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Our in vitro study was designed to determine the maximum dose to which osteoblasts could be safely exposed.<\/jats:p><jats:p>Our findings demonstrated that zoledronate did not impair the proliferation of human osteoblasts when used at concentrations below 1 \u03bcm. Murine cells can be exposed to concentrations as high as 10 \u03bcm.<\/jats:p><jats:p>A concentration of 0.01% of titanium particles did not impair the proliferation of either cell line. Zoledronate affected the alkaline phosphatase activity of murine osteoblasts through a chelation phenomenon. The presence of titanium particles strongly decreased the alkaline phosphatase activity of murine osteoblasts. 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