{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:31:45Z","timestamp":1778589105297,"version":"3.51.4"},"reference-count":44,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Children's Orthopaedics"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>Surgical interventions are routinely performed on children with osteogenesis imperfecta (OI) to stabilize long bones, often post fracture. We speculated that a combination of intramedullary reaming and intraosseous injection of recombinant bone morphogenetic protein-2 (BMP-2) could enhance periosteal ossification and ultimately cortical thickness and strength. This approach was conceptually tested in a preclinical model of genetic bone fragility.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Six experimental groups were tested including no treatment, intramedullary reaming, and reaming with 5 \u00b5g BMP-2 injection performed in the tibiae of both wild type (WT) and Col1a2\n                      <jats:sup>G610C\/+<\/jats:sup>\n                      (OI, Amish mutation) mice. Bone formation was examined at a two-week time point in ex vivo specimens by micro-computed tomography (microCT) analysis and histomorphometry with a dynamic bone label.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MicroCT data illustrated increases in tibial cortical thickness with intramedullary reaming alone (Saline) and reaming plus BMP-2 injection (BMP-2) compared to no intervention controls. In the OI mice, the periosteal bone increase was not statistically significant with Saline but there was an increase of +192% (p = 0.053) with BMP-2 injection. Dynamic histomorphometry on calcein label was used to quantify new woven bone formation; while BMP-2 induced greater bone formation than Saline, the anabolic response was blunted overall in the OI groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These data indicate that targeting the intramedullary compartment via reaming and intraosseous BMP-2 delivery can lead to gains in cortical bone parameters. It is suggested that the next step is to validate safety and functional improvements in a clinical OI setting.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1302\/1863-2548.13.190119","type":"journal-article","created":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T07:23:01Z","timestamp":1570605781000},"page":"543-550","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Induction of periosteal bone formation by intraosseous BMP-2 injection in a mouse model of osteogenesis imperfecta"],"prefix":"10.1177","volume":"13","author":[{"given":"T. L.","family":"Cheng","sequence":"first","affiliation":[{"name":"Orthopaedic Research and Biotechnology Unit, Children's Hospital at Westmead, Sydney, NSW, Australia"},{"name":"Discipline of Child and Adolescent Health, Sydney Medical School, University of Sydney, Sydney, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. C.","family":"Cantrill","sequence":"additional","affiliation":[{"name":"Discipline of Child and Adolescent Health, Sydney Medical School, University of Sydney, Sydney, NSW, Australia"},{"name":"Microscopy Services at Kids Research, The Children's Hospital at Westmead, Sydney, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Schindeler","sequence":"additional","affiliation":[{"name":"Orthopaedic Research and Biotechnology Unit, Children's Hospital at Westmead, Sydney, NSW, Australia"},{"name":"Discipline of Child and Adolescent Health, Sydney Medical School, University of Sydney, Sydney, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. G.","family":"Little","sequence":"additional","affiliation":[{"name":"Orthopaedic Research and Biotechnology Unit, Children's Hospital at Westmead, Sydney, NSW, Australia"},{"name":"Discipline of Child and Adolescent Health, Sydney Medical School, University of Sydney, Sydney, NSW, 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