{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:22:46Z","timestamp":1761675766434},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We analyzed the performance of p16INK4a immunocytochemistry on a series of 810 retrospectively collected atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL) cases with available biopsy follow-up data, including 94 cases of cervical intraepithelial neoplasia (CIN) 2 and 128 cases of CIN 3. Human papillomavirus (HPV) testing was performed from the same residual liquid-based cytologic specimen, and results for both tests were correlated with histologic follow-up data. Sensitivity values for high-grade CIN (HGCIN) confirmed on biopsy within 6 months were 92.6% (ASC-US) and 92.2% (LSIL) for cytotechnologists\u2019 reviews of p16 cytology and 90.1% (ASC-US) and 95.7% (LSIL) for HPV testing. Sensitivity rates of initial pathologists\u2019 reviews were slightly lower, 76.4% to 80.1%, with levels comparable to cytotechnologists\u2019 results after adjudication. The specificity of p16 cytology for HGCIN detection was significantly higher than for HPV testing for cytotechnologists and pathologists: 63.2% to 71.1% (p16 cytology) vs 37.8% for HPV in ASC-US (P &amp;lt; .001) and 37.3% to 53.3% (p16 cytology) vs 18.5% for HPV in LSIL (P &amp;lt; .001). This evaluation of the diagnostic performance of p16 cytology confirms the potential of this stain for the efficient triage of ASC-US and LSIL cytologic results.<\/jats:p>","DOI":"10.1309\/ajcp3cd9ykyfjdql","type":"journal-article","created":{"date-parts":[[2010,6,15]],"date-time":"2010-06-15T16:59:18Z","timestamp":1276621158000},"page":"12-21","source":"Crossref","is-referenced-by-count":98,"title":["The Sensitivity and Specificity of p16INK4a Cytology vs HPV Testing for Detecting High-Grade Cervical Disease in the Triage of ASC-US and LSIL Pap Cytology Results"],"prefix":"10.1093","volume":"134","author":[{"given":"Karin J.","family":"Denton","sequence":"first","affiliation":[{"name":"1North Bristol NHS Trust, Bristol, England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Bergeron","sequence":"additional","affiliation":[{"name":"2Laboratoire Cerba, Cergy-Pontoise, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Klement","sequence":"additional","affiliation":[{"name":"3mtm laboratories, Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcus J.","family":"Trunk","sequence":"additional","affiliation":[{"name":"3mtm laboratories, Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Keller","sequence":"additional","affiliation":[{"name":"4Acomed statistik, Leipzig, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruediger","family":"Ridder","sequence":"additional","affiliation":[{"name":"3mtm laboratories, Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,7,1]]},"reference":[{"key":"2023011908213939700_b1-1340012","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1016\/S0140-6736(07)61416-0","article-title":"Human papillomavirus and cervical cancer","volume":"370","author":"Schiffman","year":"2007","journal-title":"Lancet"},{"key":"2023011908213939700_b2-1340012","doi-asserted-by":"crossref","first-page":"2120","DOI":"10.1001\/jama.287.16.2120","article-title":"2001 consensus guidelines for the management of women with cervical cytological abnormalities","volume":"287","author":"Wright","year":"2002","journal-title":"JAMA"},{"key":"2023011908213939700_b3-1340012","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1016\/S0002-9378(03)00418-6","article-title":"Results of a randomized trial on the management of cytology interpretations of atypical squamous cells of undetermined significance","volume":"188","author":"ALTS Study Group","year":"2003","journal-title":"Am J Obstet Gynecol"},{"key":"2023011908213939700_b4-1340012","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1093\/jnci\/93.4.293","article-title":"Comparison of three management strategies for patients with atypical squamous cells of undetermined significance: baseline results from a randomized trial","volume":"93","author":"Solomon","year":"2001","journal-title":"J Natl Cancer Inst"},{"key":"2023011908213939700_b5-1340012","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1097\/01.AOG.0000207557.30226.25","article-title":"Human papillomavirus triage for young women with atypical squamous cells of undetermined significance","volume":"107","author":"Wright","year":"2006","journal-title":"Obstet Gynecol"},{"key":"2023011908213939700_b6-1340012","doi-asserted-by":"crossref","first-page":"2536","DOI":"10.1158\/1055-9965.EPI-08-0306","article-title":"Human papillomavirus mRNA and p16 detection as biomarkers for the improved diagnosis of cervical neoplasia","volume":"17","author":"Cuschieri","year":"2008","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"2023011908213939700_b7-1340012","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1155\/2007\/678793","article-title":"Biomarkers in cervical cancer screening","volume":"23","author":"Wentzensen","year":"2007","journal-title":"Dis Markers"},{"key":"2023011908213939700_b8-1340012","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.ctrv.2008.10.005","article-title":"p16INK4a immunostaining in cytological and histological specimens from the uterine cervix: a systematic review and meta-analysis","volume":"35","author":"Tsoumpou","year":"2009","journal-title":"Cancer Treatment Rev"},{"key":"2023011908213939700_b9-1340012","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1309\/0DGGQBDQAMJCJBXB","article-title":"The predictive value of p16INK4a and Hybrid Capture 2 human papillomavirus testing for high-grade cervical intraepithelial neoplasia","volume":"122","author":"Guo","year":"2004","journal-title":"Am J Clin Pathol"},{"key":"2023011908213939700_b10-1340012","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1002\/cncr.20046","article-title":"Usefulness of liquid-based cytology specimens for the immunocytochemical study of p16 expression and human papillomavirus testing: a comparative study using simultaneously sampled histology materials","volume":"102","author":"Yoshida","year":"2004","journal-title":"Cancer"},{"key":"2023011908213939700_b11-1340012","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/j.ygyno.2005.06.060","article-title":"Which test is a better strategy to determine the outcome of atypical glandular cell\u2013categorized Pap smears? immunocytochemical p16INK4a expression or human papillomavirus test: a retrospective cohort study","volume":"99","author":"Chen","year":"2005","journal-title":"Gynecol Oncol"},{"key":"2023011908213939700_b12-1340012","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1002\/cncr.22420","article-title":"Triage of women with ASCUS and LSIL cytology: use of qualitative assessment of p16INK4a positive cells to identify patients with high-grade cervical intraepithelial neoplasia","volume":"111","author":"Wentzensen","year":"2007","journal-title":"Cancer"},{"key":"2023011908213939700_b13-1340012","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/cncr.22580","article-title":"Evaluation of p16INK4a expression in ThinPrep cervical specimens with the CINtec p16INK4a assay: correlation with biopsy follow-up results","volume":"111","author":"Meyer","year":"2007","journal-title":"Cancer"},{"key":"2023011908213939700_b14-1340012","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1002\/dc.20822","article-title":"Are adjunctive markers useful in routine cervical cancer screening? application of p16INK4a and HPV-PCR on ThinPrep samples with histological follow-up","volume":"36","author":"Schlederman","year":"2008","journal-title":"Diagn Cytopathol"},{"key":"2023011908213939700_b15-1340012","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1001\/jama.287.16.2114","article-title":"The 2001 Bethesda System: terminology for reporting results of cervical cytology","volume":"287","author":"Solomon","year":"2002","journal-title":"JAMA"},{"key":"2023011908213939700_b16-1340012","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1136\/jcp.39.9.933","article-title":"Terminology in gynaecological cytopathology: report of the Working Party of the British Society for Clinical Cytology","volume":"39","author":"Evans","year":"1986","journal-title":"J Clin Pathol"},{"key":"2023011908213939700_b17-1340012","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1002\/cncr.21378","article-title":"Evaluation of a nuclear score for p16INK4a-stained cervical squamous cells in liquid-based cytology samples","volume":"105","author":"Wentzensen","year":"2005","journal-title":"Cancer"},{"key":"2023011908213939700_b18-1340012","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780198509844.001.0001","volume-title":"The Statistical Evaluation of Medical Tests for Classification and Prediction","author":"Pepe","year":"2003"},{"key":"2023011908213939700_b19-1340012","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1002\/cncr.22284","article-title":"A comparison of the clinical utility of p16INK4a immunolocalization with the presence of human papillomavirus by Hybrid Capture 2 for the detection of cervical dysplasia\/neoplasia","volume":"108","author":"Holladay","year":"2006","journal-title":"Cancer"},{"key":"2023011908213939700_b20-1340012","doi-asserted-by":"crossref","first-page":"3033","DOI":"10.1158\/1055-9965.EPI-08-0508","article-title":"Comparison of predictors for high-grade cervical intraepithelial neoplasia in women with abnormal smears","volume":"17","author":"Szarewski","year":"2008","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"2023011908213939700_b21-1340012","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1159\/000326445","article-title":"Morphologic characteristics of p16INK4a-positive cells in cervical cytology samples","volume":"48","author":"Trunk","year":"2004","journal-title":"Acta Cytol"}],"container-title":["American Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/134\/1\/12\/48764267\/ajcp_134_1_12.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajcp\/article-pdf\/134\/1\/12\/48764267\/ajcp_134_1_12.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,27]],"date-time":"2024-03-27T16:27:40Z","timestamp":1711556860000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajcp\/article\/134\/1\/12\/1765779"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,1]]},"references-count":21,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2010,7,1]]},"published-print":{"date-parts":[[2010,7,1]]}},"URL":"https:\/\/doi.org\/10.1309\/ajcp3cd9ykyfjdql","relation":{},"ISSN":["1943-7722","0002-9173"],"issn-type":[{"value":"1943-7722","type":"electronic"},{"value":"0002-9173","type":"print"}],"subject":[],"published-other":{"date-parts":[[2010,7,1]]},"published":{"date-parts":[[2010,7,1]]}}}