{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:46:55Z","timestamp":1791499615102,"version":"4.3.4"},"reference-count":47,"publisher":"Mark Allen Group","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Wound Care"],"published-print":{"date-parts":[[2022,7,2]]},"abstract":"<jats:sec><jats:title>Objective:<\/jats:title><jats:p> Wound dressings that inactivate or sequestrate microorganisms, such as those with a hydrophobic, bacteria-binding dialkylcarbamoyl chloride (DACC) surface, can reduce the risk of clinical infections. This \u2018passive\u2019 bioburden control, avoiding bacterial cell wall disruption with associated release of bacterial endotoxins aggravating inflammation, is advantageous in hard-to-heal wounds. Hence, the full scope of DACC dressings, including the potential impact of higher inoculum densities, increased protein load and different pH on antibacterial activity, needs to be evaluated. <\/jats:p><\/jats:sec><jats:sec><jats:title>Method:<\/jats:title><jats:p> The Japanese Industrial Standard (JIS) L 1902 challenge test was used to evaluate the antimicrobial activity of the DACC-coated dressing against several World Health Organization (WHO)-prioritised wound pathogens (e.g., meticillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus, microorganisms with extended-spectrum beta-lactamases and Acinetobacter baumannii), the effect of repeated bacterial challenge in an adverse wound environment, and antimicrobial performance at wound-related pH. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p> High antibacterial activity of the DACC-coated dressing against the WHO-prioritised bacteria strains by its irreversible binding and inhibition of growth of bound bacteria was confirmed using JIS L 1902. At increased inoculation densities, compared to standard conditions, the DACC-coated dressing still achieved strong-to-significant antibacterial effects. Augmenting the media protein content also affected antibacterial performance; a 0.5\u20131 log reduction in antibacterial activity was observed upon addition of 10% fetal calf serum. The pH did not influence antibacterial performance. The DACC-coated dressing also sustained antibacterial activity over subsequent reinfection steps. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p> It can be assumed that the DACC-coated dressing exerts beneficial effects in controlling the wound bioburden, reducing the overall demand placed on antibiotics, without using antimicrobial substances. <\/jats:p><\/jats:sec>","DOI":"10.12968\/jowc.2022.31.7.560","type":"journal-article","created":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T17:17:36Z","timestamp":1657214256000},"page":"560-570","source":"Crossref","is-referenced-by-count":19,"title":["Antimicrobial effects of bacterial binding to a dialkylcarbamoyl chloride-coated wound dressing: an in vitro study"],"prefix":"10.12968","volume":"31","author":[{"given":"Johanna","family":"Husmark","sequence":"first","affiliation":[{"name":"ABIGO Medical AB, Ekonomiv\u00e4gen 5, SE-436 33, Askim, 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