{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:06:16Z","timestamp":1790024776857,"version":"4.0.1"},"reference-count":39,"publisher":"Mark Allen Group","issue":"Sup3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Wound Care"],"published-print":{"date-parts":[[2025,3,1]]},"abstract":"<jats:sec><jats:title>Objective:<\/jats:title><jats:p> The aim of this study was to evaluate the effect on wound healing of intralesional epidermal growth factor (iEGF) (Heberprot-p; Hasbiotech, Cuba) and autologous adipose-derived tissue stromal vascular fraction (AD-tSVF) applied in the closure of tissue defects. <\/jats:p><\/jats:sec><jats:sec><jats:title>Method:<\/jats:title><jats:p> The patients included in the study were separated into three approximately equal groups: Group 1 with iEGF+AD-tSVF applied; Group 2 with iEGF only applied; and Group 3 with conventional wound care products applied. Granulation tissue was taken from the wound bed before the application of iEGF and AD-tSVF and at intervals thereafter for flow cytometry analysis. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p> Group 1 included 11 patients; Group 2 included 10 patients; and Group 3 included 10 patients. The time to re-epithelialisation was determined as 187.60\u00b168.78 days in Group 3 patients compared with Group 1 (72.27\u00b110.33 days) and Group 2 (70.50\u00b118.02 days) (p&lt;0.001). Following the application of iEGF to the wound bed, an increase was observed in M2 macrophage (CD209+), and M1 macrophage (CD38+) levels. The (CD34+) stem cells obtained from the granulation tissue after the application of AD-tSVF were determined to still be statistically significantly increased in the wound bed on the 21st day. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion:<\/jats:title><jats:p> The results of this study demonstrated that the application of iEGF and iEGF+ AD-tSVF significantly shortened the wound healing period compared with conventional methods. AD-tSVF stands as an effective option, especially in the patient group with halted or delayed wound healing despite the application of iEGF. Moreover, the significant increase (p&lt;0.001) in the level of M2 macrophages (CD209+), M1 macrophages (CD38+) and stem cells (CD34+) provided by this treatment modality showed that it contributed to wound healing at the cellular level. <\/jats:p><\/jats:sec>","DOI":"10.12968\/jowc.2024.0107","type":"journal-article","created":{"date-parts":[[2025,3,8]],"date-time":"2025-03-08T16:31:36Z","timestamp":1741451496000},"page":"xxx-xxxviii","source":"Crossref","is-referenced-by-count":3,"title":["Autologous adipose-derived tissue stromal vascular fraction and intralesional epidermal growth factor combined application in patients with diabetic foot"],"prefix":"10.12968","volume":"34","author":[{"given":"Ayd\u0131n","family":"Mustafa","sequence":"first","affiliation":[{"name":"MD, Orthopaedic Surgeon, Department of Orthopedics and Traumatology, University of Health Sciences Gulhane Training and Research Hospital, Ankara, 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