{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:01:01Z","timestamp":1752105661956,"version":"3.41.2"},"reference-count":27,"publisher":"Mark Allen Group","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Wound Care"],"published-print":{"date-parts":[[2025,7,2]]},"abstract":"<jats:sec><jats:title>Objective:<\/jats:title><jats:p> To report the effectiveness of allogeneic manual platelet separation and cryoprecipitate gel on hard-to-heal (chronic) wound healing in patients of older age. <\/jats:p><\/jats:sec><jats:sec><jats:title>Method:<\/jats:title><jats:p> Three patients aged 70\u201372 years were selected for this study. Among them, one had hard-to-heal diabetic foot ulcers (DFUs), one had developed large pressure injuries on the gluteal area, and one suffered from a purulent ulcer of the lower extremity. After thorough debridement, one unit (U) of allogeneic artificial platelets and 1\u20131.5U of cryoprecipitate were used to make the gel preparation which was subsequently applied to the wound surface every two to four days to treat these hard-to-heal wounds. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p> The wounds in the three patients had healed after 35\u201386 days with the application of the allogeneic manual platelet separation and cryoprecipitate gel. The patient with the large gluteal ulcer and sinus formation experienced rapid healing after applying the gel preparation for one month, while the patient with a DFU avoided high re-amputation after the gel preparation treatment. The patient with secondary acute myeloid leukaemia from myelodysplastic syndrome had their purulent wound completely healed after thorough debridement with the gel preparation treatment. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p> Manual separation of platelets and cryoprecipitate can effectively treat hard-to-heal ulcers or refractory wounds in elderly patients of older age as well as promote the quick development of granulation tissue and the wound-healing process. <\/jats:p><\/jats:sec>","DOI":"10.12968\/jowc.2023.0203","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T14:25:22Z","timestamp":1752071122000},"page":"488-495","source":"Crossref","is-referenced-by-count":0,"title":["Treatment of hard-to-heal ulcerative wounds with manually separated allogeneic platelets mixed with cryoprecipitate: three case reports"],"prefix":"10.12968","volume":"34","author":[{"given":"Wendong","family":"Ju","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Hematology, Zhongshan Boai Hospital Affiliated to Southern Medical University, Zhongshan 528403, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaoming","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Hematology, Zhongshan Boai Hospital Affiliated to Southern Medical University, Zhongshan 528403, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Ge","sequence":"additional","affiliation":[{"name":"Zhongshan City Central Blood Station of Guangdong, Zhongshan 528403, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Hematology, Zhongshan Boai Hospital Affiliated to Southern Medical University, Zhongshan 528403, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Hematology, Zhongshan Boai Hospital Affiliated to Southern Medical University, Zhongshan 528403, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Ou","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Hematology, Zhongshan Boai Hospital Affiliated to Southern Medical University, Zhongshan 528403, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Hematology, Zhongshan Boai Hospital Affiliated to Southern Medical University, Zhongshan 528403, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minmin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Hematology, Zhongshan Boai Hospital Affiliated to Southern Medical University, Zhongshan 528403, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyou","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Hematology, Zhongshan Boai Hospital Affiliated to Southern Medical University, Zhongshan 528403, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"5130","reference":[{"issue":"6","key":"B1","doi-asserted-by":"crossref","first-page":"e3160","DOI":"10.1002\/dmrr.3160","volume":"35","author":"Fu XL","year":"2019","journal-title":"Diabetes Metab Res Rev"},{"issue":"9498","key":"B2","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1016\/S0140-6736(05)67700-8","volume":"366","author":"Falanga V","year":"2005","journal-title":"Lancet"},{"issue":"4","key":"B3","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3390\/ph13040060","volume":"13","author":"Spampinato SF","year":"2020","journal-title":"Pharmaceuticals"},{"issue":"5","key":"B4","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1080\/14712598.2021.1897100","volume":"21","author":"Yarahmadi A","year":"2021","journal-title":"Expert Opin Biol Ther"},{"issue":"7","key":"B5","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1089\/wound.2018.0842","volume":"8","author":"Hu Z","year":"2019","journal-title":"Adv Wound Care"},{"issue":"2","key":"B6","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s10555-017-9677-x","volume":"36","author":"Holinstat M","year":"2017","journal-title":"Cancer Metastasis Rev"},{"key":"B7","first-page":"CD006899","volume":"10","author":"Martinez-Zapata MJ","year":"2012","journal-title":"Cochrane Database Syst Rev"},{"key":"B8","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.jss.2019.09.019","volume":"246","author":"Liao X","year":"2020","journal-title":"J Surg Res"},{"key":"B9","first-page":"29","author":"He M","year":"2020","journal-title":"Cell Transplant"},{"issue":"2","key":"B10","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1093\/milmed\/159.2.105","volume":"159","author":"Steinbaum SS","year":"1994","journal-title":"Mil Med"},{"issue":"4","key":"B11","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1111\/j.1742-481X.2008.00520.x","volume":"5","author":"Scholz T","year":"2008","journal-title":"Int Wound J"},{"key":"B12","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.addr.2020.09.005","volume":"170","author":"Patten J","year":"2021","journal-title":"Adv Drug Deliv Rev"},{"issue":"1","key":"B13","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1021\/acs.biomac.8b01258","volume":"20","author":"Mezzenga R","year":"2019","journal-title":"Biomacromolecules"},{"issue":"10","key":"B14","doi-asserted-by":"crossref","first-page":"eabj8331","DOI":"10.1126\/sciadv.abj8331","volume":"8","author":"Lickert S","year":"2022","journal-title":"Sci Adv"},{"issue":"1","key":"B15","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1177\/1534734603002001007","volume":"2","author":"Chauhan VS","year":"2003","journal-title":"Int J Low Extrem Wounds"},{"issue":"4","key":"B16","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1111\/iwj.12475","volume":"12","author":"Jones NJ","year":"2015","journal-title":"Int Wound J"},{"issue":"3","key":"B17","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1177\/1941738110366385","volume":"2","author":"Cole BJ","year":"2010","journal-title":"Sports Health: A Multidisciplinary Approach"},{"issue":"2","key":"B18","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1111\/wrr.12690","volume":"27","author":"del Pino-Sede\u00f1o T","year":"2019","journal-title":"Wound Repair Regen"},{"key":"B19","doi-asserted-by":"crossref","first-page":"716184","DOI":"10.3389\/fbioe.2021.716184","volume":"9","author":"Locatelli L","year":"2021","journal-title":"Front Bioeng Biotechnol"},{"issue":"6","key":"B20","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1093\/bja\/aeu158","volume":"113","author":"Nascimento B","year":"2014","journal-title":"Br J Anaesth"},{"issue":"1","key":"B21","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/BF01706323","volume":"16","author":"Perttil\u00e4 J","year":"1990","journal-title":"Intensive Care Med"},{"issue":"2","key":"B22","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1002\/jcb.240260206","volume":"26","author":"Grinnell F","year":"1984","journal-title":"J Cell Biochem"},{"issue":"5","key":"B23","doi-asserted-by":"crossref","first-page":"2738","DOI":"10.4049\/jimmunol.165.5.2738","volume":"165","author":"Franitza S","year":"2000","journal-title":"J Immunol"},{"key":"B24","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.3389\/fmicb.2016.01504","volume":"7","author":"Hymes JP","year":"2016","journal-title":"Front Microbiol"},{"issue":"2","key":"B25","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1001\/archderm.1988.01670020007002","volume":"124","author":"Wysocki A","year":"1988","journal-title":"Arch Dermatol"},{"issue":"4","key":"B26","doi-asserted-by":"crossref","first-page":"189","DOI":"10.4103\/0974-2077.150734","volume":"7","author":"Dhurat R","year":"2014","journal-title":"J Cutan Aesthet Surg"},{"issue":"1","key":"B27","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/s13071-020-3984-8","volume":"13","author":"Chagas CR","year":"2020","journal-title":"Parasit Vectors"}],"container-title":["Journal of Wound Care"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.magonlinelibrary.com\/doi\/pdf\/10.12968\/jowc.2023.0203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T14:25:45Z","timestamp":1752071145000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.magonlinelibrary.com\/doi\/10.12968\/jowc.2023.0203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,2]]},"references-count":27,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7,2]]}},"alternative-id":["10.12968\/jowc.2023.0203"],"URL":"https:\/\/doi.org\/10.12968\/jowc.2023.0203","relation":{},"ISSN":["0969-0700","2052-2916"],"issn-type":[{"value":"0969-0700","type":"print"},{"value":"2052-2916","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,2]]}}}