key":"B1","doi-asserted-by":"crossref","first-page":"S81","DOI":"10.2337\/dc14-S081","volume":"37","author":"American Diabetes Association","year":"2014","journal-title":"Diabetes Care"},{"issue":"7","key":"B2","doi-asserted-by":"crossref","first-page":"729","DOI":"10.3390\/cells8070729","volume":"8","author":"Ferroni L","year":"2019","journal-title":"Cells"},{"issue":"4","key":"B3","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1111\/wrr.12437","volume":"24","author":"Maione AG","year":"2016","journal-title":"Wound Repair Regen"},{"issue":"3","key":"B4","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1111\/j.1524-475X.2009.00488.x","volume":"17","author":"Harris KL","year":"2009","journal-title":"Wound Repair Regen"},{"issue":"1","key":"B5","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0923-1811(94)00355-I","volume":"9","author":"Yamada H","year":"1995","journal-title":"J Dermatol Sci"},{"issue":"5","key":"B6","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1089\/ten.tec.2014.0414","volume":"21","author":"Maione AG","year":"2015","journal-title":"Tissue Eng Part C Methods"},{"key":"B7","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.jconrel.2016.04.020","volume":"232","author":"Yeboah A","year":"2016","journal-title":"J Control Release"},{"issue":"5","key":"B8","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1111\/wrr.12334","volume":"23","author":"Olekson MA","year":"2015","journal-title":"Wound Repair Regen"},{"issue":"33","key":"B9","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1093\/eurheartj\/ehv254","volume":"36","author":"Chung ES","year":"2015","journal-title":"Eur Heart J"},{"issue":"3","key":"B10","first-page":"3715","volume":"17","author":"Wang R","year":"2018","journal-title":"Mol Med Rep"},{"issue":"5","key":"B11","doi-asserted-by":"crossref","first-page":"e97035","DOI":"10.1371\/journal.pone.0097035","volume":"9","author":"Jin Q","year":"2014","journal-title":"PLoS One"},{"issue":"2","key":"B12","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.jdermsci.2010.05.003","volume":"59","author":"Pukstad BS","year":"2010","journal-title":"J Dermatol Sci"},{"issue":"5","key":"B13","first-page":"1685","volume":"149","author":"Villarete LH","year":"1996","journal-title":"Am J Pathol"},{"issue":"1","key":"B14","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.jdiacomp.2015.10.002","volume":"30","author":"Singh K","year":"2016","journal-title":"J Diabetes Complications"},{"issue":"3","key":"B15","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1016\/j.jvs.2012.11.095","volume":"58","author":"Pradhan Nabzdyk L","year":"2013","journal-title":"J Vasc Surg"},{"issue":"2","key":"B16","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1007\/s11033-012-2223-6","volume":"40","author":"Schwarz F","year":"2013","journal-title":"Mol Biol Rep"},{"key":"B17","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.actbio.2015.12.023","volume":"32","author":"Kasiewicz LN","year":"2016","journal-title":"Acta Biomater"},{"issue":"6","key":"B18","doi-asserted-by":"crossref","first-page":"8193","DOI":"10.3892\/mmr.2015.4432","volume":"12","author":"Lin X","year":"2015","journal-title":"Mol Med Rep"},{"issue":"3","key":"B19","doi-asserted-by":"crossref","first-page":"159","DOI":"10.59566\/IJBS.2007.3159","volume":"3","author":"Morimoto H","year":"2007","journal-title":"Int J Biomed Sci"},{"issue":"5","key":"B20","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1159\/000363657","volume":"93","author":"Al-Amran FG","year":"2014","journal-title":"Pharmacology"},{"issue":"32","key":"B21","doi-asserted-by":"crossref","first-page":"13505","DOI":"10.1073\/pnas.0906670106","volume":"106","author":"Thangarajah H","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"issue":"5","key":"B22","first-page":"442","volume":"27","author":"Dhamodharan U","year":"2019","journal-title":"Circulatory levels of B-cell activating factor of the TNF family in patients with diabetic foot ulcer: association with disease progression"},{"issue":"1","key":"B23","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1002\/btm2.10123","volume":"4","author":"Kasiewicz LN","year":"2019","journal-title":"Bioeng Transl Med"},{"key":"B24","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.diabres.2019.02.024","volume":"150","author":"Wang T","year":"2019","journal-title":"Diabetes Res Clin Pract"}],"container-title":["Journal of Wound Care"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.magonlinelibrary.com\/doi\/pdf\/10.12968\/jowc.2021.30.4.300","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T12:37:33Z","timestamp":1724848653000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.magonlinelibrary.com\/doi\/10.12968\/jowc.2021.30.4.300"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,2]]},"references-count":24,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4,2]]}},"alternative-id":["10.12968\/jowc.2021.30.4.300"],"URL":"https:\/\/doi.org\/10.12968\/jowc.2021.30.4.300","relation":{},"ISSN":["0969-0700","2052-2916"],"issn-type":[{"value":"0969-0700","type":"print"},{"value":"2052-2916","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,2]]}}}