{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T11:57:58Z","timestamp":1791460678990,"version":"4.3.4"},"reference-count":42,"publisher":"Mark Allen Group","issue":"Sup10A","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Wound Care"],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction:<\/jats:title>\n                    <jats:p>Chronic wounds are difficult to manage because of their unpredictable outcome in terms of healing. Comparative analysis of tissue levels of matrix metalloproteinases MMP-2 and MMP-9 with blood levels of vascular endothelial growth factor (VEGF) in chronic wounds was carried out and followed up.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>A total of 52 patients were recruited to compare analysis of tissue levels of MMP-2 and MMP-9 with blood levels of VEGF in chronic wounds. Of these, 34 (65.4%) had diabetes. These patients had pressure ulcers grade 2\u20134 or wounds which had been present for &gt;6 weeks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Expression of VEGF was significantly increased in patients with chronic wounds (p&lt;0.001). The mean level of VEGF was 648.55\u00b1497.03pg\/ml at first visit and 965.69\u00b1461.51pg\/ml after 6 weeks, whereas the normal level is considered to be 31\u201386 pg\/ml. Levels of MMP-2 and MMP-9 were measured and assessed using immunohistochemistry at the time of first visit and after 6 weeks. Both were seen on keratinocytes, fibroblasts, inflammatory cells and endothelial cells at both time points. There was a significant increase in levels of both MMP-2 (p&lt;0.001) and MMP-9 (p&lt;0.001) in all of these cells after 6 weeks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Blood levels of VEGF and level of expression of MMP-2 and MMP-9 in tissues increased significantly with healing of chronic wounds.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.12968\/jowc.2022.0215","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T10:57:27Z","timestamp":1791457047000},"source":"Crossref","is-referenced-by-count":0,"title":["Comparative analysis of tissue levels of MMP-2 and MMP-9 with blood level of VEGF in chronic wounds"],"prefix":"10.12968","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7757-6302","authenticated-orcid":false,"given":"Satyendra K","family":"Tiwary","sequence":"first","affiliation":[{"name":"Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi","family":"Bilunia","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SP","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puneet","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajay K","family":"Khanna","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"5130","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32169"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.prs.0000225431.63010.1b"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-008-0078-y"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/1534734604273660"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63754-6"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199601)178:1<5::AID-PATH443>3.0.CO;2-W"},{"issue":"6","key":"e_1_3_2_7_2","first-page":"345","article-title":"Cytokine regulation of matrix metalloproteinase activity and its regulatory dysfunction in disease","volume":"376","author":"Ries C","year":"1995","unstructured":"Ries C, Petrides PE. 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